Experimental epilepsy affects Notch1 signalling and the stem cell pool in the dentate gyrus.
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Experimental epilepsy affects Notch1 signalling and the stem cell pool in the dentate gyrus. / Sibbe, Mirjam; Häussler, Ute; Dieni, Sandra; Althof, Daniel; Haas, Carola A; Frotscher, Michael.
in: EUR J NEUROSCI, Jahrgang 36, Nr. 12, 12, 2012, S. 3643-3652.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Experimental epilepsy affects Notch1 signalling and the stem cell pool in the dentate gyrus.
AU - Sibbe, Mirjam
AU - Häussler, Ute
AU - Dieni, Sandra
AU - Althof, Daniel
AU - Haas, Carola A
AU - Frotscher, Michael
PY - 2012
Y1 - 2012
N2 - Temporal lobe epilepsy (TLE) is the most frequent form of epilepsy in adults. In addition to recurrent focal seizures, patients suffer from memory loss and depression. The factors contributing to these symptoms are unknown. In recent years, adult hippocampal neurogenesis has been implicated in certain aspects of learning and memory, as well as in depression and anhedonia. Here we investigated whether the adult hippocampal stem cell niche is affected by status epilepticus in a mouse model of TLE using unilateral intrahippocampal kainic acid injection. Eight days after status epilepticus, we found a strong diminution in Notch signalling, a key pathway involved in stem cell maintenance, as assayed by hes5 reporter gene activity. In particular, hes5-GFP expression in the subgranular zone of the dentate gyrus was diminished. Furthermore, Sox2-positive cells as well as stem cell proliferation were reduced, thus pointing to a disruption of the stem cell niche in epilepsy under the present experimental conditions.
AB - Temporal lobe epilepsy (TLE) is the most frequent form of epilepsy in adults. In addition to recurrent focal seizures, patients suffer from memory loss and depression. The factors contributing to these symptoms are unknown. In recent years, adult hippocampal neurogenesis has been implicated in certain aspects of learning and memory, as well as in depression and anhedonia. Here we investigated whether the adult hippocampal stem cell niche is affected by status epilepticus in a mouse model of TLE using unilateral intrahippocampal kainic acid injection. Eight days after status epilepticus, we found a strong diminution in Notch signalling, a key pathway involved in stem cell maintenance, as assayed by hes5 reporter gene activity. In particular, hes5-GFP expression in the subgranular zone of the dentate gyrus was diminished. Furthermore, Sox2-positive cells as well as stem cell proliferation were reduced, thus pointing to a disruption of the stem cell niche in epilepsy under the present experimental conditions.
KW - Animals
KW - Male
KW - Disease Models, Animal
KW - Mice
KW - Mice, Inbred C57BL
KW - Gene Expression
KW - Cell Proliferation
KW - Genes, Reporter
KW - Signal Transduction
KW - Basic Helix-Loop-Helix Transcription Factors/genetics/metabolism
KW - Stem Cell Niche
KW - Repressor Proteins/genetics/metabolism
KW - Adult Stem Cells/metabolism
KW - Dentate Gyrus/pathology
KW - Epilepsy, Temporal Lobe/chemically induced/metabolism/pathology
KW - Kainic Acid
KW - Receptor, Notch1/metabolism
KW - SOXB1 Transcription Factors/genetics/metabolism
KW - Status Epilepticus/chemically induced/metabolism/pathology
KW - Animals
KW - Male
KW - Disease Models, Animal
KW - Mice
KW - Mice, Inbred C57BL
KW - Gene Expression
KW - Cell Proliferation
KW - Genes, Reporter
KW - Signal Transduction
KW - Basic Helix-Loop-Helix Transcription Factors/genetics/metabolism
KW - Stem Cell Niche
KW - Repressor Proteins/genetics/metabolism
KW - Adult Stem Cells/metabolism
KW - Dentate Gyrus/pathology
KW - Epilepsy, Temporal Lobe/chemically induced/metabolism/pathology
KW - Kainic Acid
KW - Receptor, Notch1/metabolism
KW - SOXB1 Transcription Factors/genetics/metabolism
KW - Status Epilepticus/chemically induced/metabolism/pathology
M3 - SCORING: Journal article
VL - 36
SP - 3643
EP - 3652
JO - EUR J NEUROSCI
JF - EUR J NEUROSCI
SN - 0953-816X
IS - 12
M1 - 12
ER -
