Endocultivation the influence of delayed vs. simultaneous application of BMP-2 onto individually formed hydroxyapatite matrices for heterotopic bone induction

TY - JOUR

T1 - Endocultivation the influence of delayed vs. simultaneous application of BMP-2 onto individually formed hydroxyapatite matrices for heterotopic bone induction

AU - Becker, S T

AU - Bolte, H

AU - Schünemann, K

AU - Seitz, H

AU - Bara, J J

AU - Beck-Broichsitter, B E

AU - Russo, P A J

AU - Wiltfang, J

AU - Warnke, P H

N1 - Copyright © 2012 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

PY - 2012/9/1

Y1 - 2012/9/1

N2 - When bone morphogenetic protein (BMP) is delivered to matrices in vivo may affect tissue engineered bone constructs for jaw reconstruction after cancer surgery. This study compared the effects of BMP application at different times after matrix implantation for heterotopic bone induction in a rat model. Hydroxyapatite blocks were implanted unilaterally onto the surface of the latissimus dorsi muscle. A second block was implanted onto the contralateral muscle after 1, 2 or 4 weeks and 200 μg rhBMP-2 was injected into the blocks on both sides. Bone formation and density inside the blocks was analysed by CT and histology. 8 weeks after BMP application increases in bone density within the scaffolds were most pronounced in the simultaneous application group (179 HU). Less pronounced increases were observed for the 1 (65 HU), 2 (58 HU) and 4 (31 HU; p<0.0001) week delay group. Homogeneous bone induction started from the central channel of the blocks. Capillaries and larger vessels were seen in all constructs, samples receiving delayed BMP treatment demonstrated significantly greater neovascularization. Delayed application of BMP was less effective for heterotopic bone formation than simultaneous application. A central channel allows homogeneous bone induction directly from the centre of the blocks.

AB - When bone morphogenetic protein (BMP) is delivered to matrices in vivo may affect tissue engineered bone constructs for jaw reconstruction after cancer surgery. This study compared the effects of BMP application at different times after matrix implantation for heterotopic bone induction in a rat model. Hydroxyapatite blocks were implanted unilaterally onto the surface of the latissimus dorsi muscle. A second block was implanted onto the contralateral muscle after 1, 2 or 4 weeks and 200 μg rhBMP-2 was injected into the blocks on both sides. Bone formation and density inside the blocks was analysed by CT and histology. 8 weeks after BMP application increases in bone density within the scaffolds were most pronounced in the simultaneous application group (179 HU). Less pronounced increases were observed for the 1 (65 HU), 2 (58 HU) and 4 (31 HU; p<0.0001) week delay group. Homogeneous bone induction started from the central channel of the blocks. Capillaries and larger vessels were seen in all constructs, samples receiving delayed BMP treatment demonstrated significantly greater neovascularization. Delayed application of BMP was less effective for heterotopic bone formation than simultaneous application. A central channel allows homogeneous bone induction directly from the centre of the blocks.

KW - Absorbable Implants

KW - Animals

KW - Bone Matrix

KW - Bone Morphogenetic Protein 2

KW - Bone Substitutes

KW - Drug Administration Schedule

KW - Drug Delivery Systems

KW - Female

KW - Hydroxyapatites

KW - Implants, Experimental

KW - Osseointegration

KW - Osteogenesis

KW - Rats

KW - Rats, Inbred Lew

KW - Time Factors

KW - Tissue Engineering

KW - Tissue Scaffolds

U2 - 10.1016/j.ijom.2012.03.031

DO - 10.1016/j.ijom.2012.03.031

M3 - SCORING: Journal article

C2 - 22652448

VL - 41

SP - 1153

EP - 1160

JO - INT J ORAL MAX SURG

JF - INT J ORAL MAX SURG

SN - 0901-5027

IS - 9

ER -