Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness

Standard

Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness. / Antonini, Andrea; Sattin, Andrea; Moroni, Monica; Bovetti, Serena; Moretti, Claudio; Succol, Francesca; Forli, Angelo; Vecchia, Dania; Rajamanickam, Vijayakumar P; Bertoncini, Andrea; Panzeri, Stefano; Liberale, Carlo; Fellin, Tommaso.

in: ELIFE, Jahrgang 9, e58882, 13.10.2020.

Publikationen: SCORING: Beitrag in Fachzeitschrift/ZeitungSCORING: ZeitschriftenaufsatzForschungBegutachtung

Harvard

Antonini, A, Sattin, A, Moroni, M, Bovetti, S, Moretti, C, Succol, F, Forli, A, Vecchia, D, Rajamanickam, VP, Bertoncini, A, Panzeri, S, Liberale, C & Fellin, T 2020, 'Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness', ELIFE, Jg. 9, e58882. https://doi.org/10.7554/eLife.58882

APA

Antonini, A., Sattin, A., Moroni, M., Bovetti, S., Moretti, C., Succol, F., Forli, A., Vecchia, D., Rajamanickam, V. P., Bertoncini, A., Panzeri, S., Liberale, C., & Fellin, T. (2020). Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness. ELIFE, 9, [e58882]. https://doi.org/10.7554/eLife.58882

Vancouver

Bibtex

@article{757c20eaf830455a9e7943e2f0de402e,
title = "Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness",
abstract = "Imaging neuronal activity with high and homogeneous spatial resolution across the field-of-view (FOV) and limited invasiveness in deep brain regions is fundamental for the progress of neuroscience, yet is a major technical challenge. We achieved this goal by correcting optical aberrations in gradient index lens-based ultrathin (≤500 µm) microendoscopes using aspheric microlenses generated through 3D-microprinting. Corrected microendoscopes had extended FOV (eFOV) with homogeneous spatial resolution for two-photon fluorescence imaging and required no modification of the optical set-up. Synthetic calcium imaging data showed that, compared to uncorrected endoscopes, eFOV-microendoscopes led to improved signal-to-noise ratio and more precise evaluation of correlated neuronal activity. We experimentally validated these predictions in awake head-fixed mice. Moreover, using eFOV-microendoscopes we demonstrated cell-specific encoding of behavioral state-dependent information in distributed functional subnetworks in a primary somatosensory thalamic nucleus. eFOV-microendoscopes are, therefore, small-cross-section ready-to-use tools for deep two-photon functional imaging with unprecedentedly high and homogeneous spatial resolution.",
keywords = "Animals, Behavior, Animal, Endoscopes, Female, Male, Mice, Mice, Inbred C57BL, Microscopy, Fluorescence, Multiphoton/instrumentation, Neurons/physiology, Thalamus/diagnostic imaging",
author = "Andrea Antonini and Andrea Sattin and Monica Moroni and Serena Bovetti and Claudio Moretti and Francesca Succol and Angelo Forli and Dania Vecchia and Rajamanickam, {Vijayakumar P} and Andrea Bertoncini and Stefano Panzeri and Carlo Liberale and Tommaso Fellin",
note = "{\textcopyright} 2020, Antonini et al.",
year = "2020",
month = oct,
day = "13",
doi = "10.7554/eLife.58882",
language = "English",
volume = "9",
journal = "ELIFE",
issn = "2050-084X",
publisher = "eLife Sciences Publications",

}

RIS

TY - JOUR

T1 - Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness

AU - Antonini, Andrea

AU - Sattin, Andrea

AU - Moroni, Monica

AU - Bovetti, Serena

AU - Moretti, Claudio

AU - Succol, Francesca

AU - Forli, Angelo

AU - Vecchia, Dania

AU - Rajamanickam, Vijayakumar P

AU - Bertoncini, Andrea

AU - Panzeri, Stefano

AU - Liberale, Carlo

AU - Fellin, Tommaso

N1 - © 2020, Antonini et al.

PY - 2020/10/13

Y1 - 2020/10/13

N2 - Imaging neuronal activity with high and homogeneous spatial resolution across the field-of-view (FOV) and limited invasiveness in deep brain regions is fundamental for the progress of neuroscience, yet is a major technical challenge. We achieved this goal by correcting optical aberrations in gradient index lens-based ultrathin (≤500 µm) microendoscopes using aspheric microlenses generated through 3D-microprinting. Corrected microendoscopes had extended FOV (eFOV) with homogeneous spatial resolution for two-photon fluorescence imaging and required no modification of the optical set-up. Synthetic calcium imaging data showed that, compared to uncorrected endoscopes, eFOV-microendoscopes led to improved signal-to-noise ratio and more precise evaluation of correlated neuronal activity. We experimentally validated these predictions in awake head-fixed mice. Moreover, using eFOV-microendoscopes we demonstrated cell-specific encoding of behavioral state-dependent information in distributed functional subnetworks in a primary somatosensory thalamic nucleus. eFOV-microendoscopes are, therefore, small-cross-section ready-to-use tools for deep two-photon functional imaging with unprecedentedly high and homogeneous spatial resolution.

AB - Imaging neuronal activity with high and homogeneous spatial resolution across the field-of-view (FOV) and limited invasiveness in deep brain regions is fundamental for the progress of neuroscience, yet is a major technical challenge. We achieved this goal by correcting optical aberrations in gradient index lens-based ultrathin (≤500 µm) microendoscopes using aspheric microlenses generated through 3D-microprinting. Corrected microendoscopes had extended FOV (eFOV) with homogeneous spatial resolution for two-photon fluorescence imaging and required no modification of the optical set-up. Synthetic calcium imaging data showed that, compared to uncorrected endoscopes, eFOV-microendoscopes led to improved signal-to-noise ratio and more precise evaluation of correlated neuronal activity. We experimentally validated these predictions in awake head-fixed mice. Moreover, using eFOV-microendoscopes we demonstrated cell-specific encoding of behavioral state-dependent information in distributed functional subnetworks in a primary somatosensory thalamic nucleus. eFOV-microendoscopes are, therefore, small-cross-section ready-to-use tools for deep two-photon functional imaging with unprecedentedly high and homogeneous spatial resolution.

KW - Animals

KW - Behavior, Animal

KW - Endoscopes

KW - Female

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Microscopy, Fluorescence, Multiphoton/instrumentation

KW - Neurons/physiology

KW - Thalamus/diagnostic imaging

U2 - 10.7554/eLife.58882

DO - 10.7554/eLife.58882

M3 - SCORING: Journal article

C2 - 33048047

VL - 9

JO - ELIFE

JF - ELIFE

SN - 2050-084X

M1 - e58882

ER -