Enabling X-ray fluorescence imaging for in vivo immune cell tracking
Beteiligte Einrichtungen
Abstract
The infiltration of immune cells into sites of inflammation is one key feature of immune mediated inflammatory diseases. A detailed assessment of the in vivo dynamics of relevant cell subtypes could booster the understanding of this disease and the development of novel therapies. We show in detail how advanced X-ray fluorescence imaging enables such quantitative in vivo cell tracking, offering solutions that could pave the way beyond what other imaging modalities provide today. The key for this achievement is a detailed study of the spectral background contribution from multiple Compton scattering in a mouse-scaled object when this is scanned with a monochromatic pencil X-ray beam from a synchrotron. Under optimal conditions, the detection sensitivity is sufficient for detecting local accumulations of the labelled immune cells, hence providing experimental demonstration of in vivo immune cell tracking in mice.
Bibliografische Daten
Originalsprache | Englisch |
---|---|
ISSN | 2045-2322 |
DOIs | |
Status | Veröffentlicht - 17.07.2023 |
Anmerkungen des Dekanats
© 2023. The Author(s).
PubMed | 37460665 |
---|