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Flow Cytometry of Membrane Purinoreceptors

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Flow Cytometry of Membrane Purinoreceptors. / Schwarz, Nicole; Junge, Marten; Haag, Friedrich; Koch-Nolte, Friedrich.

Purinergic Signaling: Methods and Protocols. Hrsg. / Pablo Pelegrín. 1. Aufl. New York, NY : Humana Press, 2020. S. 117-136 (Methods in Molecular Biology; Band 2041).

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Harvard

Schwarz, N, Junge, M, Haag, F & Koch-Nolte, F 2020, Flow Cytometry of Membrane Purinoreceptors. in P Pelegrín (Hrsg.), Purinergic Signaling: Methods and Protocols. 1 Aufl., Methods in Molecular Biology, Bd. 2041, Humana Press, New York, NY, S. 117-136. https://doi.org/10.1007/978-1-4939-9717-6_8

APA

Schwarz, N., Junge, M., Haag, F., & Koch-Nolte, F. (2020). Flow Cytometry of Membrane Purinoreceptors. in P. Pelegrín (Hrsg.), Purinergic Signaling: Methods and Protocols (1 Aufl., S. 117-136). (Methods in Molecular Biology; Band 2041). Humana Press. https://doi.org/10.1007/978-1-4939-9717-6_8

Vancouver

Schwarz N, Junge M, Haag F, Koch-Nolte F. Flow Cytometry of Membrane Purinoreceptors. in Pelegrín P, Hrsg., Purinergic Signaling: Methods and Protocols. 1 Aufl. New York, NY: Humana Press. 2020. S. 117-136. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9717-6_8

Bibtex

@inbook{949ab5a186c246858689c2983b43846c,
title = "Flow Cytometry of Membrane Purinoreceptors",
abstract = "Mammalian purinoreceptors respond to extracellular nucleotides and their metabolites, for example, following the release of ATP or NAD+ from cells and their hydrolysis by ectonucleotidases. Membrane purinoreceptors are expressed as ionotropic ligand-gated ion channels designated P2X receptors, or as metabotropic G-protein coupled receptors designated P1 or P2Y receptors, on the cell surface of different cell types. In this chapter, we provide protocols to monitor the expression and activity of purinoreceptors on the cell membrane of living cells by flow cytometry.",
keywords = "Adenosine Triphosphate/metabolism, Animals, Cell Membrane/metabolism, Flow Cytometry/methods, Humans, Nucleotides/metabolism, Receptors, Purinergic/metabolism, Signal Transduction",
author = "Nicole Schwarz and Marten Junge and Friedrich Haag and Friedrich Koch-Nolte",
year = "2020",
doi = "10.1007/978-1-4939-9717-6_8",
language = "English",
isbn = "978-1-4939-9716-9",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "117--136",
editor = "Pablo Pelegr{\'i}n",
booktitle = "Purinergic Signaling",
address = "United States",
edition = "1",

}

RIS

TY - CHAP

T1 - Flow Cytometry of Membrane Purinoreceptors

AU - Schwarz, Nicole

AU - Junge, Marten

AU - Haag, Friedrich

AU - Koch-Nolte, Friedrich

PY - 2020

Y1 - 2020

N2 - Mammalian purinoreceptors respond to extracellular nucleotides and their metabolites, for example, following the release of ATP or NAD+ from cells and their hydrolysis by ectonucleotidases. Membrane purinoreceptors are expressed as ionotropic ligand-gated ion channels designated P2X receptors, or as metabotropic G-protein coupled receptors designated P1 or P2Y receptors, on the cell surface of different cell types. In this chapter, we provide protocols to monitor the expression and activity of purinoreceptors on the cell membrane of living cells by flow cytometry.

AB - Mammalian purinoreceptors respond to extracellular nucleotides and their metabolites, for example, following the release of ATP or NAD+ from cells and their hydrolysis by ectonucleotidases. Membrane purinoreceptors are expressed as ionotropic ligand-gated ion channels designated P2X receptors, or as metabotropic G-protein coupled receptors designated P1 or P2Y receptors, on the cell surface of different cell types. In this chapter, we provide protocols to monitor the expression and activity of purinoreceptors on the cell membrane of living cells by flow cytometry.

KW - Adenosine Triphosphate/metabolism

KW - Animals

KW - Cell Membrane/metabolism

KW - Flow Cytometry/methods

KW - Humans

KW - Nucleotides/metabolism

KW - Receptors, Purinergic/metabolism

KW - Signal Transduction

U2 - 10.1007/978-1-4939-9717-6_8

DO - 10.1007/978-1-4939-9717-6_8

M3 - SCORING: Contribution to collected editions/anthologies

C2 - 31646484

SN - 978-1-4939-9716-9

T3 - Methods in Molecular Biology

SP - 117

EP - 136

BT - Purinergic Signaling

A2 - Pelegrín, Pablo

PB - Humana Press

CY - New York, NY

ER -