Ex vivo aorta patch model for analysis of cellular adhesion

Yuan-Na Lin; Raymond Nqobizitha Thata; Antonio Virgilio Failla; Markus Geissen; Guenter Daum; Sabine Windhorst

doi:10.1016/j.tice.2015.03.004

Ex vivo aorta patch model for analysis of cellular adhesion

Standard

Ex vivo aorta patch model for analysis of cellular adhesion. / Lin, Yuan-Na; Thata, Raymond Nqobizitha; Failla, Antonio Virgilio ; Geissen, Markus; Daum, Guenter; Windhorst, Sabine.

in: TISSUE CELL, Jahrgang 47, Nr. 3, 06.2015, S. 266-272.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Lin, Y-N, Thata, RN, Failla, AV , Geissen, M, Daum, G & Windhorst, S 2015, 'Ex vivo aorta patch model for analysis of cellular adhesion', TISSUE CELL, Jg. 47, Nr. 3, S. 266-272. https://doi.org/10.1016/j.tice.2015.03.004

APA

Lin, Y-N., Thata, R. N., Failla, A. V., Geissen, M., Daum, G., & Windhorst, S. (2015). Ex vivo aorta patch model for analysis of cellular adhesion. TISSUE CELL, 47(3), 266-272. https://doi.org/10.1016/j.tice.2015.03.004

Vancouver

Lin Y-N, Thata RN, Failla AV , Geissen M, Daum G, Windhorst S. Ex vivo aorta patch model for analysis of cellular adhesion. TISSUE CELL. 2015 Jun;47(3):266-272. https://doi.org/10.1016/j.tice.2015.03.004

Bibtex

@article{6e6051201a72446eba02aac741113c78,

title = "Ex vivo aorta patch model for analysis of cellular adhesion",

abstract = "The vascular endothelium as well as subendothelium are objects of many researches as it is directly involved in a multiplicity of physiological and pathological settings. Detailed study of endothelial function became feasible with the development of techniques to culture endothelial cells (EC) in vitro. Limitations of this approach have become apparent with the realization that cell culture dedifferentiate with time and do not exhibit properties of intact tissue. Here we describe the development of a novel ex vivo tissue model to study cell-vascular wall interactions by using isolated mouse aorta patches. Validation of this model was performed by demonstrating cell attachment and changes in cell shape typical for cell spreading during adhesion. A major advantage of this model is that cell-endothelium interaction and its molecular backgrounds can now be studied more feasibly on an intact and native tissue.",

author = "Yuan-Na Lin and Thata, {Raymond Nqobizitha} and Failla, {Antonio Virgilio} and Markus Geissen and Guenter Daum and Sabine Windhorst",

year = "2015",

month = jun,

doi = "10.1016/j.tice.2015.03.004",

language = "English",

volume = "47",

pages = "266--272",

journal = "TISSUE CELL",

issn = "0040-8166",

publisher = "Churchill Livingstone",

number = "3",

}

RIS

TY - JOUR

T1 - Ex vivo aorta patch model for analysis of cellular adhesion

AU - Lin, Yuan-Na

AU - Thata, Raymond Nqobizitha

AU - Failla, Antonio Virgilio

AU - Geissen, Markus

AU - Daum, Guenter

AU - Windhorst, Sabine

PY - 2015/6

Y1 - 2015/6

N2 - The vascular endothelium as well as subendothelium are objects of many researches as it is directly involved in a multiplicity of physiological and pathological settings. Detailed study of endothelial function became feasible with the development of techniques to culture endothelial cells (EC) in vitro. Limitations of this approach have become apparent with the realization that cell culture dedifferentiate with time and do not exhibit properties of intact tissue. Here we describe the development of a novel ex vivo tissue model to study cell-vascular wall interactions by using isolated mouse aorta patches. Validation of this model was performed by demonstrating cell attachment and changes in cell shape typical for cell spreading during adhesion. A major advantage of this model is that cell-endothelium interaction and its molecular backgrounds can now be studied more feasibly on an intact and native tissue.

AB - The vascular endothelium as well as subendothelium are objects of many researches as it is directly involved in a multiplicity of physiological and pathological settings. Detailed study of endothelial function became feasible with the development of techniques to culture endothelial cells (EC) in vitro. Limitations of this approach have become apparent with the realization that cell culture dedifferentiate with time and do not exhibit properties of intact tissue. Here we describe the development of a novel ex vivo tissue model to study cell-vascular wall interactions by using isolated mouse aorta patches. Validation of this model was performed by demonstrating cell attachment and changes in cell shape typical for cell spreading during adhesion. A major advantage of this model is that cell-endothelium interaction and its molecular backgrounds can now be studied more feasibly on an intact and native tissue.

U2 - 10.1016/j.tice.2015.03.004

DO - 10.1016/j.tice.2015.03.004

M3 - SCORING: Journal article

C2 - 25890870

VL - 47

SP - 266

EP - 272

JO - TISSUE CELL

JF - TISSUE CELL

SN - 0040-8166

IS - 3

ER -