Extent of Inflammation and Foreign Body Reaction to Porous Polyethylene In Vitro and In Vivo
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Extent of Inflammation and Foreign Body Reaction to Porous Polyethylene In Vitro and In Vivo. / Vollkommer, Tobias; Henningsen, Anders; Friedrich, Reinhard E; Felthaus, Oliver Heinrich; Eder, Fabian; Morsczeck, Christian; Smeets, Ralf; Gehmert, Sebastian; Gosau, Martin.
in: IN VIVO, Jahrgang 33, Nr. 2, 26.02.2019, S. 337-347.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Extent of Inflammation and Foreign Body Reaction to Porous Polyethylene In Vitro and In Vivo
AU - Vollkommer, Tobias
AU - Henningsen, Anders
AU - Friedrich, Reinhard E
AU - Felthaus, Oliver Heinrich
AU - Eder, Fabian
AU - Morsczeck, Christian
AU - Smeets, Ralf
AU - Gehmert, Sebastian
AU - Gosau, Martin
N1 - Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
PY - 2019/2/26
Y1 - 2019/2/26
N2 - BACKGROUND/AIM: High-density porous polyethylene (PP) offers possibilities for reconstruction in craniofacial surgery. The purpose of this study was to evaluate the extent of inflammation and foreign body reactions to PP in vitro and in vivo.MATERIALS AND METHODS: Cell attachment, proliferation and expression of inflammatory cytokines were assessed using murine macrophages (RAW 264.7) on two different PP materials in vitro. In vivo, Balb/c mice received PP implants at their dorsum. After sacrifice, samples were analyzed histologically and real-time PCR was used to assess expression of inflammatory cytokines.RESULTS: Cells showed a significantly decreased proliferation (p<0.001) after 48 h and a significantly increased expression of TNF-α (p<0.05) at 24, 48 and 72 h. All animals showed foreign body cell reactions and signs of chronic inflammation. Expression of all but one of the investigated cytokines dropped to non-significant levels after an initial increase.CONCLUSION: Application of porous polyethylene can cause local chronic inflammatory reactions. Although clinical application seems to be immunologically safe, indication and risks should be evaluated carefully when using PP implants.
AB - BACKGROUND/AIM: High-density porous polyethylene (PP) offers possibilities for reconstruction in craniofacial surgery. The purpose of this study was to evaluate the extent of inflammation and foreign body reactions to PP in vitro and in vivo.MATERIALS AND METHODS: Cell attachment, proliferation and expression of inflammatory cytokines were assessed using murine macrophages (RAW 264.7) on two different PP materials in vitro. In vivo, Balb/c mice received PP implants at their dorsum. After sacrifice, samples were analyzed histologically and real-time PCR was used to assess expression of inflammatory cytokines.RESULTS: Cells showed a significantly decreased proliferation (p<0.001) after 48 h and a significantly increased expression of TNF-α (p<0.05) at 24, 48 and 72 h. All animals showed foreign body cell reactions and signs of chronic inflammation. Expression of all but one of the investigated cytokines dropped to non-significant levels after an initial increase.CONCLUSION: Application of porous polyethylene can cause local chronic inflammatory reactions. Although clinical application seems to be immunologically safe, indication and risks should be evaluated carefully when using PP implants.
KW - Animals
KW - Biocompatible Materials/administration & dosage
KW - Cytokines/genetics
KW - Foreign-Body Reaction/chemically induced
KW - Gene Expression Regulation/drug effects
KW - Inflammation/chemically induced
KW - Mice
KW - Mice, Inbred BALB C
KW - Polyethylene/administration & dosage
KW - Porosity/drug effects
KW - Prostheses and Implants/adverse effects
KW - RAW 264.7 Cells
KW - Tumor Necrosis Factor-alpha/genetics
U2 - 10.21873/invivo.11479
DO - 10.21873/invivo.11479
M3 - SCORING: Journal article
C2 - 30804110
VL - 33
SP - 337
EP - 347
JO - IN VIVO
JF - IN VIVO
SN - 0258-851X
IS - 2
ER -
