Functional inhibition of katanin affects synaptic plasticity
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Functional inhibition of katanin affects synaptic plasticity. / Lombino, Franco L; Schwarz, Jürgen R; Pechmann, Yvonne; Schweizer, Michaela; Jark, Rebecca; Stange, Oliver; Glatzel, Markus; Gee, Christine E; Hausrat, Torben J; Gromova, Kira V; Kneussel, Matthias.
in: J NEUROSCI, Jahrgang 44, Nr. 13, e0374232023, 27.03.2024.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Functional inhibition of katanin affects synaptic plasticity
AU - Lombino, Franco L
AU - Schwarz, Jürgen R
AU - Pechmann, Yvonne
AU - Schweizer, Michaela
AU - Jark, Rebecca
AU - Stange, Oliver
AU - Glatzel, Markus
AU - Gee, Christine E
AU - Hausrat, Torben J
AU - Gromova, Kira V
AU - Kneussel, Matthias
N1 - Copyright © 2023 Lombino et al.
PY - 2024/3/27
Y1 - 2024/3/27
N2 - Dynamic microtubules critically regulate synaptic functions, but the role of microtubule severing in these processes is barely understood. Katanin is a neuronally expressed microtubule-severing complex regulating microtubule number and length in cell division or neurogenesis; however, its potential role in synaptic functions has remained unknown. Studying mice from both sexes, we found that katanin is abundant in neuronal dendrites and can be detected at individual excitatory spine synapses. Overexpression of a dominant-negative ATPase-deficient katanin subunit to functionally inhibit severing alters the growth of microtubules in dendrites, specifically at premature but not mature neuronal stages without affecting spine density. Notably, interference with katanin function prevented structural spine remodeling following single synapse glutamate uncaging and significantly affected the potentiation of AMPA-receptor-mediated excitatory currents after chemical induction of long-term potentiation. Furthermore, katanin inhibition reduced the invasion of microtubules into fully developed spines. Our data demonstrate that katanin-mediated microtubule severing regulates structural and functional plasticity at synaptic sites.
AB - Dynamic microtubules critically regulate synaptic functions, but the role of microtubule severing in these processes is barely understood. Katanin is a neuronally expressed microtubule-severing complex regulating microtubule number and length in cell division or neurogenesis; however, its potential role in synaptic functions has remained unknown. Studying mice from both sexes, we found that katanin is abundant in neuronal dendrites and can be detected at individual excitatory spine synapses. Overexpression of a dominant-negative ATPase-deficient katanin subunit to functionally inhibit severing alters the growth of microtubules in dendrites, specifically at premature but not mature neuronal stages without affecting spine density. Notably, interference with katanin function prevented structural spine remodeling following single synapse glutamate uncaging and significantly affected the potentiation of AMPA-receptor-mediated excitatory currents after chemical induction of long-term potentiation. Furthermore, katanin inhibition reduced the invasion of microtubules into fully developed spines. Our data demonstrate that katanin-mediated microtubule severing regulates structural and functional plasticity at synaptic sites.
U2 - 10.1523/JNEUROSCI.0374-23.2023
DO - 10.1523/JNEUROSCI.0374-23.2023
M3 - SCORING: Journal article
C2 - 38050126
VL - 44
JO - J NEUROSCI
JF - J NEUROSCI
SN - 0270-6474
IS - 13
M1 - e0374232023
ER -
