Fibronectin coating of oxygenator membranes enhances endothelial cell attachment
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Fibronectin coating of oxygenator membranes enhances endothelial cell attachment. / Cornelissen, Christian G; Dietrich, Maren; Gromann, Kai; Frese, Julia; Krueger, Stefan; Sachweh, Jörg S; Jockenhoevel, Stefan.
in: BIOMED ENG ONLINE, Jahrgang 12, 28.01.2013, S. 7.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Fibronectin coating of oxygenator membranes enhances endothelial cell attachment
AU - Cornelissen, Christian G
AU - Dietrich, Maren
AU - Gromann, Kai
AU - Frese, Julia
AU - Krueger, Stefan
AU - Sachweh, Jörg S
AU - Jockenhoevel, Stefan
PY - 2013/1/28
Y1 - 2013/1/28
N2 - BACKGROUND: Extracorporeal membrane oxygenation (ECMO) can replace the lungs' gas exchange capacity in refractory lung failure. However, its limited hemocompatibility, the activation of the coagulation and complement system as well as plasma leakage and protein deposition hamper mid- to long-term use and have constrained the development of an implantable lung assist device. In a tissue engineering approach, lining the blood contact surfaces of the ECMO device with endothelial cells might overcome these limitations. As a first step towards this aim, we hypothesized that coating the oxygenator's gas exchange membrane with proteins might positively influence the attachment and proliferation of arterial endothelial cells.METHODS: Sheets of polypropylene (PP), polyoxymethylpentene (TPX) and polydimethylsiloxane (PDMS), typical material used for oxygenator gas exchange membranes, were coated with collagen, fibrinogen, gelatin or fibronectin. Tissue culture treated well plates served as controls. Endothelial cell attachment and proliferation were analyzed for a period of 4 days by microscopic examination and computer assisted cell counting.RESULTS: Endothelial cell seeding efficiency is within range of tissue culture treated controls for fibronectin treated surfaces only. Uncoated membranes as well as all other coatings lead to lower cell attachment. A confluent endothelial cell layer develops on fibronectin coated PDMS and the control surface only.CONCLUSIONS: Fibronectin increases endothelial cells' seeding efficiency on different oxygenator membrane material. PDMS coated with fibronectin shows sustained cell attachment for a period of four days in static culture conditions.
AB - BACKGROUND: Extracorporeal membrane oxygenation (ECMO) can replace the lungs' gas exchange capacity in refractory lung failure. However, its limited hemocompatibility, the activation of the coagulation and complement system as well as plasma leakage and protein deposition hamper mid- to long-term use and have constrained the development of an implantable lung assist device. In a tissue engineering approach, lining the blood contact surfaces of the ECMO device with endothelial cells might overcome these limitations. As a first step towards this aim, we hypothesized that coating the oxygenator's gas exchange membrane with proteins might positively influence the attachment and proliferation of arterial endothelial cells.METHODS: Sheets of polypropylene (PP), polyoxymethylpentene (TPX) and polydimethylsiloxane (PDMS), typical material used for oxygenator gas exchange membranes, were coated with collagen, fibrinogen, gelatin or fibronectin. Tissue culture treated well plates served as controls. Endothelial cell attachment and proliferation were analyzed for a period of 4 days by microscopic examination and computer assisted cell counting.RESULTS: Endothelial cell seeding efficiency is within range of tissue culture treated controls for fibronectin treated surfaces only. Uncoated membranes as well as all other coatings lead to lower cell attachment. A confluent endothelial cell layer develops on fibronectin coated PDMS and the control surface only.CONCLUSIONS: Fibronectin increases endothelial cells' seeding efficiency on different oxygenator membrane material. PDMS coated with fibronectin shows sustained cell attachment for a period of four days in static culture conditions.
KW - Animals
KW - Biocompatible Materials
KW - Cell Adhesion
KW - Collagen/chemistry
KW - Dimethylpolysiloxanes/chemistry
KW - Endothelial Cells/cytology
KW - Endothelium, Vascular/cytology
KW - Extracorporeal Membrane Oxygenation/methods
KW - Fibrinogen/chemistry
KW - Fibronectins/chemistry
KW - Gelatin/chemistry
KW - Oxygenators, Membrane
KW - Polypropylenes/chemistry
KW - Sheep
KW - Tissue Culture Techniques
KW - Tissue Engineering/methods
U2 - 10.1186/1475-925X-12-7
DO - 10.1186/1475-925X-12-7
M3 - SCORING: Journal article
C2 - 23356939
VL - 12
SP - 7
JO - BIOMED ENG ONLINE
JF - BIOMED ENG ONLINE
SN - 1475-925X
ER -
