Endoscopic ICG perfusion imaging for flap transplants: technical development

TY - CHAP

T1 - Endoscopic ICG perfusion imaging for flap transplants: technical development

AU - Stepp, Herbert

AU - Schachenmayr, Hilmar

AU - Ehrhardt, Andre

AU - Goebel, Werner

AU - Zhorzel, Sven

AU - Betz, Christian Stephan

N1 - Conference on Photonic Therapeutics and Diagnostics VI, San Francisco, CA, JAN 23-25, 2010

PY - 2010

Y1 - 2010

N2 - Objective: Following tumour surgery in the head and neck region, skin flap transplants are usually required to cover the resection area. The purpose of the development was to provide a simple and reliable means to assess whether the transplanted flap is sufficiently perfused. Methods: Fluorescence of intravenously injected Indocyanine green (ICG) was detected with a slightly modified 3-chip CCD camera. Appropriately coated optical filters allow for excitation of ICG with NIR light and detection of NIR ICG-fluorescence with the blue channel of the camera. In addition, low intensities of white light can be transmitted to allow for simultaneous display of a remission image in the green and red channels of the camera. Further processing was performed with a LabVIEW program. Results: A satisfactory white light image (red, green and blue display (RGB)) could be calculated from the remission images recorded with the green and red channels of the camera via a look-up table. The look-up table was programmed to provide an optimized blue intensity value for each combination of red and green values. This was generated using a reference image. Implementation of image tracking and intensity measurements in regions of interest (ROIs) in the images is useful to reliably monitor perfusion kinetics of flap and adjacent normal tissue.

AB - Objective: Following tumour surgery in the head and neck region, skin flap transplants are usually required to cover the resection area. The purpose of the development was to provide a simple and reliable means to assess whether the transplanted flap is sufficiently perfused. Methods: Fluorescence of intravenously injected Indocyanine green (ICG) was detected with a slightly modified 3-chip CCD camera. Appropriately coated optical filters allow for excitation of ICG with NIR light and detection of NIR ICG-fluorescence with the blue channel of the camera. In addition, low intensities of white light can be transmitted to allow for simultaneous display of a remission image in the green and red channels of the camera. Further processing was performed with a LabVIEW program. Results: A satisfactory white light image (red, green and blue display (RGB)) could be calculated from the remission images recorded with the green and red channels of the camera via a look-up table. The look-up table was programmed to provide an optimized blue intensity value for each combination of red and green values. This was generated using a reference image. Implementation of image tracking and intensity measurements in regions of interest (ROIs) in the images is useful to reliably monitor perfusion kinetics of flap and adjacent normal tissue.

KW - Indocyanine green

KW - fluorescence imaging

KW - perfusion

KW - skin flap transplants

KW - endoscopy

U2 - 10.1117/12.847857

DO - 10.1117/12.847857

M3 - SCORING: Contribution to collected editions/anthologies

SN - 978-0-8194-7944-0

VL - 7548

T3 - Proceedings of SPIE

BT - PHOTONIC THERAPEUTICS AND DIAGNOSTICS VI

A2 - Kollias, N

A2 - Choi, B

A2 - Zeng, H

A2 - Malek, RS

A2 - Wong, BJF

A2 - IIgner, JFR

A2 - Gregory, KW

A2 - Guillermo, JT

A2 - Marcu, L

A2 - Hirschberg, H

A2 - Madsen, SJ

A2 - Mandelis, A

A2 - MahadevanJansen, A

A2 - ED, Jansen

PB - SPIE-INT SOC OPTICAL ENGINEERING

CY - 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA

ER -