Effects of Vitamin B Complex and Vitamin C on Human Skin Cells

TY - JOUR

T1 - Effects of Vitamin B Complex and Vitamin C on Human Skin Cells

T2 - Is the Perceived Effect Measurable?

AU - Rembe, Julian-Dario

AU - Fromm-Dornieden, Carolin

AU - Stuermer, Ewa Klara

PY - 2018/5

Y1 - 2018/5

N2 - OBJECTIVE: Vitamins are essential for human health. In terms of local application for wound healing, vitamins' positive effect remains unclear. However, because of the regular appearance of nutritional deficiency in chronic wound patients, a favorable impact of locally applied vitamins can be hypothesized.METHODS: Vitamins B3, B5, B6, B7, B9, B10, B12, and C individually as well as different combinations of B vitamins were investigated regarding their ability to promote proliferation and migration of human skin fibroblasts and keratinocytes. Proliferation assays with and without bacterial challenge, immunocytochemical staining, and scratch assay were used to determine the most effective combination(s).MAIN RESULTS: Some vitamin combinations showed a positive impact on proliferation, especially for keratinocytes after 72 hours. In terms of wound closure, the combinations B9 and B12; B3, B5, B6, and B10; and B3, B5, and B7 improved closure rates by 25% to 30%. The improved closure rates are also reflected by immunocytochemically detected upregulation of the migration marker CXCR4 for several combinations.CONCLUSIONS: Certain combinations of B vitamins demonstrate a positive influence on human keratinocytes and fibroblasts. Vitamins especially promoted fibroblast migration, and a statistically significant induction of keratinocyte proliferation was observed. Therefore, local vitamin application could benefit the physiologic wound healing process.

AB - OBJECTIVE: Vitamins are essential for human health. In terms of local application for wound healing, vitamins' positive effect remains unclear. However, because of the regular appearance of nutritional deficiency in chronic wound patients, a favorable impact of locally applied vitamins can be hypothesized.METHODS: Vitamins B3, B5, B6, B7, B9, B10, B12, and C individually as well as different combinations of B vitamins were investigated regarding their ability to promote proliferation and migration of human skin fibroblasts and keratinocytes. Proliferation assays with and without bacterial challenge, immunocytochemical staining, and scratch assay were used to determine the most effective combination(s).MAIN RESULTS: Some vitamin combinations showed a positive impact on proliferation, especially for keratinocytes after 72 hours. In terms of wound closure, the combinations B9 and B12; B3, B5, B6, and B10; and B3, B5, and B7 improved closure rates by 25% to 30%. The improved closure rates are also reflected by immunocytochemically detected upregulation of the migration marker CXCR4 for several combinations.CONCLUSIONS: Certain combinations of B vitamins demonstrate a positive influence on human keratinocytes and fibroblasts. Vitamins especially promoted fibroblast migration, and a statistically significant induction of keratinocyte proliferation was observed. Therefore, local vitamin application could benefit the physiologic wound healing process.

KW - Ascorbic Acid/pharmacology

KW - Cell Movement/drug effects

KW - Cell Proliferation/drug effects

KW - Cells, Cultured

KW - Female

KW - Fibroblasts/drug effects

KW - Germany

KW - Humans

KW - In Vitro Techniques

KW - Keratinocytes/drug effects

KW - Male

KW - Sensitivity and Specificity

KW - Skin/cytology

KW - Vitamin B Complex/pharmacology

KW - Wound Healing/drug effects

U2 - 10.1097/01.ASW.0000531351.85866.d9

DO - 10.1097/01.ASW.0000531351.85866.d9

M3 - SCORING: Journal article

C2 - 29672394

VL - 31

SP - 225

EP - 233

JO - ADV SKIN WOUND CARE

JF - ADV SKIN WOUND CARE

SN - 1527-7941

IS - 5

ER -