Effect of chronic Giardia lamblia infection on epithelial transport and barrier function in human duodenum.
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Effect of chronic Giardia lamblia infection on epithelial transport and barrier function in human duodenum. / Troeger, Hanno; Epple, Hans-Joerg; Schneider, Thomas; Wahnschaffe, Ulrich; Ullrich, Reiner; Burchard, Gerd-Dieter; Jelinek, Tomas; Zeitz, Martin; Fromm, Michael; Schulzke, Joerg-Dieter.
in: GUT, Jahrgang 56, Nr. 3, 3, 2007, S. 328-335.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Effect of chronic Giardia lamblia infection on epithelial transport and barrier function in human duodenum.
AU - Troeger, Hanno
AU - Epple, Hans-Joerg
AU - Schneider, Thomas
AU - Wahnschaffe, Ulrich
AU - Ullrich, Reiner
AU - Burchard, Gerd-Dieter
AU - Jelinek, Tomas
AU - Zeitz, Martin
AU - Fromm, Michael
AU - Schulzke, Joerg-Dieter
PY - 2007
Y1 - 2007
N2 - BACKGROUND: Giardia lamblia causes infection of the small intestine, which leads to malabsorption and chronic diarrhoea. AIM: To characterise the inherent pathomechanisms of G lamblia infection. METHODS: Duodenal biopsy specimens from 13 patients with chronic giardiasis and from controls were obtained endoscopically. Short-circuit current (I(SC)) and mannitol fluxes were measured in miniaturised Ussing chambers. Epithelial and subepithelial resistances were determined by impedance spectroscopy. Mucosal morphometry was performed and tight junction proteins were characterised by immunoblotting. Apoptotic ratio was determined by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labelling staining. RESULTS: In giardiasis, mucosal surface area per unit serosa area was decreased to 75% (3%) of control, as a result of which epithelial resistance should increase. Instead, epithelial resistance of giardiasis biopsy specimens was decreased (19 (2) vs 25 (2) Omega cm(2); p
AB - BACKGROUND: Giardia lamblia causes infection of the small intestine, which leads to malabsorption and chronic diarrhoea. AIM: To characterise the inherent pathomechanisms of G lamblia infection. METHODS: Duodenal biopsy specimens from 13 patients with chronic giardiasis and from controls were obtained endoscopically. Short-circuit current (I(SC)) and mannitol fluxes were measured in miniaturised Ussing chambers. Epithelial and subepithelial resistances were determined by impedance spectroscopy. Mucosal morphometry was performed and tight junction proteins were characterised by immunoblotting. Apoptotic ratio was determined by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labelling staining. RESULTS: In giardiasis, mucosal surface area per unit serosa area was decreased to 75% (3%) of control, as a result of which epithelial resistance should increase. Instead, epithelial resistance of giardiasis biopsy specimens was decreased (19 (2) vs 25 (2) Omega cm(2); p
M3 - SCORING: Zeitschriftenaufsatz
VL - 56
SP - 328
EP - 335
JO - GUT
JF - GUT
SN - 0017-5749
IS - 3
M1 - 3
ER -