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Evaluation of different detection methods of biofilm formation in Staphylococcus aureus.

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Evaluation of different detection methods of biofilm formation in Staphylococcus aureus. / Knobloch, Johannes K-M; Horstkotte, Matthias A; Rohde, Holger; Mack, Dietrich.

in: MED MICROBIOL IMMUN, Jahrgang 191, Nr. 2, 2, 2002, S. 101-106.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Knobloch, JK-M, Horstkotte, MA, Rohde, H & Mack, D 2002, 'Evaluation of different detection methods of biofilm formation in Staphylococcus aureus.', MED MICROBIOL IMMUN, Jg. 191, Nr. 2, 2, S. 101-106. https://doi.org/10.1007/s00430-002-0124-3

APA

Knobloch, J. K-M., Horstkotte, M. A., Rohde, H., & Mack, D. (2002). Evaluation of different detection methods of biofilm formation in Staphylococcus aureus. MED MICROBIOL IMMUN, 191(2), 101-106. [2]. https://doi.org/10.1007/s00430-002-0124-3

Vancouver

Knobloch JK-M, Horstkotte MA, Rohde H, Mack D. Evaluation of different detection methods of biofilm formation in Staphylococcus aureus. MED MICROBIOL IMMUN. 2002;191(2):101-106. 2. https://doi.org/10.1007/s00430-002-0124-3

Bibtex

@article{62efa0379396458daf93a85af83f8dd1,
title = "Evaluation of different detection methods of biofilm formation in Staphylococcus aureus.",
abstract = "The icaADBC gene locus of Staphylococcus aureus and its polysaccharide intercellular adhesin (PIA/PNSG) were recently identified, but biofilm formation has rarely been detected in vitro. In this study we evaluated a tissue culture plate (TCP) assay and a tube test, as well as Congo red agar, using the two basic media trypticase soy broth (TSB) and brain heart infusion (BHI) broth with different sugar supplements for detection of biofilm formation in 128 ica-positive S. aureus isolates. Of the S. aureus strains, 57.1% displayed a biofilm-positive phenotype under optimized conditions in the TCP test. The tube test correlated well with the TCP test for strongly biofilm-producing strains, whereas weak producers were not safely discriminated from biofilm-negative strains. Screening on Congo red agar displayed a strong correlation with the TCP and the tube test for only 3.8%, and is therefore not recommended for investigation of biofilm formation in S. aureus.",
author = "Knobloch, {Johannes K-M} and Horstkotte, {Matthias A} and Holger Rohde and Dietrich Mack",
year = "2002",
doi = "10.1007/s00430-002-0124-3",
language = "Deutsch",
volume = "191",
pages = "101--106",
journal = "MED MICROBIOL IMMUN",
issn = "0300-8584",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Evaluation of different detection methods of biofilm formation in Staphylococcus aureus.

AU - Knobloch, Johannes K-M

AU - Horstkotte, Matthias A

AU - Rohde, Holger

AU - Mack, Dietrich

PY - 2002

Y1 - 2002

N2 - The icaADBC gene locus of Staphylococcus aureus and its polysaccharide intercellular adhesin (PIA/PNSG) were recently identified, but biofilm formation has rarely been detected in vitro. In this study we evaluated a tissue culture plate (TCP) assay and a tube test, as well as Congo red agar, using the two basic media trypticase soy broth (TSB) and brain heart infusion (BHI) broth with different sugar supplements for detection of biofilm formation in 128 ica-positive S. aureus isolates. Of the S. aureus strains, 57.1% displayed a biofilm-positive phenotype under optimized conditions in the TCP test. The tube test correlated well with the TCP test for strongly biofilm-producing strains, whereas weak producers were not safely discriminated from biofilm-negative strains. Screening on Congo red agar displayed a strong correlation with the TCP and the tube test for only 3.8%, and is therefore not recommended for investigation of biofilm formation in S. aureus.

AB - The icaADBC gene locus of Staphylococcus aureus and its polysaccharide intercellular adhesin (PIA/PNSG) were recently identified, but biofilm formation has rarely been detected in vitro. In this study we evaluated a tissue culture plate (TCP) assay and a tube test, as well as Congo red agar, using the two basic media trypticase soy broth (TSB) and brain heart infusion (BHI) broth with different sugar supplements for detection of biofilm formation in 128 ica-positive S. aureus isolates. Of the S. aureus strains, 57.1% displayed a biofilm-positive phenotype under optimized conditions in the TCP test. The tube test correlated well with the TCP test for strongly biofilm-producing strains, whereas weak producers were not safely discriminated from biofilm-negative strains. Screening on Congo red agar displayed a strong correlation with the TCP and the tube test for only 3.8%, and is therefore not recommended for investigation of biofilm formation in S. aureus.

U2 - 10.1007/s00430-002-0124-3

DO - 10.1007/s00430-002-0124-3

M3 - SCORING: Zeitschriftenaufsatz

VL - 191

SP - 101

EP - 106

JO - MED MICROBIOL IMMUN

JF - MED MICROBIOL IMMUN

SN - 0300-8584

IS - 2

M1 - 2

ER -