Evolution and transmission of stable CTL escape mutations in HIV infection
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Evolution and transmission of stable CTL escape mutations in HIV infection. / Goulder, P J; Brander, C; Tang, Y; Tremblay, C; Colbert, R A; Addo, M M; Rosenberg, E S; Nguyen, T; Allen, R; Trocha, A; Altfeld, M; He, S; Bunce, M; Funkhouser, R; Pelton, S I; Burchett, S K; McIntosh, K; Korber, B T; Walker, B D.
in: NATURE, Jahrgang 412, Nr. 6844, 19.07.2001, S. 334-8.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Evolution and transmission of stable CTL escape mutations in HIV infection
AU - Goulder, P J
AU - Brander, C
AU - Tang, Y
AU - Tremblay, C
AU - Colbert, R A
AU - Addo, M M
AU - Rosenberg, E S
AU - Nguyen, T
AU - Allen, R
AU - Trocha, A
AU - Altfeld, M
AU - He, S
AU - Bunce, M
AU - Funkhouser, R
AU - Pelton, S I
AU - Burchett, S K
AU - McIntosh, K
AU - Korber, B T
AU - Walker, B D
PY - 2001/7/19
Y1 - 2001/7/19
N2 - Increasing evidence indicates that potent anti-HIV-1 activity is mediated by cytotoxic T lymphocytes (CTLs); however, the effects of this immune pressure on viral transmission and evolution have not been determined. Here we investigate mother-child transmission in the setting of human leukocyte antigen (HLA)-B27 expression, selected for analysis because it is associated with prolonged immune containment in adult infection. In adults, mutations in a dominant and highly conserved B27-restricted Gag CTL epitope lead to loss of recognition and disease progression. In mothers expressing HLA-B27 who transmit HIV-1 perinatally, we document transmission of viruses encoding CTL escape variants in this dominant Gag epitope that no longer bind to B27. Their infected infants target an otherwise subdominant B27-restricted epitope and fail to contain HIV replication. These CTL escape variants remain stable without reversion in the absence of the evolutionary pressure that originally selected the mutation. These data suggest that CTL escape mutations in epitopes associated with suppression of viraemia will accumulate as the epidemic progresses, and therefore have important implications for vaccine design.
AB - Increasing evidence indicates that potent anti-HIV-1 activity is mediated by cytotoxic T lymphocytes (CTLs); however, the effects of this immune pressure on viral transmission and evolution have not been determined. Here we investigate mother-child transmission in the setting of human leukocyte antigen (HLA)-B27 expression, selected for analysis because it is associated with prolonged immune containment in adult infection. In adults, mutations in a dominant and highly conserved B27-restricted Gag CTL epitope lead to loss of recognition and disease progression. In mothers expressing HLA-B27 who transmit HIV-1 perinatally, we document transmission of viruses encoding CTL escape variants in this dominant Gag epitope that no longer bind to B27. Their infected infants target an otherwise subdominant B27-restricted epitope and fail to contain HIV replication. These CTL escape variants remain stable without reversion in the absence of the evolutionary pressure that originally selected the mutation. These data suggest that CTL escape mutations in epitopes associated with suppression of viraemia will accumulate as the epidemic progresses, and therefore have important implications for vaccine design.
KW - Adult
KW - Child
KW - DNA, Viral
KW - Disease Progression
KW - Epitopes, T-Lymphocyte/genetics
KW - Female
KW - HIV Infections/genetics
KW - HIV-1/genetics
KW - HLA-B27 Antigen/immunology
KW - Histocompatibility Testing
KW - Humans
KW - Infectious Disease Transmission, Vertical
KW - Mutation
KW - T-Lymphocytes, Cytotoxic/immunology
U2 - 10.1038/35085576
DO - 10.1038/35085576
M3 - SCORING: Journal article
C2 - 11460164
VL - 412
SP - 334
EP - 338
JO - NATURE
JF - NATURE
SN - 0028-0836
IS - 6844
ER -