During apoptosis HMGB1 is translocated into apoptotic cell-derived membranous vesicles
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During apoptosis HMGB1 is translocated into apoptotic cell-derived membranous vesicles. / Schiller, Martin; Heyder, Petra; Ziegler, Saskia; Nießen, Anna; Claßen, Laura; Lauffer, Anna; Lorenz, Hanns-Martin.
in: AUTOIMMUNITY, Jahrgang 46, Nr. 5, 08.2013, S. 342-6.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - During apoptosis HMGB1 is translocated into apoptotic cell-derived membranous vesicles
AU - Schiller, Martin
AU - Heyder, Petra
AU - Ziegler, Saskia
AU - Nießen, Anna
AU - Claßen, Laura
AU - Lauffer, Anna
AU - Lorenz, Hanns-Martin
PY - 2013/8
Y1 - 2013/8
N2 - High mobility group box protein B1 (HMGB1), a nuclear protein reportedly involved in the structural organisation of DNA, is released from necrotic cells or upon cellular activation. After its release into the extracellular space, HMGB1 serves as a mediator of inflammation. In contrast to necrotic cells, apoptotic ones usually do not release HMGB1. Formation and release of membranous vesicles is a well-known feature of apoptotic cell death. Only recently, subcellular membrane vesicles, such as those released during apoptotic cell death have been identified as immune regulators and as mediators of cell to cell communication. We and others have previously detected nuclear antigens within apoptosis-released membranous vesicles and HMGB1 together with nuclear antigens has been discussed to be a key player in etiology and pathogenesis of autoimmune diseases. On this background, we analysed whether HMGB1 is included in the membranous vesicles generated by apoptosing cells. Employing immune blots we observed abundand amounts of HMGB1 in the fraction of the small membraneous particles isolated from cell culture supernatants and conclude that HMGB1 is translocated into vesicles generated during apoptosis.
AB - High mobility group box protein B1 (HMGB1), a nuclear protein reportedly involved in the structural organisation of DNA, is released from necrotic cells or upon cellular activation. After its release into the extracellular space, HMGB1 serves as a mediator of inflammation. In contrast to necrotic cells, apoptotic ones usually do not release HMGB1. Formation and release of membranous vesicles is a well-known feature of apoptotic cell death. Only recently, subcellular membrane vesicles, such as those released during apoptotic cell death have been identified as immune regulators and as mediators of cell to cell communication. We and others have previously detected nuclear antigens within apoptosis-released membranous vesicles and HMGB1 together with nuclear antigens has been discussed to be a key player in etiology and pathogenesis of autoimmune diseases. On this background, we analysed whether HMGB1 is included in the membranous vesicles generated by apoptosing cells. Employing immune blots we observed abundand amounts of HMGB1 in the fraction of the small membraneous particles isolated from cell culture supernatants and conclude that HMGB1 is translocated into vesicles generated during apoptosis.
KW - Apoptosis/immunology
KW - Cells, Cultured
KW - HMGB1 Protein/metabolism
KW - Humans
KW - Inflammation Mediators/metabolism
KW - Lymphocyte Activation/immunology
KW - Protein Transport/immunology
KW - R-SNARE Proteins/metabolism
U2 - 10.3109/08916934.2012.750302
DO - 10.3109/08916934.2012.750302
M3 - SCORING: Journal article
C2 - 23194089
VL - 46
SP - 342
EP - 346
JO - AUTOIMMUNITY
JF - AUTOIMMUNITY
SN - 0891-6934
IS - 5
ER -