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Functional activity of RLIM/Rnf12 is regulated by phosphorylation-dependent nucleocytoplasmic shuttling

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Functional activity of RLIM/Rnf12 is regulated by phosphorylation-dependent nucleocytoplasmic shuttling. / Jiao, Baowei; Taniguchi-Ishigaki, Naoko; Güngör, Cenap; Peters, Marvin A; Chen, Ya-Wen; Riethdorf, Sabine; Drung, Alexander; Ahronian, Leanne G; Shin, Jongdae; Pagnis, Rachna; Pantel, Klaus; Tachibana, Taro; Lewis, Brian C; Johnsen, Steven A; Bach, Ingolf.

in: MOL BIOL CELL, Jahrgang 24, Nr. 19, 01.10.2013, S. 3085-96.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Jiao, B, Taniguchi-Ishigaki, N, Güngör, C, Peters, MA, Chen, Y-W, Riethdorf, S, Drung, A, Ahronian, LG, Shin, J, Pagnis, R, Pantel, K, Tachibana, T, Lewis, BC, Johnsen, SA & Bach, I 2013, 'Functional activity of RLIM/Rnf12 is regulated by phosphorylation-dependent nucleocytoplasmic shuttling', MOL BIOL CELL, Jg. 24, Nr. 19, S. 3085-96. https://doi.org/10.1091/mbc.E13-05-0239

APA

Jiao, B., Taniguchi-Ishigaki, N., Güngör, C., Peters, M. A., Chen, Y-W., Riethdorf, S., Drung, A., Ahronian, L. G., Shin, J., Pagnis, R., Pantel, K., Tachibana, T., Lewis, B. C., Johnsen, S. A., & Bach, I. (2013). Functional activity of RLIM/Rnf12 is regulated by phosphorylation-dependent nucleocytoplasmic shuttling. MOL BIOL CELL, 24(19), 3085-96. https://doi.org/10.1091/mbc.E13-05-0239

Vancouver

Jiao B, Taniguchi-Ishigaki N, Güngör C, Peters MA, Chen Y-W, Riethdorf S et al. Functional activity of RLIM/Rnf12 is regulated by phosphorylation-dependent nucleocytoplasmic shuttling. MOL BIOL CELL. 2013 Okt 1;24(19):3085-96. https://doi.org/10.1091/mbc.E13-05-0239

Bibtex

@article{0263506c7a0b4244a90b20039054b141,
title = "Functional activity of RLIM/Rnf12 is regulated by phosphorylation-dependent nucleocytoplasmic shuttling",
abstract = "The X-linked gene Rnf12 encodes the ubiquitin ligase really interesting new gene (RING) finger LIM domain-interacting protein (RLIM)/RING finger protein 12 (Rnf12), which serves as a major sex-specific epigenetic regulator of female mouse nurturing tissues. Early during embryogenesis, RLIM/Rnf12 expressed from the maternal allele is crucial for the development of extraembryonic trophoblast cells. In contrast, in mammary glands of pregnant and lactating adult females RLIM/Rnf12 expressed from the paternal allele functions as a critical survival factor for milk-producing alveolar cells. Although RLIM/Rnf12 is detected mostly in the nucleus, little is known about how and in which cellular compartment(s) RLIM/Rnf12 mediates its biological functions. Here we demonstrate that RLIM/Rnf12 protein shuttles between nucleus and cytoplasm and this is regulated by phosphorylation of serine S214 located within its nuclear localization sequence. We show that shuttling is important for RLIM to exert its biological functions, as alveolar cell survival activity is inhibited in cells expressing shuttling-deficient nuclear or cytoplasmic RLIM/Rnf12. Thus regulated nucleocytoplasmic shuttling of RLIM/Rnf12 coordinates cellular compartments during mammary alveolar cell survival.",
keywords = "Active Transport, Cell Nucleus, Animals, Cell Nucleus, Cell Survival, Embryonic Development, Epigenesis, Genetic, Female, HeLa Cells, Humans, LIM Domain Proteins, Mammary Glands, Animal, Mice, Phosphorylation, Pregnancy, Sex Determination Processes, Ubiquitin-Protein Ligases",
author = "Baowei Jiao and Naoko Taniguchi-Ishigaki and Cenap G{\"u}ng{\"o}r and Peters, {Marvin A} and Ya-Wen Chen and Sabine Riethdorf and Alexander Drung and Ahronian, {Leanne G} and Jongdae Shin and Rachna Pagnis and Klaus Pantel and Taro Tachibana and Lewis, {Brian C} and Johnsen, {Steven A} and Ingolf Bach",
year = "2013",
month = oct,
day = "1",
doi = "10.1091/mbc.E13-05-0239",
language = "English",
volume = "24",
pages = "3085--96",
journal = "MOL BIOL CELL",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "19",

}

RIS

TY - JOUR

T1 - Functional activity of RLIM/Rnf12 is regulated by phosphorylation-dependent nucleocytoplasmic shuttling

AU - Jiao, Baowei

AU - Taniguchi-Ishigaki, Naoko

AU - Güngör, Cenap

AU - Peters, Marvin A

AU - Chen, Ya-Wen

AU - Riethdorf, Sabine

AU - Drung, Alexander

AU - Ahronian, Leanne G

AU - Shin, Jongdae

AU - Pagnis, Rachna

AU - Pantel, Klaus

AU - Tachibana, Taro

AU - Lewis, Brian C

AU - Johnsen, Steven A

AU - Bach, Ingolf

PY - 2013/10/1

Y1 - 2013/10/1

N2 - The X-linked gene Rnf12 encodes the ubiquitin ligase really interesting new gene (RING) finger LIM domain-interacting protein (RLIM)/RING finger protein 12 (Rnf12), which serves as a major sex-specific epigenetic regulator of female mouse nurturing tissues. Early during embryogenesis, RLIM/Rnf12 expressed from the maternal allele is crucial for the development of extraembryonic trophoblast cells. In contrast, in mammary glands of pregnant and lactating adult females RLIM/Rnf12 expressed from the paternal allele functions as a critical survival factor for milk-producing alveolar cells. Although RLIM/Rnf12 is detected mostly in the nucleus, little is known about how and in which cellular compartment(s) RLIM/Rnf12 mediates its biological functions. Here we demonstrate that RLIM/Rnf12 protein shuttles between nucleus and cytoplasm and this is regulated by phosphorylation of serine S214 located within its nuclear localization sequence. We show that shuttling is important for RLIM to exert its biological functions, as alveolar cell survival activity is inhibited in cells expressing shuttling-deficient nuclear or cytoplasmic RLIM/Rnf12. Thus regulated nucleocytoplasmic shuttling of RLIM/Rnf12 coordinates cellular compartments during mammary alveolar cell survival.

AB - The X-linked gene Rnf12 encodes the ubiquitin ligase really interesting new gene (RING) finger LIM domain-interacting protein (RLIM)/RING finger protein 12 (Rnf12), which serves as a major sex-specific epigenetic regulator of female mouse nurturing tissues. Early during embryogenesis, RLIM/Rnf12 expressed from the maternal allele is crucial for the development of extraembryonic trophoblast cells. In contrast, in mammary glands of pregnant and lactating adult females RLIM/Rnf12 expressed from the paternal allele functions as a critical survival factor for milk-producing alveolar cells. Although RLIM/Rnf12 is detected mostly in the nucleus, little is known about how and in which cellular compartment(s) RLIM/Rnf12 mediates its biological functions. Here we demonstrate that RLIM/Rnf12 protein shuttles between nucleus and cytoplasm and this is regulated by phosphorylation of serine S214 located within its nuclear localization sequence. We show that shuttling is important for RLIM to exert its biological functions, as alveolar cell survival activity is inhibited in cells expressing shuttling-deficient nuclear or cytoplasmic RLIM/Rnf12. Thus regulated nucleocytoplasmic shuttling of RLIM/Rnf12 coordinates cellular compartments during mammary alveolar cell survival.

KW - Active Transport, Cell Nucleus

KW - Animals

KW - Cell Nucleus

KW - Cell Survival

KW - Embryonic Development

KW - Epigenesis, Genetic

KW - Female

KW - HeLa Cells

KW - Humans

KW - LIM Domain Proteins

KW - Mammary Glands, Animal

KW - Mice

KW - Phosphorylation

KW - Pregnancy

KW - Sex Determination Processes

KW - Ubiquitin-Protein Ligases

U2 - 10.1091/mbc.E13-05-0239

DO - 10.1091/mbc.E13-05-0239

M3 - SCORING: Journal article

C2 - 23904271

VL - 24

SP - 3085

EP - 3096

JO - MOL BIOL CELL

JF - MOL BIOL CELL

SN - 1059-1524

IS - 19

ER -