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First report of an Acanthamoeba genotype T13 isolate as etiological agent of a keratitis in humans

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First report of an Acanthamoeba genotype T13 isolate as etiological agent of a keratitis in humans. / Grün, Anna-Lena; Stemplewitz, Birthe; Scheid, Patrick.

in: PARASITOL RES, Jahrgang 113, Nr. 6, 2014, S. 2395-2400.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Grün, A-L, Stemplewitz, B & Scheid, P 2014, 'First report of an Acanthamoeba genotype T13 isolate as etiological agent of a keratitis in humans', PARASITOL RES, Jg. 113, Nr. 6, S. 2395-2400. https://doi.org/10.1007/s00436-014-3918-5

APA

Grün, A-L., Stemplewitz, B., & Scheid, P. (2014). First report of an Acanthamoeba genotype T13 isolate as etiological agent of a keratitis in humans. PARASITOL RES, 113(6), 2395-2400. https://doi.org/10.1007/s00436-014-3918-5

Vancouver

Grün A-L, Stemplewitz B, Scheid P. First report of an Acanthamoeba genotype T13 isolate as etiological agent of a keratitis in humans. PARASITOL RES. 2014;113(6):2395-2400. https://doi.org/10.1007/s00436-014-3918-5

Bibtex

@article{ad4c33d9363d4bac89f70e037e0949e0,
title = "First report of an Acanthamoeba genotype T13 isolate as etiological agent of a keratitis in humans",
abstract = "Several strains of free-living amoebae (FLA) belonging to the genus Acanthamoeba are able to cause a painful sight-threatening disease of the cornea designated as Acanthamoeba keratitis (AK). In this case report, a 22-year-old woman, wearer of soft contact lenses, was treated after the initial examination, and follow-up laboratory results led to the diagnosis of Acanthamoeba keratitis. The patient recovered under the targeted therapy, demonstrating that the acanthamoebae were the etiological agents of the keratitis in this case. The acanthamoebae belonged morphologically to group II. Genotyping of the causative Acanthamoeba strain based on sequences of the PCR amplimer ASA.S1 amplified from 18S ribosomal DNA by using the genus-specific primers JDP1 and JDP2 followed. The phylogenetic comparison of ASA.S1 confirmed that the isolated Acanthamoeba strain is closely related to genotype T13 supported by pairwise sequence identities of 97.1-98.0 % and bootstrap support of 980 replicates with reference sequences of genotype T13. These results regarding the Acanthamoeba keratitis-causing isolate KaBo expands the number of known pathogenic genotypes to 12. To our knowledge, this is the first report of a T13 Acanthamoeba genotype being associated with keratitis in humans.",
author = "Anna-Lena Gr{\"u}n and Birthe Stemplewitz and Patrick Scheid",
year = "2014",
doi = "10.1007/s00436-014-3918-5",
language = "English",
volume = "113",
pages = "2395--2400",
journal = "PARASITOL RES",
issn = "0932-0113",
publisher = "Springer",
number = "6",

}

RIS

TY - JOUR

T1 - First report of an Acanthamoeba genotype T13 isolate as etiological agent of a keratitis in humans

AU - Grün, Anna-Lena

AU - Stemplewitz, Birthe

AU - Scheid, Patrick

PY - 2014

Y1 - 2014

N2 - Several strains of free-living amoebae (FLA) belonging to the genus Acanthamoeba are able to cause a painful sight-threatening disease of the cornea designated as Acanthamoeba keratitis (AK). In this case report, a 22-year-old woman, wearer of soft contact lenses, was treated after the initial examination, and follow-up laboratory results led to the diagnosis of Acanthamoeba keratitis. The patient recovered under the targeted therapy, demonstrating that the acanthamoebae were the etiological agents of the keratitis in this case. The acanthamoebae belonged morphologically to group II. Genotyping of the causative Acanthamoeba strain based on sequences of the PCR amplimer ASA.S1 amplified from 18S ribosomal DNA by using the genus-specific primers JDP1 and JDP2 followed. The phylogenetic comparison of ASA.S1 confirmed that the isolated Acanthamoeba strain is closely related to genotype T13 supported by pairwise sequence identities of 97.1-98.0 % and bootstrap support of 980 replicates with reference sequences of genotype T13. These results regarding the Acanthamoeba keratitis-causing isolate KaBo expands the number of known pathogenic genotypes to 12. To our knowledge, this is the first report of a T13 Acanthamoeba genotype being associated with keratitis in humans.

AB - Several strains of free-living amoebae (FLA) belonging to the genus Acanthamoeba are able to cause a painful sight-threatening disease of the cornea designated as Acanthamoeba keratitis (AK). In this case report, a 22-year-old woman, wearer of soft contact lenses, was treated after the initial examination, and follow-up laboratory results led to the diagnosis of Acanthamoeba keratitis. The patient recovered under the targeted therapy, demonstrating that the acanthamoebae were the etiological agents of the keratitis in this case. The acanthamoebae belonged morphologically to group II. Genotyping of the causative Acanthamoeba strain based on sequences of the PCR amplimer ASA.S1 amplified from 18S ribosomal DNA by using the genus-specific primers JDP1 and JDP2 followed. The phylogenetic comparison of ASA.S1 confirmed that the isolated Acanthamoeba strain is closely related to genotype T13 supported by pairwise sequence identities of 97.1-98.0 % and bootstrap support of 980 replicates with reference sequences of genotype T13. These results regarding the Acanthamoeba keratitis-causing isolate KaBo expands the number of known pathogenic genotypes to 12. To our knowledge, this is the first report of a T13 Acanthamoeba genotype being associated with keratitis in humans.

U2 - 10.1007/s00436-014-3918-5

DO - 10.1007/s00436-014-3918-5

M3 - SCORING: Journal article

C2 - 24802868

VL - 113

SP - 2395

EP - 2400

JO - PARASITOL RES

JF - PARASITOL RES

SN - 0932-0113

IS - 6

ER -