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Effect of dexamethasone on the lymphocytic Na+/H+ antiporter activity

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Effect of dexamethasone on the lymphocytic Na+/H+ antiporter activity. / Tepel, M; Pytlik, M; van der Giet, M; Schlüter, H; Jankowski, J; Zidek, W.

in: J HYPERTENS, Jahrgang 17, Nr. 11, 11.1999, S. 1553-6.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Tepel, M, Pytlik, M, van der Giet, M, Schlüter, H, Jankowski, J & Zidek, W 1999, 'Effect of dexamethasone on the lymphocytic Na+/H+ antiporter activity', J HYPERTENS, Jg. 17, Nr. 11, S. 1553-6.

APA

Tepel, M., Pytlik, M., van der Giet, M., Schlüter, H., Jankowski, J., & Zidek, W. (1999). Effect of dexamethasone on the lymphocytic Na+/H+ antiporter activity. J HYPERTENS, 17(11), 1553-6.

Vancouver

Tepel M, Pytlik M, van der Giet M, Schlüter H, Jankowski J, Zidek W. Effect of dexamethasone on the lymphocytic Na+/H+ antiporter activity. J HYPERTENS. 1999 Nov;17(11):1553-6.

Bibtex

@article{98be4cb87d9944e18cd90177f96a2feb,
title = "Effect of dexamethasone on the lymphocytic Na+/H+ antiporter activity",
abstract = "OBJECTIVE: The effects of short-term administration of dexamethasone on sodium/proton antiporter (Na+/H+ antiporter) in human lymphocytes were investigated in vitro.METHODS: Cytosolic pHi in lymphocytes was measured spectrophotometrically using the pH-sensitive fluorescent dye 2'-7'-bis-carboxyethyl-5(6)-carboxyfluorescein at 530 nm with excitation wavelengths of 440 and 530 nm. The Na+/H+ antiporter activity was determined after intracellular acidification using 100 mmol/l propionic acid.RESULTS: The addition of 1 micromol/l dexamethasone significantly reduced cytosolic pHi from 7.44+/-0.03 to 7.25+/-0.05 (mean +/- SEM; P<0.01). Incubation with dexamethasone for 40 min significantly reduced the Na+/ H+ antiporter activity from (9.19+/-0.61)x10(-3) pHi/s (n = 22) to (7.23+/-0.49)x10(-3) pHi/s (n = 22; P<0.01). The effect of dexamethasone was time and concentration dependent The apparent affinity of the Na+/H+ antiporter was not significantly different in the absence or presence of dexamethasone. The inhibition of the Na+/H+ antiporter by dexamethasone was abolished in the presence of the glucocorticoid receptor blocker, mifepristone.CONCLUSION: Dexamethasone directly inhibits the Na+/H+ antiporter using a receptor-dependent pathway. This effect may be important for pharmacological side effects such as glucocorticoid-induced hypertension.",
keywords = "Cytosol, Dexamethasone, Glucocorticoids, Hormone Antagonists, Humans, Hydrogen-Ion Concentration, Lymphocytes, Mifepristone, Osmolar Concentration, Sodium-Hydrogen Exchangers, Time Factors, Journal Article",
author = "M Tepel and M Pytlik and {van der Giet}, M and H Schl{\"u}ter and J Jankowski and W Zidek",
year = "1999",
month = nov,
language = "English",
volume = "17",
pages = "1553--6",
journal = "J HYPERTENS",
issn = "0263-6352",
publisher = "Lippincott Williams and Wilkins",
number = "11",

}

RIS

TY - JOUR

T1 - Effect of dexamethasone on the lymphocytic Na+/H+ antiporter activity

AU - Tepel, M

AU - Pytlik, M

AU - van der Giet, M

AU - Schlüter, H

AU - Jankowski, J

AU - Zidek, W

PY - 1999/11

Y1 - 1999/11

N2 - OBJECTIVE: The effects of short-term administration of dexamethasone on sodium/proton antiporter (Na+/H+ antiporter) in human lymphocytes were investigated in vitro.METHODS: Cytosolic pHi in lymphocytes was measured spectrophotometrically using the pH-sensitive fluorescent dye 2'-7'-bis-carboxyethyl-5(6)-carboxyfluorescein at 530 nm with excitation wavelengths of 440 and 530 nm. The Na+/H+ antiporter activity was determined after intracellular acidification using 100 mmol/l propionic acid.RESULTS: The addition of 1 micromol/l dexamethasone significantly reduced cytosolic pHi from 7.44+/-0.03 to 7.25+/-0.05 (mean +/- SEM; P<0.01). Incubation with dexamethasone for 40 min significantly reduced the Na+/ H+ antiporter activity from (9.19+/-0.61)x10(-3) pHi/s (n = 22) to (7.23+/-0.49)x10(-3) pHi/s (n = 22; P<0.01). The effect of dexamethasone was time and concentration dependent The apparent affinity of the Na+/H+ antiporter was not significantly different in the absence or presence of dexamethasone. The inhibition of the Na+/H+ antiporter by dexamethasone was abolished in the presence of the glucocorticoid receptor blocker, mifepristone.CONCLUSION: Dexamethasone directly inhibits the Na+/H+ antiporter using a receptor-dependent pathway. This effect may be important for pharmacological side effects such as glucocorticoid-induced hypertension.

AB - OBJECTIVE: The effects of short-term administration of dexamethasone on sodium/proton antiporter (Na+/H+ antiporter) in human lymphocytes were investigated in vitro.METHODS: Cytosolic pHi in lymphocytes was measured spectrophotometrically using the pH-sensitive fluorescent dye 2'-7'-bis-carboxyethyl-5(6)-carboxyfluorescein at 530 nm with excitation wavelengths of 440 and 530 nm. The Na+/H+ antiporter activity was determined after intracellular acidification using 100 mmol/l propionic acid.RESULTS: The addition of 1 micromol/l dexamethasone significantly reduced cytosolic pHi from 7.44+/-0.03 to 7.25+/-0.05 (mean +/- SEM; P<0.01). Incubation with dexamethasone for 40 min significantly reduced the Na+/ H+ antiporter activity from (9.19+/-0.61)x10(-3) pHi/s (n = 22) to (7.23+/-0.49)x10(-3) pHi/s (n = 22; P<0.01). The effect of dexamethasone was time and concentration dependent The apparent affinity of the Na+/H+ antiporter was not significantly different in the absence or presence of dexamethasone. The inhibition of the Na+/H+ antiporter by dexamethasone was abolished in the presence of the glucocorticoid receptor blocker, mifepristone.CONCLUSION: Dexamethasone directly inhibits the Na+/H+ antiporter using a receptor-dependent pathway. This effect may be important for pharmacological side effects such as glucocorticoid-induced hypertension.

KW - Cytosol

KW - Dexamethasone

KW - Glucocorticoids

KW - Hormone Antagonists

KW - Humans

KW - Hydrogen-Ion Concentration

KW - Lymphocytes

KW - Mifepristone

KW - Osmolar Concentration

KW - Sodium-Hydrogen Exchangers

KW - Time Factors

KW - Journal Article

M3 - SCORING: Journal article

C2 - 10608467

VL - 17

SP - 1553

EP - 1556

JO - J HYPERTENS

JF - J HYPERTENS

SN - 0263-6352

IS - 11

ER -