Effect of aqueous ozone on the NF-kappaB system
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Effect of aqueous ozone on the NF-kappaB system. / Huth, K C; Saugel, B; Jakob, F M; Cappello, C; Quirling, M; Paschos, E; Ern, K; Hickel, R; Brand, K.
in: J DENT RES, Jahrgang 86, Nr. 5, 01.05.2007, S. 451-6.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Effect of aqueous ozone on the NF-kappaB system
AU - Huth, K C
AU - Saugel, B
AU - Jakob, F M
AU - Cappello, C
AU - Quirling, M
AU - Paschos, E
AU - Ern, K
AU - Hickel, R
AU - Brand, K
PY - 2007/5/1
Y1 - 2007/5/1
N2 - Ozone has been proposed as an alternative oral antiseptic in dentistry, due to its antimicrobial power reported for gaseous and aqueous forms, the latter showing a high biocompatibility with mammalian cells. New therapeutic strategies for the treatment of periodontal disease and apical periodontitis should consider not only antibacterial effects, but also their influence on the host immune response. Therefore, our aim was to investigate the effect of aqueous ozone on the NF-kappaB system, a paradigm for inflammation-associated signaling/transcription. We showed that NF-kappaB activity in oral cells stimulated with TNF, and in periodontal ligament tissue from root surfaces of periodontally damaged teeth, was inhibited following incubation with ozonized medium. Under this treatment, IkappaBalpha proteolysis, cytokine expression, and kappaB-dependent transcription were prevented. Specific ozonized amino acids were shown to represent major inhibitory components of ozonized medium. In summary, our study establishes a condition under which aqueous ozone exerts inhibitory effects on the NF-kappaB system, suggesting that it has an anti-inflammatory capacity.
AB - Ozone has been proposed as an alternative oral antiseptic in dentistry, due to its antimicrobial power reported for gaseous and aqueous forms, the latter showing a high biocompatibility with mammalian cells. New therapeutic strategies for the treatment of periodontal disease and apical periodontitis should consider not only antibacterial effects, but also their influence on the host immune response. Therefore, our aim was to investigate the effect of aqueous ozone on the NF-kappaB system, a paradigm for inflammation-associated signaling/transcription. We showed that NF-kappaB activity in oral cells stimulated with TNF, and in periodontal ligament tissue from root surfaces of periodontally damaged teeth, was inhibited following incubation with ozonized medium. Under this treatment, IkappaBalpha proteolysis, cytokine expression, and kappaB-dependent transcription were prevented. Specific ozonized amino acids were shown to represent major inhibitory components of ozonized medium. In summary, our study establishes a condition under which aqueous ozone exerts inhibitory effects on the NF-kappaB system, suggesting that it has an anti-inflammatory capacity.
KW - Amino Acids
KW - Cells, Cultured
KW - Culture Media, Conditioned
KW - Cytokines
KW - Epithelial Cells
KW - Fibroblasts
KW - HeLa Cells
KW - Humans
KW - I-kappa B Proteins
KW - NF-kappa B
KW - Oxidants, Photochemical
KW - Ozone
KW - Periodontal Ligament
KW - Periodontitis
KW - Signal Transduction
KW - Transcriptional Activation
KW - Tumor Necrosis Factor-alpha
M3 - SCORING: Journal article
C2 - 17452567
VL - 86
SP - 451
EP - 456
JO - J DENT RES
JF - J DENT RES
SN - 0022-0345
IS - 5
ER -