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ESR1 amplification in breast cancer by optimized RNase FISH: frequent but low-level and heterogeneous

Standard

ESR1 amplification in breast cancer by optimized RNase FISH: frequent but low-level and heterogeneous. / Moelans, Cathy B; Holst, Frederik; Hellwinkel, Olaf; Simon, Ronald; van Diest, Paul J.

in: PLOS ONE, Jahrgang 8, Nr. 12, 01.01.2013, S. e84189.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Moelans, CB, Holst, F, Hellwinkel, O, Simon, R & van Diest, PJ 2013, 'ESR1 amplification in breast cancer by optimized RNase FISH: frequent but low-level and heterogeneous', PLOS ONE, Jg. 8, Nr. 12, S. e84189. https://doi.org/10.1371/journal.pone.0084189

APA

Moelans, C. B., Holst, F., Hellwinkel, O., Simon, R., & van Diest, P. J. (2013). ESR1 amplification in breast cancer by optimized RNase FISH: frequent but low-level and heterogeneous. PLOS ONE, 8(12), e84189. https://doi.org/10.1371/journal.pone.0084189

Vancouver

Moelans CB, Holst F, Hellwinkel O, Simon R, van Diest PJ. ESR1 amplification in breast cancer by optimized RNase FISH: frequent but low-level and heterogeneous. PLOS ONE. 2013 Jan 1;8(12):e84189. https://doi.org/10.1371/journal.pone.0084189

Bibtex

@article{0afe512b89b6439f9303d3e3b24b7b72,
title = "ESR1 amplification in breast cancer by optimized RNase FISH: frequent but low-level and heterogeneous",
abstract = "Prevalence of ESR1 amplification in breast cancer is highly disputed and discrepancies have been related to different technical protocols and different scoring approaches. In addition, pre-mRNA artifacts have been proposed to influence outcome of ESR1 FISH analysis. We analyzed ESR1 gene copy number status combining an improved RNase FISH protocol with multiplex ligation-dependent probe amplification (MLPA) after laser microdissection. FISH showed a high prevalence of ESR1 gains and amplifications despite RNase treatment but MLPA did not confirm ESR1 copy number increases detected by FISH in more than half of cases. We suggest that the combination of the ESR1-specific intra-tumor heterogeneity and low-level copy number increase accounts for these discrepancies.",
author = "Moelans, {Cathy B} and Frederik Holst and Olaf Hellwinkel and Ronald Simon and {van Diest}, {Paul J}",
year = "2013",
month = jan,
day = "1",
doi = "10.1371/journal.pone.0084189",
language = "English",
volume = "8",
pages = "e84189",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

RIS

TY - JOUR

T1 - ESR1 amplification in breast cancer by optimized RNase FISH: frequent but low-level and heterogeneous

AU - Moelans, Cathy B

AU - Holst, Frederik

AU - Hellwinkel, Olaf

AU - Simon, Ronald

AU - van Diest, Paul J

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Prevalence of ESR1 amplification in breast cancer is highly disputed and discrepancies have been related to different technical protocols and different scoring approaches. In addition, pre-mRNA artifacts have been proposed to influence outcome of ESR1 FISH analysis. We analyzed ESR1 gene copy number status combining an improved RNase FISH protocol with multiplex ligation-dependent probe amplification (MLPA) after laser microdissection. FISH showed a high prevalence of ESR1 gains and amplifications despite RNase treatment but MLPA did not confirm ESR1 copy number increases detected by FISH in more than half of cases. We suggest that the combination of the ESR1-specific intra-tumor heterogeneity and low-level copy number increase accounts for these discrepancies.

AB - Prevalence of ESR1 amplification in breast cancer is highly disputed and discrepancies have been related to different technical protocols and different scoring approaches. In addition, pre-mRNA artifacts have been proposed to influence outcome of ESR1 FISH analysis. We analyzed ESR1 gene copy number status combining an improved RNase FISH protocol with multiplex ligation-dependent probe amplification (MLPA) after laser microdissection. FISH showed a high prevalence of ESR1 gains and amplifications despite RNase treatment but MLPA did not confirm ESR1 copy number increases detected by FISH in more than half of cases. We suggest that the combination of the ESR1-specific intra-tumor heterogeneity and low-level copy number increase accounts for these discrepancies.

U2 - 10.1371/journal.pone.0084189

DO - 10.1371/journal.pone.0084189

M3 - SCORING: Journal article

C2 - 24367641

VL - 8

SP - e84189

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 12

ER -