Efficient in vitro generation of adult multipotent cells from mobilized peripheral blood CD133+ cells
Standard
Efficient in vitro generation of adult multipotent cells from mobilized peripheral blood CD133+ cells. / Kuçi, S; Kuçi, Z; Schmid, S; Seitz, G; Müller, I; Dufke, A; Leimig, T; Murti, G; Jurecic, R; Schumm, M; Lang, P; Bruchelt, G; Bader, P; Klingebiel, T; Niethammer, D; Handgretinger, R.
in: CELL PROLIFERAT, Jahrgang 41, Nr. 1, 02.2008, S. 12-27.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Efficient in vitro generation of adult multipotent cells from mobilized peripheral blood CD133+ cells
AU - Kuçi, S
AU - Kuçi, Z
AU - Schmid, S
AU - Seitz, G
AU - Müller, I
AU - Dufke, A
AU - Leimig, T
AU - Murti, G
AU - Jurecic, R
AU - Schumm, M
AU - Lang, P
AU - Bruchelt, G
AU - Bader, P
AU - Klingebiel, T
AU - Niethammer, D
AU - Handgretinger, R
PY - 2008/2
Y1 - 2008/2
N2 - OBJECTIVES: To generate non-haematopoietic tissues from mobilized haematopoietic CD133(+) stem cells.MATERIALS AND METHODS: Mobilized peripheral blood CD133(+) cells from adult healthy donors were used. In vitro ability of highly enriched CD133(+) cells from mobilized peripheral blood to generate multipotent cells, and their potential to give rise to cells with characteristics of neuroectoderm, endoderm and mesoderm layers was investigated.RESULTS: We found that a recently identified population of CD45(+) adherent cells generated in vitro after culture of highly purified CD133(+) cells for 3-5 weeks with Flt3/Flk2 ligand and interleukin-6 can, in presence of the appropriate microenvironmental cues, differentiate into neural progenitor-like cells (NPLCs), hepatocyte-like cells and skeletal muscle-like cells. We have termed them to be adult multipotent haematopoietic cells (AMHCs). AMHC-derived NPLCs expressed morphological, phenotypic and molecular markers associated with primary neural progenitor cells. They can differentiate into astrocyte-like cells, neuronal-like cells and oligodendrocyte-like cells. Moreover, AMHC-derived NPLCs produced 3,4-dihydrophenylalanine and dopamine and expressed voltage-activated ion channels, suggesting their functional maturation. In addition, AMHC-derived hepatocyte-like cells and skeletal muscle-like cells, showed typical morphological features and expressed primary tissue-associated proteins.CONCLUSION: Our data demonstrate that AMHCs may therefore serve as a novel source of adult multipotent cells for autologous replacement cell therapies.
AB - OBJECTIVES: To generate non-haematopoietic tissues from mobilized haematopoietic CD133(+) stem cells.MATERIALS AND METHODS: Mobilized peripheral blood CD133(+) cells from adult healthy donors were used. In vitro ability of highly enriched CD133(+) cells from mobilized peripheral blood to generate multipotent cells, and their potential to give rise to cells with characteristics of neuroectoderm, endoderm and mesoderm layers was investigated.RESULTS: We found that a recently identified population of CD45(+) adherent cells generated in vitro after culture of highly purified CD133(+) cells for 3-5 weeks with Flt3/Flk2 ligand and interleukin-6 can, in presence of the appropriate microenvironmental cues, differentiate into neural progenitor-like cells (NPLCs), hepatocyte-like cells and skeletal muscle-like cells. We have termed them to be adult multipotent haematopoietic cells (AMHCs). AMHC-derived NPLCs expressed morphological, phenotypic and molecular markers associated with primary neural progenitor cells. They can differentiate into astrocyte-like cells, neuronal-like cells and oligodendrocyte-like cells. Moreover, AMHC-derived NPLCs produced 3,4-dihydrophenylalanine and dopamine and expressed voltage-activated ion channels, suggesting their functional maturation. In addition, AMHC-derived hepatocyte-like cells and skeletal muscle-like cells, showed typical morphological features and expressed primary tissue-associated proteins.CONCLUSION: Our data demonstrate that AMHCs may therefore serve as a novel source of adult multipotent cells for autologous replacement cell therapies.
KW - Adult
KW - Antigens, CD
KW - Base Sequence
KW - Cell Differentiation
KW - Chromatography, High Pressure Liquid
KW - DNA Primers
KW - Dihydroxyphenylalanine
KW - Dopamine
KW - Glycoproteins
KW - Humans
KW - In Vitro Techniques
KW - Multipotent Stem Cells
KW - Patch-Clamp Techniques
KW - Peptides
KW - Reverse Transcriptase Polymerase Chain Reaction
U2 - 10.1111/j.1365-2184.2007.00502.x
DO - 10.1111/j.1365-2184.2007.00502.x
M3 - SCORING: Journal article
C2 - 18211283
VL - 41
SP - 12
EP - 27
JO - CELL PROLIFERAT
JF - CELL PROLIFERAT
SN - 0960-7722
IS - 1
ER -