Fluorescence staining of laryngeal neoplasms after topical application of 5-aminolevulinic acid: Preliminary results
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Fluorescence staining of laryngeal neoplasms after topical application of 5-aminolevulinic acid: Preliminary results. / Mehlmann, N; Betz, CS; Stepp, H; Arbogast, S; Baumgartner, R; Grevers, G; Leunig, A.
in: LASER SURG MED, Jahrgang 25, Nr. 5, 1999, S. 414-420.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Fluorescence staining of laryngeal neoplasms after topical application of 5-aminolevulinic acid: Preliminary results
AU - Mehlmann, N
AU - Betz, CS
AU - Stepp, H
AU - Arbogast, S
AU - Baumgartner, R
AU - Grevers, G
AU - Leunig, A
PY - 1999
Y1 - 1999
N2 - Background and Objective: The prognosis of patients suffering from laryngeal carcinomas can be improved by early diagnosis. Exact demarcation of tumor margins could contribute to an optimum preservation of the larynx. Therefore, the aim of the present study was the evaluation of 5-aminolevulinic acid (5-ALA)-induced protoporphyrin IX (PPIX) fluorescence as a new diagnostic procedure for the detection of laryngeal cancer. Study Design/Materials and Methods: Sixteen patients with suspected malignancies of the larynx received 0.6 wt% 5-ALA-NaCl solution by means of a medical nebulizer. After a period of 1-2 hours, the patients underwent microlaryngoscopy under white light and fluorescence illumination (lambda(ex) = 375-440 nm). A quantitative analysis of the fluorescence contrast between neoplastic and surrounding tissue was performed using an optical multichannel analyzer. Results: Carcinoma, carcinoma in situ, and dysplasia showed red fluorescence that could be attributed to the 5-ALA-induced formation of PPIX. The surrounding normal tissue exhibited autofluorescence in the green spectral range, which was greatly reduced within the tumor. The results of macroscopic red fluorescence staining were correlated with the histologic diagnosis. Conclusion: According to these preliminary results, the presented method seems to be a promising adjunct diagnostic procedure for the early identification of malignant neoplasms ill the larynx. The aim of further investigations is the assessment of sensitivity and specificity and an evaluation of fluorescence-guided laser resections of laryngeal cancer. Lasers Med. Surg. 25:414-420, 1999. (C) 1999 Wiley-Liss, inc.
AB - Background and Objective: The prognosis of patients suffering from laryngeal carcinomas can be improved by early diagnosis. Exact demarcation of tumor margins could contribute to an optimum preservation of the larynx. Therefore, the aim of the present study was the evaluation of 5-aminolevulinic acid (5-ALA)-induced protoporphyrin IX (PPIX) fluorescence as a new diagnostic procedure for the detection of laryngeal cancer. Study Design/Materials and Methods: Sixteen patients with suspected malignancies of the larynx received 0.6 wt% 5-ALA-NaCl solution by means of a medical nebulizer. After a period of 1-2 hours, the patients underwent microlaryngoscopy under white light and fluorescence illumination (lambda(ex) = 375-440 nm). A quantitative analysis of the fluorescence contrast between neoplastic and surrounding tissue was performed using an optical multichannel analyzer. Results: Carcinoma, carcinoma in situ, and dysplasia showed red fluorescence that could be attributed to the 5-ALA-induced formation of PPIX. The surrounding normal tissue exhibited autofluorescence in the green spectral range, which was greatly reduced within the tumor. The results of macroscopic red fluorescence staining were correlated with the histologic diagnosis. Conclusion: According to these preliminary results, the presented method seems to be a promising adjunct diagnostic procedure for the early identification of malignant neoplasms ill the larynx. The aim of further investigations is the assessment of sensitivity and specificity and an evaluation of fluorescence-guided laser resections of laryngeal cancer. Lasers Med. Surg. 25:414-420, 1999. (C) 1999 Wiley-Liss, inc.
KW - 5-aminolevulinic acid
KW - inhalation
KW - laryngeal neoplasms
KW - protoporphyrin
KW - spectroscopy
M3 - SCORING: Journal article
VL - 25
SP - 414
EP - 420
JO - LASER SURG MED
JF - LASER SURG MED
SN - 0196-8092
IS - 5
ER -