Expression of the B7-related molecule ICOSL by human glioma cells in vitro and in vivo
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Expression of the B7-related molecule ICOSL by human glioma cells in vitro and in vivo. / Schreiner, Bettina; Wischhusen, Jörg; Mitsdoerffer, Meike; Schneider, Dagmar; Bornemann, Antje; Melms, Arthur; Tolosa, Eva; Weller, Michael; Wiendl, Heinz.
in: GLIA, Jahrgang 44, Nr. 3, 01.12.2003, S. 296-301.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Expression of the B7-related molecule ICOSL by human glioma cells in vitro and in vivo
AU - Schreiner, Bettina
AU - Wischhusen, Jörg
AU - Mitsdoerffer, Meike
AU - Schneider, Dagmar
AU - Bornemann, Antje
AU - Melms, Arthur
AU - Tolosa, Eva
AU - Weller, Michael
AU - Wiendl, Heinz
N1 - Copyright 2003 Wiley-Liss, Inc.
PY - 2003/12/1
Y1 - 2003/12/1
N2 - Human glioblastoma is a highly lethal tumor known for its capability of interfering with effective antitumor immune responses. Costimulatory signals are of critical relevance in both the inductive and effector phases of immune responses. Inducible costimulator-ligand (ICOSL), a member of the B7 family of costimulatory molecules related to CD80/CD86, regulates CD4 as well as CD8 T-cell responses via interaction with its receptor, ICOS, on activated T cells. We report the expression of ICOSL by glioma cells in vitro and in vivo. In contrast to CD80 (B7.1) and CD86 (B7.2), ICOSL protein and mRNA was expressed in 7 of 12 glioma cell lines. ICOSL expression is upregulated by the inflammatory cytokine, tumor necrosis factor-alpha (TNF-alpha), whereas interferon-gamma (IFN-gamma) has no such effect. Further, immunohistochemical analysis of human brain tumors demonstrates the expression of ICOSL in three of four tissue samples. ICOSL expression is functional in that a neutralizing ICOSL antibody (HIL-131) reduces Th1 and Th2 cytokine levels in cocultures of peripheral blood lymphocytes or T-cell subsets (CD4 and CD8) with glioma cells. However, ICOSL gene transfer into glioma cells does not alter their immunogenicity under primary or secondary alloreactive coculture assays.
AB - Human glioblastoma is a highly lethal tumor known for its capability of interfering with effective antitumor immune responses. Costimulatory signals are of critical relevance in both the inductive and effector phases of immune responses. Inducible costimulator-ligand (ICOSL), a member of the B7 family of costimulatory molecules related to CD80/CD86, regulates CD4 as well as CD8 T-cell responses via interaction with its receptor, ICOS, on activated T cells. We report the expression of ICOSL by glioma cells in vitro and in vivo. In contrast to CD80 (B7.1) and CD86 (B7.2), ICOSL protein and mRNA was expressed in 7 of 12 glioma cell lines. ICOSL expression is upregulated by the inflammatory cytokine, tumor necrosis factor-alpha (TNF-alpha), whereas interferon-gamma (IFN-gamma) has no such effect. Further, immunohistochemical analysis of human brain tumors demonstrates the expression of ICOSL in three of four tissue samples. ICOSL expression is functional in that a neutralizing ICOSL antibody (HIL-131) reduces Th1 and Th2 cytokine levels in cocultures of peripheral blood lymphocytes or T-cell subsets (CD4 and CD8) with glioma cells. However, ICOSL gene transfer into glioma cells does not alter their immunogenicity under primary or secondary alloreactive coculture assays.
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Antigens, CD
KW - Antineoplastic Agents
KW - Brain Neoplasms
KW - CD8-Positive T-Lymphocytes
KW - Carrier Proteins
KW - Cell Line, Tumor
KW - Cells, Cultured
KW - Coculture Techniques
KW - Female
KW - Gene Expression Regulation, Neoplastic
KW - Glioblastoma
KW - Humans
KW - Inducible T-Cell Co-Stimulator Ligand
KW - Middle Aged
KW - Proteins
KW - Signal Transduction
KW - Tumor Necrosis Factor-alpha
U2 - 10.1002/glia.10291
DO - 10.1002/glia.10291
M3 - SCORING: Journal article
C2 - 14603470
VL - 44
SP - 296
EP - 301
JO - GLIA
JF - GLIA
SN - 0894-1491
IS - 3
ER -