Evaluation of P2X7 Receptor Function in Tumor Contexts Using rAAV Vector and Nanobodies (AAVnano)
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Evaluation of P2X7 Receptor Function in Tumor Contexts Using rAAV Vector and Nanobodies (AAVnano). / Demeules, Mélanie; Scarpitta, Allan; Abad, Catalina; Gondé, Henri; Hardet, Romain; Pinto-Espinoza, Carolina; Eichhoff, Anna Marei; Schäfer, Waldemar; Haag, Friedrich; Koch-Nolte, Friedrich; Adriouch, Sahil.
in: FRONT ONCOL, Jahrgang 10, 1699, 2020.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Evaluation of P2X7 Receptor Function in Tumor Contexts Using rAAV Vector and Nanobodies (AAVnano)
AU - Demeules, Mélanie
AU - Scarpitta, Allan
AU - Abad, Catalina
AU - Gondé, Henri
AU - Hardet, Romain
AU - Pinto-Espinoza, Carolina
AU - Eichhoff, Anna Marei
AU - Schäfer, Waldemar
AU - Haag, Friedrich
AU - Koch-Nolte, Friedrich
AU - Adriouch, Sahil
N1 - Copyright © 2020 Demeules, Scarpitta, Abad, Gondé, Hardet, Pinto-Espinoza, Eichhoff, Schäfer, Haag, Koch-Nolte and Adriouch.
PY - 2020
Y1 - 2020
N2 - Adenosine triphosphate (ATP) represents a danger signal that accumulates in injured tissues, in inflammatory sites, and in the tumor microenvironment. Extracellular ATP is known to signal through plasma membrane receptors of the P2Y and P2X families. Among the P2X receptors, P2X7 has attracted increasing interest in the field of inflammation as well as in cancer. P2X7 is expressed by immune cells and by most malignant tumor cells where it plays a crucial yet complex role that remains to be clarified. P2X7 activity has been associated with production and release of pro-inflammatory cytokines, modulation of the activity and survival of immune cells, and the stimulation of proliferation and migratory properties of tumor cells. Hence, P2X7 plays an intricate role in the tumor microenvironment combining beneficial and detrimental effects that need to be further investigated. For this, we developed a novel methodology termed AAVnano based on the use of Adeno-associated viral vectors (AAV) encoding nanobodies targeting P2X7. We discuss here the advantages of this tool to study the different functions of P2X7 in cancer and other pathophysiological contexts.
AB - Adenosine triphosphate (ATP) represents a danger signal that accumulates in injured tissues, in inflammatory sites, and in the tumor microenvironment. Extracellular ATP is known to signal through plasma membrane receptors of the P2Y and P2X families. Among the P2X receptors, P2X7 has attracted increasing interest in the field of inflammation as well as in cancer. P2X7 is expressed by immune cells and by most malignant tumor cells where it plays a crucial yet complex role that remains to be clarified. P2X7 activity has been associated with production and release of pro-inflammatory cytokines, modulation of the activity and survival of immune cells, and the stimulation of proliferation and migratory properties of tumor cells. Hence, P2X7 plays an intricate role in the tumor microenvironment combining beneficial and detrimental effects that need to be further investigated. For this, we developed a novel methodology termed AAVnano based on the use of Adeno-associated viral vectors (AAV) encoding nanobodies targeting P2X7. We discuss here the advantages of this tool to study the different functions of P2X7 in cancer and other pathophysiological contexts.
U2 - 10.3389/fonc.2020.01699
DO - 10.3389/fonc.2020.01699
M3 - SCORING: Journal article
C2 - 33042812
VL - 10
JO - FRONT ONCOL
JF - FRONT ONCOL
SN - 2234-943X
M1 - 1699
ER -
