Expression and subcellular localization of Spred proteins in mouse and human tissues
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Expression and subcellular localization of Spred proteins in mouse and human tissues. / Engelhardt, Catherine M; Bundschu, Karin; Messerschmitt, Marlies; Renné, Thomas; Walter, Ulrich; Reinhard, Matthias; Schuh, Kai.
in: HISTOCHEM CELL BIOL, Jahrgang 122, Nr. 6, 01.12.2004, S. 527-38.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Expression and subcellular localization of Spred proteins in mouse and human tissues
AU - Engelhardt, Catherine M
AU - Bundschu, Karin
AU - Messerschmitt, Marlies
AU - Renné, Thomas
AU - Walter, Ulrich
AU - Reinhard, Matthias
AU - Schuh, Kai
PY - 2004/12/1
Y1 - 2004/12/1
N2 - Spred-1 and Spred-2 (Sprouty-related protein with an EVH1 domain) are recently described members of the EVH1 (Ena/VASP-homology domain 1) family. Both Spred-1 and Spred-2 are membrane-associated substrates of receptor tyrosine kinases and they act as negative regulators of the Ras pathway upon growth factor stimulation. Since the Spred family members seem to exert overlapping molecular functions, the isotype-specific function of each member remains enigmatic. To date, no comprehensive expression profiling of Spred proteins has been shown. Therefore, we compared mRNA and protein expression patterns of Spred-1 and Spred-2 systematically in mouse organs. Furthermore, we focused on the tissue-specific expression of Spred-2 in adult human tissues, the subcellular localization, and the potential role of Spred-2 in the organism. Our studies show that expression patterns of Spred-1 and Spred-2 differ markedly among various tissues and cell types. In mouse, Spred-1 and Spred-2 were found to be expressed predominantly in brain, whereas Spred-2 was found to be more widely expressed in various adult tissues than Spred-1. In humans, Spred-2 was found to be strongly expressed in glandular epithelia and, at the subcellular level, its immunoreactivity was associated with secretory vesicles. Using confocal microscopy we found Spred-2 to be strongly colocalized with Rab11 and, to a lesser extent, with Rab5a GTPase, an observation that was not made for Spred-1. We conclude that the two members of the recently discovered Spred protein family, Spred-1 and Spred-2, show a highly specific expression pattern in various tissues reflecting a specific physiological role for the individual Spred isoforms in these tissues. Furthermore, it becomes most likely that Spred-2 is involved in the regulation of secretory pathways.
AB - Spred-1 and Spred-2 (Sprouty-related protein with an EVH1 domain) are recently described members of the EVH1 (Ena/VASP-homology domain 1) family. Both Spred-1 and Spred-2 are membrane-associated substrates of receptor tyrosine kinases and they act as negative regulators of the Ras pathway upon growth factor stimulation. Since the Spred family members seem to exert overlapping molecular functions, the isotype-specific function of each member remains enigmatic. To date, no comprehensive expression profiling of Spred proteins has been shown. Therefore, we compared mRNA and protein expression patterns of Spred-1 and Spred-2 systematically in mouse organs. Furthermore, we focused on the tissue-specific expression of Spred-2 in adult human tissues, the subcellular localization, and the potential role of Spred-2 in the organism. Our studies show that expression patterns of Spred-1 and Spred-2 differ markedly among various tissues and cell types. In mouse, Spred-1 and Spred-2 were found to be expressed predominantly in brain, whereas Spred-2 was found to be more widely expressed in various adult tissues than Spred-1. In humans, Spred-2 was found to be strongly expressed in glandular epithelia and, at the subcellular level, its immunoreactivity was associated with secretory vesicles. Using confocal microscopy we found Spred-2 to be strongly colocalized with Rab11 and, to a lesser extent, with Rab5a GTPase, an observation that was not made for Spred-1. We conclude that the two members of the recently discovered Spred protein family, Spred-1 and Spred-2, show a highly specific expression pattern in various tissues reflecting a specific physiological role for the individual Spred isoforms in these tissues. Furthermore, it becomes most likely that Spred-2 is involved in the regulation of secretory pathways.
KW - Animals
KW - Antibody Formation
KW - Antibody Specificity
KW - Brain
KW - Decidua
KW - Female
KW - Gene Expression Profiling
KW - Gene Expression Regulation, Developmental
KW - Humans
KW - Liver
KW - Lung
KW - MAP Kinase Signaling System
KW - Male
KW - Mice
KW - Microscopy, Confocal
KW - Myocardium
KW - Pregnancy
KW - Repressor Proteins
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Testis
KW - rab GTP-Binding Proteins
U2 - 10.1007/s00418-004-0725-6
DO - 10.1007/s00418-004-0725-6
M3 - SCORING: Journal article
C2 - 15580519
VL - 122
SP - 527
EP - 538
JO - HISTOCHEM CELL BIOL
JF - HISTOCHEM CELL BIOL
SN - 0948-6143
IS - 6
ER -