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Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element.

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Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element. / Prechtel, Alexander T; Chemnitz, Jan; Schirmer, Susann; Ehlers, Christina; Langbein-Detsch, Ines; Stülke, Jörg; Dabauvalle, Marie-Christine; Kehlenbach, Ralph H; Hauber, Joachim.

in: J BIOL CHEM, Jahrgang 281, Nr. 16, 16, 2006, S. 10912-10925.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Prechtel, AT, Chemnitz, J, Schirmer, S, Ehlers, C, Langbein-Detsch, I, Stülke, J, Dabauvalle, M-C, Kehlenbach, RH & Hauber, J 2006, 'Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element.', J BIOL CHEM, Jg. 281, Nr. 16, 16, S. 10912-10925. <http://www.ncbi.nlm.nih.gov/pubmed/16484227?dopt=Citation>

APA

Prechtel, A. T., Chemnitz, J., Schirmer, S., Ehlers, C., Langbein-Detsch, I., Stülke, J., Dabauvalle, M-C., Kehlenbach, R. H., & Hauber, J. (2006). Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element. J BIOL CHEM, 281(16), 10912-10925. [16]. http://www.ncbi.nlm.nih.gov/pubmed/16484227?dopt=Citation

Vancouver

Prechtel AT, Chemnitz J, Schirmer S, Ehlers C, Langbein-Detsch I, Stülke J et al. Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element. J BIOL CHEM. 2006;281(16):10912-10925. 16.

Bibtex

@article{383ebe1ba4c34578b620d37a07946bbf,
title = "Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element.",
abstract = "Dendritic cells are the most potent of the antigen-presenting cells and are characterized by surface expression of CD83. Here, we show that the coding region of CD83 mRNA contains a novel cis-acting structured RNA element that binds to HuR, a member of the ELAV family of AU-rich element RNA-binding proteins. Transient transfection of mammalian cells demonstrated that this CD83 mRNA-derived element acts as a post-transcriptional regulatory element in cells overexpressing HuR. Notably, binding of HuR to the CD83 post-transcriptional regulatory element did not affect mRNA stability. Using RNA interference, we show that HuR mediated efficient expression of CD83. In particular, HuR was required for cytoplasmic accumulation of CD83 transcripts. Likewise, inhibition of the CRM1 nuclear export pathway by leptomycin B or overexpression of a defective form of the nucleoporin Nup214/CAN diminished cytoplasmic CD83 mRNA levels. In summary, the data presented demonstrate that the HuR-CRM1 axis affects the nucleocytoplasmic translocation of CD83 mRNA under regular physiological conditions.",
author = "Prechtel, {Alexander T} and Jan Chemnitz and Susann Schirmer and Christina Ehlers and Ines Langbein-Detsch and J{\"o}rg St{\"u}lke and Marie-Christine Dabauvalle and Kehlenbach, {Ralph H} and Joachim Hauber",
year = "2006",
language = "Deutsch",
volume = "281",
pages = "10912--10925",
journal = "J BIOL CHEM",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "16",

}

RIS

TY - JOUR

T1 - Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element.

AU - Prechtel, Alexander T

AU - Chemnitz, Jan

AU - Schirmer, Susann

AU - Ehlers, Christina

AU - Langbein-Detsch, Ines

AU - Stülke, Jörg

AU - Dabauvalle, Marie-Christine

AU - Kehlenbach, Ralph H

AU - Hauber, Joachim

PY - 2006

Y1 - 2006

N2 - Dendritic cells are the most potent of the antigen-presenting cells and are characterized by surface expression of CD83. Here, we show that the coding region of CD83 mRNA contains a novel cis-acting structured RNA element that binds to HuR, a member of the ELAV family of AU-rich element RNA-binding proteins. Transient transfection of mammalian cells demonstrated that this CD83 mRNA-derived element acts as a post-transcriptional regulatory element in cells overexpressing HuR. Notably, binding of HuR to the CD83 post-transcriptional regulatory element did not affect mRNA stability. Using RNA interference, we show that HuR mediated efficient expression of CD83. In particular, HuR was required for cytoplasmic accumulation of CD83 transcripts. Likewise, inhibition of the CRM1 nuclear export pathway by leptomycin B or overexpression of a defective form of the nucleoporin Nup214/CAN diminished cytoplasmic CD83 mRNA levels. In summary, the data presented demonstrate that the HuR-CRM1 axis affects the nucleocytoplasmic translocation of CD83 mRNA under regular physiological conditions.

AB - Dendritic cells are the most potent of the antigen-presenting cells and are characterized by surface expression of CD83. Here, we show that the coding region of CD83 mRNA contains a novel cis-acting structured RNA element that binds to HuR, a member of the ELAV family of AU-rich element RNA-binding proteins. Transient transfection of mammalian cells demonstrated that this CD83 mRNA-derived element acts as a post-transcriptional regulatory element in cells overexpressing HuR. Notably, binding of HuR to the CD83 post-transcriptional regulatory element did not affect mRNA stability. Using RNA interference, we show that HuR mediated efficient expression of CD83. In particular, HuR was required for cytoplasmic accumulation of CD83 transcripts. Likewise, inhibition of the CRM1 nuclear export pathway by leptomycin B or overexpression of a defective form of the nucleoporin Nup214/CAN diminished cytoplasmic CD83 mRNA levels. In summary, the data presented demonstrate that the HuR-CRM1 axis affects the nucleocytoplasmic translocation of CD83 mRNA under regular physiological conditions.

M3 - SCORING: Zeitschriftenaufsatz

VL - 281

SP - 10912

EP - 10925

JO - J BIOL CHEM

JF - J BIOL CHEM

SN - 0021-9258

IS - 16

M1 - 16

ER -