F-actin patches associated with glutamatergic synapses control positioning of dendritic lysosomes
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F-actin patches associated with glutamatergic synapses control positioning of dendritic lysosomes. / van Bommel, Bas; Konietzny, Anja; Kobler, Oliver; Bär, Julia; Mikhaylova, Marina.
in: EMBO J, Jahrgang 38, Nr. 15, 01.08.2019, S. e101183.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - F-actin patches associated with glutamatergic synapses control positioning of dendritic lysosomes
AU - van Bommel, Bas
AU - Konietzny, Anja
AU - Kobler, Oliver
AU - Bär, Julia
AU - Mikhaylova, Marina
N1 - © 2019The Authors.
PY - 2019/8/1
Y1 - 2019/8/1
N2 - Organelle positioning within neurites is required for proper neuronal function. In dendrites, with their complex cytoskeletal organization, transport of organelles is guided by local specializations of the microtubule and actin cytoskeleton, and by coordinated activity of different motor proteins. Here, we focus on the actin cytoskeleton in the dendritic shaft and describe dense structures consisting of longitudinal and branched actin filaments. These actin patches are devoid of microtubules and are frequently located at the base of spines, or form an actin mesh around excitatory shaft synapses. Using lysosomes as an example, we demonstrate that the presence of actin patches has a strong impact on dendritic organelle transport, as lysosomes frequently stall at these locations. We provide mechanistic insights on this pausing behavior, demonstrating that actin patches form a physical barrier for kinesin-driven cargo. In addition, we identify myosin Va as an active tether which mediates long-term stalling. This correlation between the presence of actin meshes and halting of organelles could be a generalized principle by which synapses control organelle trafficking.
AB - Organelle positioning within neurites is required for proper neuronal function. In dendrites, with their complex cytoskeletal organization, transport of organelles is guided by local specializations of the microtubule and actin cytoskeleton, and by coordinated activity of different motor proteins. Here, we focus on the actin cytoskeleton in the dendritic shaft and describe dense structures consisting of longitudinal and branched actin filaments. These actin patches are devoid of microtubules and are frequently located at the base of spines, or form an actin mesh around excitatory shaft synapses. Using lysosomes as an example, we demonstrate that the presence of actin patches has a strong impact on dendritic organelle transport, as lysosomes frequently stall at these locations. We provide mechanistic insights on this pausing behavior, demonstrating that actin patches form a physical barrier for kinesin-driven cargo. In addition, we identify myosin Va as an active tether which mediates long-term stalling. This correlation between the presence of actin meshes and halting of organelles could be a generalized principle by which synapses control organelle trafficking.
U2 - 10.15252/embj.2018101183
DO - 10.15252/embj.2018101183
M3 - SCORING: Journal article
C2 - 31267565
VL - 38
SP - e101183
JO - EMBO J
JF - EMBO J
SN - 0261-4189
IS - 15
ER -