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Expression of the human Cathepsin L inhibitor hurpin in mice: skin alterations and increased carcinogenesis.

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Expression of the human Cathepsin L inhibitor hurpin in mice: skin alterations and increased carcinogenesis. / Walz, Markus; Kellermann, Sabine; Bylaite, Matilda; Andrée, Birgit; Rüther, Ulrich; Paus, Ralf; Kloepper, Jennifer E; Reifenberger, Julia; Ruzicka, Thomas.

in: EXP DERMATOL, Jahrgang 16, Nr. 9, 9, 2007, S. 715-723.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Walz, M, Kellermann, S, Bylaite, M, Andrée, B, Rüther, U, Paus, R, Kloepper, JE, Reifenberger, J & Ruzicka, T 2007, 'Expression of the human Cathepsin L inhibitor hurpin in mice: skin alterations and increased carcinogenesis.', EXP DERMATOL, Jg. 16, Nr. 9, 9, S. 715-723. <http://www.ncbi.nlm.nih.gov/pubmed/17697143?dopt=Citation>

APA

Walz, M., Kellermann, S., Bylaite, M., Andrée, B., Rüther, U., Paus, R., Kloepper, J. E., Reifenberger, J., & Ruzicka, T. (2007). Expression of the human Cathepsin L inhibitor hurpin in mice: skin alterations and increased carcinogenesis. EXP DERMATOL, 16(9), 715-723. [9]. http://www.ncbi.nlm.nih.gov/pubmed/17697143?dopt=Citation

Vancouver

Walz M, Kellermann S, Bylaite M, Andrée B, Rüther U, Paus R et al. Expression of the human Cathepsin L inhibitor hurpin in mice: skin alterations and increased carcinogenesis. EXP DERMATOL. 2007;16(9):715-723. 9.

Bibtex

@article{ff9489eebbd347ae9d90a681d726b690,
title = "Expression of the human Cathepsin L inhibitor hurpin in mice: skin alterations and increased carcinogenesis.",
abstract = "The serine protease inhibitor (serpin) hurpin (serpin B13) is a cross class-specific inhibitor of the cysteine protease Cathepsin (Cat) L. Cat L is involved in lysosomal protein degradation, hair follicle morphogenesis, epidermal differentiation and epitope generation of antigens. Hurpin is a 44 kDa protein which is expressed predominantly in epidermal cells. In psoriatic skin samples, hurpin was strongly overexpressed when compared with normal skin. Keratinocytes overexpressing hurpin showed increased resistance towards UVB-induced apoptosis. To further analyse the functional importance of this inhibitor, we have generated transgenic mice with deregulated Cat L activity by expressing human hurpin in addition to the endogenous mouse inhibitor. The three independent transgenic lines generated were characterized by identical effects excluding insertional phenotypes. Macroscopically, mice expressing human hurpin are characterized by abnormal abdominal fur. The number of apoptotic cells and caspase-3 positive cells was reduced after UV-irradiation in transgenic animals compared with wild-type mice. Interestingly, after chemical carcinogenesis, transgenic mice showed an increased susceptibility to develop skin cancer. Array analysis of gene expression revealed distinct differences between wild-type and hurpin-transgenic mice. Among others, differentially expressed genes are related to antigen presentation and angiogenesis. These results suggest an important role of Cat L regulation by hurpin which might be of clinical relevance in human skin diseases.",
author = "Markus Walz and Sabine Kellermann and Matilda Bylaite and Birgit Andr{\'e}e and Ulrich R{\"u}ther and Ralf Paus and Kloepper, {Jennifer E} and Julia Reifenberger and Thomas Ruzicka",
year = "2007",
language = "Deutsch",
volume = "16",
pages = "715--723",
journal = "EXP DERMATOL",
issn = "0906-6705",
publisher = "Wiley-Blackwell",
number = "9",

}

RIS

TY - JOUR

T1 - Expression of the human Cathepsin L inhibitor hurpin in mice: skin alterations and increased carcinogenesis.

AU - Walz, Markus

AU - Kellermann, Sabine

AU - Bylaite, Matilda

AU - Andrée, Birgit

AU - Rüther, Ulrich

AU - Paus, Ralf

AU - Kloepper, Jennifer E

AU - Reifenberger, Julia

AU - Ruzicka, Thomas

PY - 2007

Y1 - 2007

N2 - The serine protease inhibitor (serpin) hurpin (serpin B13) is a cross class-specific inhibitor of the cysteine protease Cathepsin (Cat) L. Cat L is involved in lysosomal protein degradation, hair follicle morphogenesis, epidermal differentiation and epitope generation of antigens. Hurpin is a 44 kDa protein which is expressed predominantly in epidermal cells. In psoriatic skin samples, hurpin was strongly overexpressed when compared with normal skin. Keratinocytes overexpressing hurpin showed increased resistance towards UVB-induced apoptosis. To further analyse the functional importance of this inhibitor, we have generated transgenic mice with deregulated Cat L activity by expressing human hurpin in addition to the endogenous mouse inhibitor. The three independent transgenic lines generated were characterized by identical effects excluding insertional phenotypes. Macroscopically, mice expressing human hurpin are characterized by abnormal abdominal fur. The number of apoptotic cells and caspase-3 positive cells was reduced after UV-irradiation in transgenic animals compared with wild-type mice. Interestingly, after chemical carcinogenesis, transgenic mice showed an increased susceptibility to develop skin cancer. Array analysis of gene expression revealed distinct differences between wild-type and hurpin-transgenic mice. Among others, differentially expressed genes are related to antigen presentation and angiogenesis. These results suggest an important role of Cat L regulation by hurpin which might be of clinical relevance in human skin diseases.

AB - The serine protease inhibitor (serpin) hurpin (serpin B13) is a cross class-specific inhibitor of the cysteine protease Cathepsin (Cat) L. Cat L is involved in lysosomal protein degradation, hair follicle morphogenesis, epidermal differentiation and epitope generation of antigens. Hurpin is a 44 kDa protein which is expressed predominantly in epidermal cells. In psoriatic skin samples, hurpin was strongly overexpressed when compared with normal skin. Keratinocytes overexpressing hurpin showed increased resistance towards UVB-induced apoptosis. To further analyse the functional importance of this inhibitor, we have generated transgenic mice with deregulated Cat L activity by expressing human hurpin in addition to the endogenous mouse inhibitor. The three independent transgenic lines generated were characterized by identical effects excluding insertional phenotypes. Macroscopically, mice expressing human hurpin are characterized by abnormal abdominal fur. The number of apoptotic cells and caspase-3 positive cells was reduced after UV-irradiation in transgenic animals compared with wild-type mice. Interestingly, after chemical carcinogenesis, transgenic mice showed an increased susceptibility to develop skin cancer. Array analysis of gene expression revealed distinct differences between wild-type and hurpin-transgenic mice. Among others, differentially expressed genes are related to antigen presentation and angiogenesis. These results suggest an important role of Cat L regulation by hurpin which might be of clinical relevance in human skin diseases.

M3 - SCORING: Zeitschriftenaufsatz

VL - 16

SP - 715

EP - 723

JO - EXP DERMATOL

JF - EXP DERMATOL

SN - 0906-6705

IS - 9

M1 - 9

ER -