Expression of selectin ligands on murine effector and IL-10-producing CD4+ T cells from non-infected and infected tissues
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Expression of selectin ligands on murine effector and IL-10-producing CD4+ T cells from non-infected and infected tissues. / Kretschmer, Ute; Bonhagen, Kerstin; Debes, Gudrun F; Mittrücker, Hans-Willi; Erb, Klaus J; Liesenfeld, Oliver; Zaiss, Dietmar; Kamradt, Thomas; Syrbe, Uta; Hamann, Alf.
in: EUR J IMMUNOL, Jahrgang 34, Nr. 11, 01.11.2004, S. 3070-81.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Expression of selectin ligands on murine effector and IL-10-producing CD4+ T cells from non-infected and infected tissues
AU - Kretschmer, Ute
AU - Bonhagen, Kerstin
AU - Debes, Gudrun F
AU - Mittrücker, Hans-Willi
AU - Erb, Klaus J
AU - Liesenfeld, Oliver
AU - Zaiss, Dietmar
AU - Kamradt, Thomas
AU - Syrbe, Uta
AU - Hamann, Alf
PY - 2004/11/1
Y1 - 2004/11/1
N2 - Endothelial selectins are crucial for the recruitment of leukocytes into sites of inflammation. On T cells, ligands for selectins become induced upon differentiation into the effector/memory stage. Initial in vitro studies suggested a correlation between the Th1 phenotype and ligand expression, but whether this also holds true in vivo remained uncertain. We here analyzed selectin ligands on CD4+ T cells producing IFN-gamma, IL-4 or IL-10, prototypic cytokines of the Th1, Th2 and Tr1 subset, respectively. We analyzed mice infected with influenza virus, the bacterium Listeria, and the parasites Toxoplasma (all Th1 models) or Nippostrongylus (Th2 model). A link between the Th1 phenotype and ligand expression was not found in vivo. Surprisingly, the potentially regulatory IL-10-producing T cells displayed the highest frequency of ligand-positive cells in general. Within the inflamed tissues, the frequencies of P-selectin-binding cells increased in the dominant subset, either Th1 or Th2. Up-regulation was also found for E-selectin ligands during influenza, but not Nippostrongylus infection. In conclusion, conditions driving T cell polarization into either Th1 or Th2 in vivo do not affect the expression of selectin ligands, but acquisition of P-selectin binding and hence migration into inflamed tissues is boosted by an inflammatory milieu.
AB - Endothelial selectins are crucial for the recruitment of leukocytes into sites of inflammation. On T cells, ligands for selectins become induced upon differentiation into the effector/memory stage. Initial in vitro studies suggested a correlation between the Th1 phenotype and ligand expression, but whether this also holds true in vivo remained uncertain. We here analyzed selectin ligands on CD4+ T cells producing IFN-gamma, IL-4 or IL-10, prototypic cytokines of the Th1, Th2 and Tr1 subset, respectively. We analyzed mice infected with influenza virus, the bacterium Listeria, and the parasites Toxoplasma (all Th1 models) or Nippostrongylus (Th2 model). A link between the Th1 phenotype and ligand expression was not found in vivo. Surprisingly, the potentially regulatory IL-10-producing T cells displayed the highest frequency of ligand-positive cells in general. Within the inflamed tissues, the frequencies of P-selectin-binding cells increased in the dominant subset, either Th1 or Th2. Up-regulation was also found for E-selectin ligands during influenza, but not Nippostrongylus infection. In conclusion, conditions driving T cell polarization into either Th1 or Th2 in vivo do not affect the expression of selectin ligands, but acquisition of P-selectin binding and hence migration into inflamed tissues is boosted by an inflammatory milieu.
KW - Animals
KW - CD4-Positive T-Lymphocytes
KW - Cytokines
KW - Female
KW - Gene Expression Regulation
KW - Immunologic Memory
KW - Influenza A virus
KW - Interleukin-10
KW - Ligands
KW - Listeriosis
KW - Lung Diseases
KW - Mice
KW - Mice, Inbred BALB C
KW - Mice, Inbred C57BL
KW - Nippostrongylus
KW - Orthomyxoviridae Infections
KW - Selectins
KW - Specific Pathogen-Free Organisms
KW - Strongylida Infections
KW - T-Lymphocytes, Regulatory
KW - Toxoplasmosis
U2 - 10.1002/eji.200424972
DO - 10.1002/eji.200424972
M3 - SCORING: Journal article
C2 - 15384048
VL - 34
SP - 3070
EP - 3081
JO - EUR J IMMUNOL
JF - EUR J IMMUNOL
SN - 0014-2980
IS - 11
ER -