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Efficient optogenetic silencing of neurotransmitter release with a mosquito rhodopsin

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Efficient optogenetic silencing of neurotransmitter release with a mosquito rhodopsin. / Mahn, Mathias; Saraf-Sinik, Inbar; Patil, Pritish; Pulin, Mauro; Bitton, Eyal; Karalis, Nikolaos; Bruentgens, Felicitas; Palgi, Shaked; Gat, Asaf; Dine, Julien; Wietek, Jonas; Davidi, Ido; Levy, Rivka; Litvin, Anna; Zhou, Fangmin; Sauter, Kathrin; Soba, Peter; Schmitz, Dietmar; Lüthi, Andreas; Rost, Benjamin R; Wiegert, J Simon; Yizhar, Ofer.

in: NEURON, Jahrgang 109, Nr. 10, 19.05.2021, S. 1621-1635.e8.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Mahn, M, Saraf-Sinik, I, Patil, P, Pulin, M, Bitton, E, Karalis, N, Bruentgens, F, Palgi, S, Gat, A, Dine, J, Wietek, J, Davidi, I, Levy, R, Litvin, A, Zhou, F, Sauter, K, Soba, P, Schmitz, D, Lüthi, A, Rost, BR, Wiegert, JS & Yizhar, O 2021, 'Efficient optogenetic silencing of neurotransmitter release with a mosquito rhodopsin', NEURON, Jg. 109, Nr. 10, S. 1621-1635.e8. https://doi.org/10.1016/j.neuron.2021.03.013

APA

Mahn, M., Saraf-Sinik, I., Patil, P., Pulin, M., Bitton, E., Karalis, N., Bruentgens, F., Palgi, S., Gat, A., Dine, J., Wietek, J., Davidi, I., Levy, R., Litvin, A., Zhou, F., Sauter, K., Soba, P., Schmitz, D., Lüthi, A., ... Yizhar, O. (2021). Efficient optogenetic silencing of neurotransmitter release with a mosquito rhodopsin. NEURON, 109(10), 1621-1635.e8. https://doi.org/10.1016/j.neuron.2021.03.013

Vancouver

Mahn M, Saraf-Sinik I, Patil P, Pulin M, Bitton E, Karalis N et al. Efficient optogenetic silencing of neurotransmitter release with a mosquito rhodopsin. NEURON. 2021 Mai 19;109(10):1621-1635.e8. https://doi.org/10.1016/j.neuron.2021.03.013

Bibtex

@article{8e11c39ed8fb492698a47fbadc7eb80e,
title = "Efficient optogenetic silencing of neurotransmitter release with a mosquito rhodopsin",
abstract = "Information is carried between brain regions through neurotransmitter release from axonal presynaptic terminals. Understanding the functional roles of defined neuronal projection pathways requires temporally precise manipulation of their activity. However, existing inhibitory optogenetic tools have low efficacy and off-target effects when applied to presynaptic terminals, while chemogenetic tools are difficult to control in space and time. Here, we show that a targeting-enhanced mosquito homolog of the vertebrate encephalopsin (eOPN3) can effectively suppress synaptic transmission through the Gi/o signaling pathway. Brief illumination of presynaptic terminals expressing eOPN3 triggers a lasting suppression of synaptic output that recovers spontaneously within minutes in vitro and in vivo. In freely moving mice, eOPN3-mediated suppression of dopaminergic nigrostriatal afferents induces a reversible ipsiversive rotational bias. We conclude that eOPN3 can be used to selectively suppress neurotransmitter release at presynaptic terminals with high spatiotemporal precision, opening new avenues for functional interrogation of long-range neuronal circuits in vivo.",
author = "Mathias Mahn and Inbar Saraf-Sinik and Pritish Patil and Mauro Pulin and Eyal Bitton and Nikolaos Karalis and Felicitas Bruentgens and Shaked Palgi and Asaf Gat and Julien Dine and Jonas Wietek and Ido Davidi and Rivka Levy and Anna Litvin and Fangmin Zhou and Kathrin Sauter and Peter Soba and Dietmar Schmitz and Andreas L{\"u}thi and Rost, {Benjamin R} and Wiegert, {J Simon} and Ofer Yizhar",
note = "Copyright {\textcopyright} 2021 Elsevier Inc. All rights reserved.",
year = "2021",
month = may,
day = "19",
doi = "10.1016/j.neuron.2021.03.013",
language = "English",
volume = "109",
pages = "1621--1635.e8",
journal = "NEURON",
issn = "0896-6273",
publisher = "Cell Press",
number = "10",

}

RIS

TY - JOUR

T1 - Efficient optogenetic silencing of neurotransmitter release with a mosquito rhodopsin

AU - Mahn, Mathias

AU - Saraf-Sinik, Inbar

AU - Patil, Pritish

AU - Pulin, Mauro

AU - Bitton, Eyal

AU - Karalis, Nikolaos

AU - Bruentgens, Felicitas

AU - Palgi, Shaked

AU - Gat, Asaf

AU - Dine, Julien

AU - Wietek, Jonas

AU - Davidi, Ido

AU - Levy, Rivka

AU - Litvin, Anna

AU - Zhou, Fangmin

AU - Sauter, Kathrin

AU - Soba, Peter

AU - Schmitz, Dietmar

AU - Lüthi, Andreas

AU - Rost, Benjamin R

AU - Wiegert, J Simon

AU - Yizhar, Ofer

N1 - Copyright © 2021 Elsevier Inc. All rights reserved.

PY - 2021/5/19

Y1 - 2021/5/19

N2 - Information is carried between brain regions through neurotransmitter release from axonal presynaptic terminals. Understanding the functional roles of defined neuronal projection pathways requires temporally precise manipulation of their activity. However, existing inhibitory optogenetic tools have low efficacy and off-target effects when applied to presynaptic terminals, while chemogenetic tools are difficult to control in space and time. Here, we show that a targeting-enhanced mosquito homolog of the vertebrate encephalopsin (eOPN3) can effectively suppress synaptic transmission through the Gi/o signaling pathway. Brief illumination of presynaptic terminals expressing eOPN3 triggers a lasting suppression of synaptic output that recovers spontaneously within minutes in vitro and in vivo. In freely moving mice, eOPN3-mediated suppression of dopaminergic nigrostriatal afferents induces a reversible ipsiversive rotational bias. We conclude that eOPN3 can be used to selectively suppress neurotransmitter release at presynaptic terminals with high spatiotemporal precision, opening new avenues for functional interrogation of long-range neuronal circuits in vivo.

AB - Information is carried between brain regions through neurotransmitter release from axonal presynaptic terminals. Understanding the functional roles of defined neuronal projection pathways requires temporally precise manipulation of their activity. However, existing inhibitory optogenetic tools have low efficacy and off-target effects when applied to presynaptic terminals, while chemogenetic tools are difficult to control in space and time. Here, we show that a targeting-enhanced mosquito homolog of the vertebrate encephalopsin (eOPN3) can effectively suppress synaptic transmission through the Gi/o signaling pathway. Brief illumination of presynaptic terminals expressing eOPN3 triggers a lasting suppression of synaptic output that recovers spontaneously within minutes in vitro and in vivo. In freely moving mice, eOPN3-mediated suppression of dopaminergic nigrostriatal afferents induces a reversible ipsiversive rotational bias. We conclude that eOPN3 can be used to selectively suppress neurotransmitter release at presynaptic terminals with high spatiotemporal precision, opening new avenues for functional interrogation of long-range neuronal circuits in vivo.

U2 - 10.1016/j.neuron.2021.03.013

DO - 10.1016/j.neuron.2021.03.013

M3 - SCORING: Journal article

C2 - 33979634

VL - 109

SP - 1621-1635.e8

JO - NEURON

JF - NEURON

SN - 0896-6273

IS - 10

ER -