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Four-and-a-half LIM domain protein 2 is a novel regulator of sphingosine 1-phosphate receptor 1 in CCL19-induced dendritic cell migration

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Four-and-a-half LIM domain protein 2 is a novel regulator of sphingosine 1-phosphate receptor 1 in CCL19-induced dendritic cell migration. / König, Katharina; Diehl, Linda; Rommerscheidt-Fuss, Ursula; Golletz, Carsten; Quast, Thomas; Kahl, Philip; Kolanus, Waldemar; Knolle, Percy; Buettner, Reinhard; Heukamp, Lukas C.

in: J IMMUNOL, Jahrgang 185, Nr. 3, 01.08.2010, S. 1466-75.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

König, K, Diehl, L, Rommerscheidt-Fuss, U, Golletz, C, Quast, T, Kahl, P, Kolanus, W, Knolle, P, Buettner, R & Heukamp, LC 2010, 'Four-and-a-half LIM domain protein 2 is a novel regulator of sphingosine 1-phosphate receptor 1 in CCL19-induced dendritic cell migration', J IMMUNOL, Jg. 185, Nr. 3, S. 1466-75. https://doi.org/10.4049/jimmunol.0903449

APA

König, K., Diehl, L., Rommerscheidt-Fuss, U., Golletz, C., Quast, T., Kahl, P., Kolanus, W., Knolle, P., Buettner, R., & Heukamp, L. C. (2010). Four-and-a-half LIM domain protein 2 is a novel regulator of sphingosine 1-phosphate receptor 1 in CCL19-induced dendritic cell migration. J IMMUNOL, 185(3), 1466-75. https://doi.org/10.4049/jimmunol.0903449

Vancouver

König K, Diehl L, Rommerscheidt-Fuss U, Golletz C, Quast T, Kahl P et al. Four-and-a-half LIM domain protein 2 is a novel regulator of sphingosine 1-phosphate receptor 1 in CCL19-induced dendritic cell migration. J IMMUNOL. 2010 Aug 1;185(3):1466-75. https://doi.org/10.4049/jimmunol.0903449

Bibtex

@article{20a965799eab41e0b40ddad297361f2b,
title = "Four-and-a-half LIM domain protein 2 is a novel regulator of sphingosine 1-phosphate receptor 1 in CCL19-induced dendritic cell migration",
abstract = "We identified the four-and-a-half LIM domain protein 2 (FHL2) as a novel regulator of CCL19-induced dendritic cell (DC) migration. Initiation of migration is a hallmark of DC function and plays a central role in the induction and regulation of immune responses. In vivo, DCs continuously acquire Ag in the periphery and migrate to draining lymph nodes, under the influence of local environmental chemotactic factors like CCL19/21 or sphingosine 1-phosphate (S1P). We investigated the role of S1P- and RhoA-regulated FHL2 in this process. We found reduced nuclear localization of FHL2 in mature bone marrow-derived DCs (BMDCs), compared with immature BMDCs, following stimulation with CCL19. Furthermore, in vitro-generated murine FHL2(-/-) BMDCs displayed a significantly increased migratory speed, directionality, and migratory persistence toward the chemokine CCL19 compared with wild-type BMDCs. Moreover, in vivo, FHL2(-/-) BMDCs showed increased migration toward lymphoid organs. FHL2(-/-) BMDCs increased the expression of S1PR1, which was associated with greater Rac activation. An S1PR1 antagonist and knock-down of S1PR1 abrogated the increased migratory speed of FHL2(-/-) BMDCs. Our results identify FHL2 as an important novel regulator of DC migration via regulation of their sensitivity toward environmental migratory cues like S1P and CCL19.",
keywords = "Animals, Bone Marrow Cells, Cell Differentiation, Cell Movement, Cell Nucleus, Cells, Cultured, Chemokine CCL19, Dendritic Cells, Homeodomain Proteins, Immunophenotyping, LIM-Homeodomain Proteins, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Muscle Proteins, Receptors, Lysosphingolipid, Signal Transduction, Transcription Factors, Up-Regulation, rac GTP-Binding Proteins",
author = "Katharina K{\"o}nig and Linda Diehl and Ursula Rommerscheidt-Fuss and Carsten Golletz and Thomas Quast and Philip Kahl and Waldemar Kolanus and Percy Knolle and Reinhard Buettner and Heukamp, {Lukas C}",
year = "2010",
month = aug,
day = "1",
doi = "10.4049/jimmunol.0903449",
language = "English",
volume = "185",
pages = "1466--75",
journal = "J IMMUNOL",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "3",

}

RIS

TY - JOUR

T1 - Four-and-a-half LIM domain protein 2 is a novel regulator of sphingosine 1-phosphate receptor 1 in CCL19-induced dendritic cell migration

AU - König, Katharina

AU - Diehl, Linda

AU - Rommerscheidt-Fuss, Ursula

AU - Golletz, Carsten

AU - Quast, Thomas

AU - Kahl, Philip

AU - Kolanus, Waldemar

AU - Knolle, Percy

AU - Buettner, Reinhard

AU - Heukamp, Lukas C

PY - 2010/8/1

Y1 - 2010/8/1

N2 - We identified the four-and-a-half LIM domain protein 2 (FHL2) as a novel regulator of CCL19-induced dendritic cell (DC) migration. Initiation of migration is a hallmark of DC function and plays a central role in the induction and regulation of immune responses. In vivo, DCs continuously acquire Ag in the periphery and migrate to draining lymph nodes, under the influence of local environmental chemotactic factors like CCL19/21 or sphingosine 1-phosphate (S1P). We investigated the role of S1P- and RhoA-regulated FHL2 in this process. We found reduced nuclear localization of FHL2 in mature bone marrow-derived DCs (BMDCs), compared with immature BMDCs, following stimulation with CCL19. Furthermore, in vitro-generated murine FHL2(-/-) BMDCs displayed a significantly increased migratory speed, directionality, and migratory persistence toward the chemokine CCL19 compared with wild-type BMDCs. Moreover, in vivo, FHL2(-/-) BMDCs showed increased migration toward lymphoid organs. FHL2(-/-) BMDCs increased the expression of S1PR1, which was associated with greater Rac activation. An S1PR1 antagonist and knock-down of S1PR1 abrogated the increased migratory speed of FHL2(-/-) BMDCs. Our results identify FHL2 as an important novel regulator of DC migration via regulation of their sensitivity toward environmental migratory cues like S1P and CCL19.

AB - We identified the four-and-a-half LIM domain protein 2 (FHL2) as a novel regulator of CCL19-induced dendritic cell (DC) migration. Initiation of migration is a hallmark of DC function and plays a central role in the induction and regulation of immune responses. In vivo, DCs continuously acquire Ag in the periphery and migrate to draining lymph nodes, under the influence of local environmental chemotactic factors like CCL19/21 or sphingosine 1-phosphate (S1P). We investigated the role of S1P- and RhoA-regulated FHL2 in this process. We found reduced nuclear localization of FHL2 in mature bone marrow-derived DCs (BMDCs), compared with immature BMDCs, following stimulation with CCL19. Furthermore, in vitro-generated murine FHL2(-/-) BMDCs displayed a significantly increased migratory speed, directionality, and migratory persistence toward the chemokine CCL19 compared with wild-type BMDCs. Moreover, in vivo, FHL2(-/-) BMDCs showed increased migration toward lymphoid organs. FHL2(-/-) BMDCs increased the expression of S1PR1, which was associated with greater Rac activation. An S1PR1 antagonist and knock-down of S1PR1 abrogated the increased migratory speed of FHL2(-/-) BMDCs. Our results identify FHL2 as an important novel regulator of DC migration via regulation of their sensitivity toward environmental migratory cues like S1P and CCL19.

KW - Animals

KW - Bone Marrow Cells

KW - Cell Differentiation

KW - Cell Movement

KW - Cell Nucleus

KW - Cells, Cultured

KW - Chemokine CCL19

KW - Dendritic Cells

KW - Homeodomain Proteins

KW - Immunophenotyping

KW - LIM-Homeodomain Proteins

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Mice, Transgenic

KW - Muscle Proteins

KW - Receptors, Lysosphingolipid

KW - Signal Transduction

KW - Transcription Factors

KW - Up-Regulation

KW - rac GTP-Binding Proteins

U2 - 10.4049/jimmunol.0903449

DO - 10.4049/jimmunol.0903449

M3 - SCORING: Journal article

C2 - 20592280

VL - 185

SP - 1466

EP - 1475

JO - J IMMUNOL

JF - J IMMUNOL

SN - 0022-1767

IS - 3

ER -