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Electroporation-based gene transfer for efficient transfection of neural precursor cells

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Electroporation-based gene transfer for efficient transfection of neural precursor cells. / Richard, Ines; Ader, Marius; Sytnyk, Vladimir; Dityatev, Alexander; Richard, Gisbert; Schachner, Melitta; Bartsch, Udo.

in: MOL BRAIN RES, Jahrgang 138, Nr. 2, 18.08.2005, S. 182-90.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Richard, I, Ader, M, Sytnyk, V, Dityatev, A, Richard, G, Schachner, M & Bartsch, U 2005, 'Electroporation-based gene transfer for efficient transfection of neural precursor cells', MOL BRAIN RES, Jg. 138, Nr. 2, S. 182-90. https://doi.org/10.1016/j.molbrainres.2005.04.010

APA

Richard, I., Ader, M., Sytnyk, V., Dityatev, A., Richard, G., Schachner, M., & Bartsch, U. (2005). Electroporation-based gene transfer for efficient transfection of neural precursor cells. MOL BRAIN RES, 138(2), 182-90. https://doi.org/10.1016/j.molbrainres.2005.04.010

Vancouver

Richard I, Ader M, Sytnyk V, Dityatev A, Richard G, Schachner M et al. Electroporation-based gene transfer for efficient transfection of neural precursor cells. MOL BRAIN RES. 2005 Aug 18;138(2):182-90. https://doi.org/10.1016/j.molbrainres.2005.04.010

Bibtex

@article{77fad67cd5de4c70a54c05f33569825e,
title = "Electroporation-based gene transfer for efficient transfection of neural precursor cells",
abstract = "Transplantation of neural precursor cells (NPCs) is a potential tool to replace dysfunctional or degenerated neuronal or glial cell types in the central nervous system. Furthermore, transplantation of genetically engineered neural precursor cells might provide a strategy to target therapeutic gene products to the diseased nervous system. Here, we describe a novel and highly efficient electroporation-based transfection protocol for mitogen-expanded mouse NPCs. Transfection of NPCs with the reporter gene enhanced green fluorescent protein (EGFP) or the neural adhesion molecule L1 revealed transfection efficacies of more than 70% as estimated by the number of EGFP-positive or L1-immunoreactive cells 1 day after transfection in vitro. The percentage of EGFP- or L1-positive cells decreased with increasing time in culture. Positive cells were detectable for up to 3 weeks after transfection. When EGFP- or L1-transfected NPCs were grafted into the retina of adult wild-type or L1-deficient mice, they differentiated into glial cells some of which expressed EGFP and L1 for up to 2 and 3 weeks, respectively, the longest post-transplantation periods investigated.",
keywords = "Animals, Antigens, CD56, Cell Count, Cell Differentiation, Cells, Cultured, Electroporation, Genes, Reporter, Green Fluorescent Proteins, Mice, Mice, Inbred C57BL, Mice, Transgenic, Neuroglia, Neurons, Stem Cell Transplantation, Stem Cells, Transfection, Up-Regulation, Journal Article, Research Support, Non-U.S. Gov't",
author = "Ines Richard and Marius Ader and Vladimir Sytnyk and Alexander Dityatev and Gisbert Richard and Melitta Schachner and Udo Bartsch",
year = "2005",
month = aug,
day = "18",
doi = "10.1016/j.molbrainres.2005.04.010",
language = "English",
volume = "138",
pages = "182--90",
number = "2",

}

RIS

TY - JOUR

T1 - Electroporation-based gene transfer for efficient transfection of neural precursor cells

AU - Richard, Ines

AU - Ader, Marius

AU - Sytnyk, Vladimir

AU - Dityatev, Alexander

AU - Richard, Gisbert

AU - Schachner, Melitta

AU - Bartsch, Udo

PY - 2005/8/18

Y1 - 2005/8/18

N2 - Transplantation of neural precursor cells (NPCs) is a potential tool to replace dysfunctional or degenerated neuronal or glial cell types in the central nervous system. Furthermore, transplantation of genetically engineered neural precursor cells might provide a strategy to target therapeutic gene products to the diseased nervous system. Here, we describe a novel and highly efficient electroporation-based transfection protocol for mitogen-expanded mouse NPCs. Transfection of NPCs with the reporter gene enhanced green fluorescent protein (EGFP) or the neural adhesion molecule L1 revealed transfection efficacies of more than 70% as estimated by the number of EGFP-positive or L1-immunoreactive cells 1 day after transfection in vitro. The percentage of EGFP- or L1-positive cells decreased with increasing time in culture. Positive cells were detectable for up to 3 weeks after transfection. When EGFP- or L1-transfected NPCs were grafted into the retina of adult wild-type or L1-deficient mice, they differentiated into glial cells some of which expressed EGFP and L1 for up to 2 and 3 weeks, respectively, the longest post-transplantation periods investigated.

AB - Transplantation of neural precursor cells (NPCs) is a potential tool to replace dysfunctional or degenerated neuronal or glial cell types in the central nervous system. Furthermore, transplantation of genetically engineered neural precursor cells might provide a strategy to target therapeutic gene products to the diseased nervous system. Here, we describe a novel and highly efficient electroporation-based transfection protocol for mitogen-expanded mouse NPCs. Transfection of NPCs with the reporter gene enhanced green fluorescent protein (EGFP) or the neural adhesion molecule L1 revealed transfection efficacies of more than 70% as estimated by the number of EGFP-positive or L1-immunoreactive cells 1 day after transfection in vitro. The percentage of EGFP- or L1-positive cells decreased with increasing time in culture. Positive cells were detectable for up to 3 weeks after transfection. When EGFP- or L1-transfected NPCs were grafted into the retina of adult wild-type or L1-deficient mice, they differentiated into glial cells some of which expressed EGFP and L1 for up to 2 and 3 weeks, respectively, the longest post-transplantation periods investigated.

KW - Animals

KW - Antigens, CD56

KW - Cell Count

KW - Cell Differentiation

KW - Cells, Cultured

KW - Electroporation

KW - Genes, Reporter

KW - Green Fluorescent Proteins

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Transgenic

KW - Neuroglia

KW - Neurons

KW - Stem Cell Transplantation

KW - Stem Cells

KW - Transfection

KW - Up-Regulation

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.molbrainres.2005.04.010

DO - 10.1016/j.molbrainres.2005.04.010

M3 - SCORING: Journal article

C2 - 15908040

VL - 138

SP - 182

EP - 190

IS - 2

ER -