Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness
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Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness. / Antonini, Andrea; Sattin, Andrea; Moroni, Monica; Bovetti, Serena; Moretti, Claudio; Succol, Francesca; Forli, Angelo; Vecchia, Dania; Rajamanickam, Vijayakumar P; Bertoncini, Andrea; Panzeri, Stefano; Liberale, Carlo; Fellin, Tommaso.
in: ELIFE, Jahrgang 9, e58882, 13.10.2020.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness
AU - Antonini, Andrea
AU - Sattin, Andrea
AU - Moroni, Monica
AU - Bovetti, Serena
AU - Moretti, Claudio
AU - Succol, Francesca
AU - Forli, Angelo
AU - Vecchia, Dania
AU - Rajamanickam, Vijayakumar P
AU - Bertoncini, Andrea
AU - Panzeri, Stefano
AU - Liberale, Carlo
AU - Fellin, Tommaso
N1 - © 2020, Antonini et al.
PY - 2020/10/13
Y1 - 2020/10/13
N2 - Imaging neuronal activity with high and homogeneous spatial resolution across the field-of-view (FOV) and limited invasiveness in deep brain regions is fundamental for the progress of neuroscience, yet is a major technical challenge. We achieved this goal by correcting optical aberrations in gradient index lens-based ultrathin (≤500 µm) microendoscopes using aspheric microlenses generated through 3D-microprinting. Corrected microendoscopes had extended FOV (eFOV) with homogeneous spatial resolution for two-photon fluorescence imaging and required no modification of the optical set-up. Synthetic calcium imaging data showed that, compared to uncorrected endoscopes, eFOV-microendoscopes led to improved signal-to-noise ratio and more precise evaluation of correlated neuronal activity. We experimentally validated these predictions in awake head-fixed mice. Moreover, using eFOV-microendoscopes we demonstrated cell-specific encoding of behavioral state-dependent information in distributed functional subnetworks in a primary somatosensory thalamic nucleus. eFOV-microendoscopes are, therefore, small-cross-section ready-to-use tools for deep two-photon functional imaging with unprecedentedly high and homogeneous spatial resolution.
AB - Imaging neuronal activity with high and homogeneous spatial resolution across the field-of-view (FOV) and limited invasiveness in deep brain regions is fundamental for the progress of neuroscience, yet is a major technical challenge. We achieved this goal by correcting optical aberrations in gradient index lens-based ultrathin (≤500 µm) microendoscopes using aspheric microlenses generated through 3D-microprinting. Corrected microendoscopes had extended FOV (eFOV) with homogeneous spatial resolution for two-photon fluorescence imaging and required no modification of the optical set-up. Synthetic calcium imaging data showed that, compared to uncorrected endoscopes, eFOV-microendoscopes led to improved signal-to-noise ratio and more precise evaluation of correlated neuronal activity. We experimentally validated these predictions in awake head-fixed mice. Moreover, using eFOV-microendoscopes we demonstrated cell-specific encoding of behavioral state-dependent information in distributed functional subnetworks in a primary somatosensory thalamic nucleus. eFOV-microendoscopes are, therefore, small-cross-section ready-to-use tools for deep two-photon functional imaging with unprecedentedly high and homogeneous spatial resolution.
KW - Animals
KW - Behavior, Animal
KW - Endoscopes
KW - Female
KW - Male
KW - Mice
KW - Mice, Inbred C57BL
KW - Microscopy, Fluorescence, Multiphoton/instrumentation
KW - Neurons/physiology
KW - Thalamus/diagnostic imaging
U2 - 10.7554/eLife.58882
DO - 10.7554/eLife.58882
M3 - SCORING: Journal article
C2 - 33048047
VL - 9
JO - ELIFE
JF - ELIFE
SN - 2050-084X
M1 - e58882
ER -
