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Ex vivo cytosolic delivery of functional macromolecules to immune cells

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Ex vivo cytosolic delivery of functional macromolecules to immune cells. / Sharei, Armon; Trifonova, Radiana; Jhunjhunwala, Siddharth; Hartoularos, George C; Eyerman, Alexandra T; Lytton-Jean, Abigail; Angin, Mathieu; Sharma, Siddhartha; Poceviciute, Roberta; Mao, Shirley; Heimann, Megan; Liu, Sophia; Talkar, Tanya; Khan, Omar F; Addo, Marylyn Martina; von Andrian, Ulrich H; Anderson, Daniel G; Langer, Robert; Lieberman, Judy; Jensen, Klavs F.

in: PLOS ONE, Jahrgang 10, Nr. 4, 2015, S. e0118803.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Sharei, A, Trifonova, R, Jhunjhunwala, S, Hartoularos, GC, Eyerman, AT, Lytton-Jean, A, Angin, M, Sharma, S, Poceviciute, R, Mao, S, Heimann, M, Liu, S, Talkar, T, Khan, OF, Addo, MM, von Andrian, UH, Anderson, DG, Langer, R, Lieberman, J & Jensen, KF 2015, 'Ex vivo cytosolic delivery of functional macromolecules to immune cells', PLOS ONE, Jg. 10, Nr. 4, S. e0118803. https://doi.org/10.1371/journal.pone.0118803

APA

Sharei, A., Trifonova, R., Jhunjhunwala, S., Hartoularos, G. C., Eyerman, A. T., Lytton-Jean, A., Angin, M., Sharma, S., Poceviciute, R., Mao, S., Heimann, M., Liu, S., Talkar, T., Khan, O. F., Addo, M. M., von Andrian, U. H., Anderson, D. G., Langer, R., Lieberman, J., & Jensen, K. F. (2015). Ex vivo cytosolic delivery of functional macromolecules to immune cells. PLOS ONE, 10(4), e0118803. https://doi.org/10.1371/journal.pone.0118803

Vancouver

Sharei A, Trifonova R, Jhunjhunwala S, Hartoularos GC, Eyerman AT, Lytton-Jean A et al. Ex vivo cytosolic delivery of functional macromolecules to immune cells. PLOS ONE. 2015;10(4):e0118803. https://doi.org/10.1371/journal.pone.0118803

Bibtex

@article{f490b348dca3492981457be328d0bf59,
title = "Ex vivo cytosolic delivery of functional macromolecules to immune cells",
abstract = "Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach's potential to engineer cell function is demonstrated in HIV infection studies.",
author = "Armon Sharei and Radiana Trifonova and Siddharth Jhunjhunwala and Hartoularos, {George C} and Eyerman, {Alexandra T} and Abigail Lytton-Jean and Mathieu Angin and Siddhartha Sharma and Roberta Poceviciute and Shirley Mao and Megan Heimann and Sophia Liu and Tanya Talkar and Khan, {Omar F} and Addo, {Marylyn Martina} and {von Andrian}, {Ulrich H} and Anderson, {Daniel G} and Robert Langer and Judy Lieberman and Jensen, {Klavs F}",
year = "2015",
doi = "10.1371/journal.pone.0118803",
language = "English",
volume = "10",
pages = "e0118803",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "4",

}

RIS

TY - JOUR

T1 - Ex vivo cytosolic delivery of functional macromolecules to immune cells

AU - Sharei, Armon

AU - Trifonova, Radiana

AU - Jhunjhunwala, Siddharth

AU - Hartoularos, George C

AU - Eyerman, Alexandra T

AU - Lytton-Jean, Abigail

AU - Angin, Mathieu

AU - Sharma, Siddhartha

AU - Poceviciute, Roberta

AU - Mao, Shirley

AU - Heimann, Megan

AU - Liu, Sophia

AU - Talkar, Tanya

AU - Khan, Omar F

AU - Addo, Marylyn Martina

AU - von Andrian, Ulrich H

AU - Anderson, Daniel G

AU - Langer, Robert

AU - Lieberman, Judy

AU - Jensen, Klavs F

PY - 2015

Y1 - 2015

N2 - Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach's potential to engineer cell function is demonstrated in HIV infection studies.

AB - Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach's potential to engineer cell function is demonstrated in HIV infection studies.

U2 - 10.1371/journal.pone.0118803

DO - 10.1371/journal.pone.0118803

M3 - SCORING: Journal article

C2 - 25875117

VL - 10

SP - e0118803

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 4

ER -