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Expression of miRNAs miR-133b and miR-206 in the Il17a/f locus is co-regulated with IL-17 production in αβ and γδ T cells

Standard

Expression of miRNAs miR-133b and miR-206 in the Il17a/f locus is co-regulated with IL-17 production in αβ and γδ T cells. / Haas, Jan D; Nistala, Kiran; Petermann, Franziska; Saran, Namita; Chennupati, Vijaykumar; Schmitz, Susanne; Korn, Thomas; Wedderburn, Lucy R; Förster, Reinhold; Krueger, Andreas; Prinz, Immo.

in: PLOS ONE, Jahrgang 6, Nr. 5, 2011, S. e20171.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Haas, JD, Nistala, K, Petermann, F, Saran, N, Chennupati, V, Schmitz, S, Korn, T, Wedderburn, LR, Förster, R, Krueger, A & Prinz, I 2011, 'Expression of miRNAs miR-133b and miR-206 in the Il17a/f locus is co-regulated with IL-17 production in αβ and γδ T cells', PLOS ONE, Jg. 6, Nr. 5, S. e20171. https://doi.org/10.1371/journal.pone.0020171

APA

Haas, J. D., Nistala, K., Petermann, F., Saran, N., Chennupati, V., Schmitz, S., Korn, T., Wedderburn, L. R., Förster, R., Krueger, A., & Prinz, I. (2011). Expression of miRNAs miR-133b and miR-206 in the Il17a/f locus is co-regulated with IL-17 production in αβ and γδ T cells. PLOS ONE, 6(5), e20171. https://doi.org/10.1371/journal.pone.0020171

Vancouver

Haas JD, Nistala K, Petermann F, Saran N, Chennupati V, Schmitz S et al. Expression of miRNAs miR-133b and miR-206 in the Il17a/f locus is co-regulated with IL-17 production in αβ and γδ T cells. PLOS ONE. 2011;6(5):e20171. https://doi.org/10.1371/journal.pone.0020171

Bibtex

@article{83e9d4540a674034bccdee17749b8d0a,
title = "Expression of miRNAs miR-133b and miR-206 in the Il17a/f locus is co-regulated with IL-17 production in αβ and γδ T cells",
abstract = "Differentiation of T helper 17 cells (Th17) is a multistep process that involves the cytokines IL-6, TGF-β, and IL-23 as well as IL-1β, IL-21, and TNF-α. Thereby, robust induction of the capacity to produce IL-17 involves epigenetic modifications of the syntenic Il17a/f locus. Using inbred mouse strains, we identified co-regulation of gene transcription at the Il17a/f locus with the nearby microRNAs miR-133b and miR-206 that are clustered approximately 45 kb upstream of Il17a/f. Expression of these microRNAs was specific for Th17 as compared to other CD4(+) T cell subsets and this was equally valid for in vitro polarized and ex vivo derived cells. From all factors analyzed, IL-23 was the most important cytokine for the in vitro induction of miR-133b and miR-206 in naive CD4(+) T cells of wild type mice. However, analysis of IL-23R deficient mice revealed that IL-23R signaling was not essential for the induction of miR-133b and miR-206. Importantly, we found a similar co-regulation in CCR6(+) and other γδ T cell subsets that are predisposed to production of IL-17. Taken together, we discovered a novel feature of T cell differentiation towards an IL-17-producing phenotype that is shared between αβ and γδ T cells. Notably, the specific co-regulation of miR-133b and miR-206 with the Il17a/f locus also extended to human Th17 cells. This qualifies expression of miR-133b and miR-206 in T cells as novel biomarkers for Th17-type immune reactions.",
keywords = "Animals, Base Sequence, Cell Differentiation/genetics, Cell Polarity/genetics, Cells, Cultured, Gene Expression Regulation, Genetic Loci, Humans, Interleukin-17/biosynthesis, Interleukin-23/metabolism, Mice, MicroRNAs/genetics, Molecular Sequence Data, Receptors, Antigen, T-Cell, alpha-beta/metabolism, Receptors, Antigen, T-Cell, gamma-delta/metabolism, Synteny/genetics, Th17 Cells/cytology",
author = "Haas, {Jan D} and Kiran Nistala and Franziska Petermann and Namita Saran and Vijaykumar Chennupati and Susanne Schmitz and Thomas Korn and Wedderburn, {Lucy R} and Reinhold F{\"o}rster and Andreas Krueger and Immo Prinz",
year = "2011",
doi = "10.1371/journal.pone.0020171",
language = "English",
volume = "6",
pages = "e20171",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

RIS

TY - JOUR

T1 - Expression of miRNAs miR-133b and miR-206 in the Il17a/f locus is co-regulated with IL-17 production in αβ and γδ T cells

AU - Haas, Jan D

AU - Nistala, Kiran

AU - Petermann, Franziska

AU - Saran, Namita

AU - Chennupati, Vijaykumar

AU - Schmitz, Susanne

AU - Korn, Thomas

AU - Wedderburn, Lucy R

AU - Förster, Reinhold

AU - Krueger, Andreas

AU - Prinz, Immo

PY - 2011

Y1 - 2011

N2 - Differentiation of T helper 17 cells (Th17) is a multistep process that involves the cytokines IL-6, TGF-β, and IL-23 as well as IL-1β, IL-21, and TNF-α. Thereby, robust induction of the capacity to produce IL-17 involves epigenetic modifications of the syntenic Il17a/f locus. Using inbred mouse strains, we identified co-regulation of gene transcription at the Il17a/f locus with the nearby microRNAs miR-133b and miR-206 that are clustered approximately 45 kb upstream of Il17a/f. Expression of these microRNAs was specific for Th17 as compared to other CD4(+) T cell subsets and this was equally valid for in vitro polarized and ex vivo derived cells. From all factors analyzed, IL-23 was the most important cytokine for the in vitro induction of miR-133b and miR-206 in naive CD4(+) T cells of wild type mice. However, analysis of IL-23R deficient mice revealed that IL-23R signaling was not essential for the induction of miR-133b and miR-206. Importantly, we found a similar co-regulation in CCR6(+) and other γδ T cell subsets that are predisposed to production of IL-17. Taken together, we discovered a novel feature of T cell differentiation towards an IL-17-producing phenotype that is shared between αβ and γδ T cells. Notably, the specific co-regulation of miR-133b and miR-206 with the Il17a/f locus also extended to human Th17 cells. This qualifies expression of miR-133b and miR-206 in T cells as novel biomarkers for Th17-type immune reactions.

AB - Differentiation of T helper 17 cells (Th17) is a multistep process that involves the cytokines IL-6, TGF-β, and IL-23 as well as IL-1β, IL-21, and TNF-α. Thereby, robust induction of the capacity to produce IL-17 involves epigenetic modifications of the syntenic Il17a/f locus. Using inbred mouse strains, we identified co-regulation of gene transcription at the Il17a/f locus with the nearby microRNAs miR-133b and miR-206 that are clustered approximately 45 kb upstream of Il17a/f. Expression of these microRNAs was specific for Th17 as compared to other CD4(+) T cell subsets and this was equally valid for in vitro polarized and ex vivo derived cells. From all factors analyzed, IL-23 was the most important cytokine for the in vitro induction of miR-133b and miR-206 in naive CD4(+) T cells of wild type mice. However, analysis of IL-23R deficient mice revealed that IL-23R signaling was not essential for the induction of miR-133b and miR-206. Importantly, we found a similar co-regulation in CCR6(+) and other γδ T cell subsets that are predisposed to production of IL-17. Taken together, we discovered a novel feature of T cell differentiation towards an IL-17-producing phenotype that is shared between αβ and γδ T cells. Notably, the specific co-regulation of miR-133b and miR-206 with the Il17a/f locus also extended to human Th17 cells. This qualifies expression of miR-133b and miR-206 in T cells as novel biomarkers for Th17-type immune reactions.

KW - Animals

KW - Base Sequence

KW - Cell Differentiation/genetics

KW - Cell Polarity/genetics

KW - Cells, Cultured

KW - Gene Expression Regulation

KW - Genetic Loci

KW - Humans

KW - Interleukin-17/biosynthesis

KW - Interleukin-23/metabolism

KW - Mice

KW - MicroRNAs/genetics

KW - Molecular Sequence Data

KW - Receptors, Antigen, T-Cell, alpha-beta/metabolism

KW - Receptors, Antigen, T-Cell, gamma-delta/metabolism

KW - Synteny/genetics

KW - Th17 Cells/cytology

U2 - 10.1371/journal.pone.0020171

DO - 10.1371/journal.pone.0020171

M3 - SCORING: Journal article

C2 - 21637854

VL - 6

SP - e20171

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 5

ER -