Doping of casted silk fibroin membranes with extracellular vesicles for regenerative therapy: a proof of concept
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Doping of casted silk fibroin membranes with extracellular vesicles for regenerative therapy: a proof of concept. / Fuest, Sandra; Salviano-Silva, Amanda; Maire, Cecile L; Xu, Yong; Apel, Christian; Grust, Audrey Laure Céline; Delle Coste, Arianna; Gosau, Martin; Ricklefs, Franz L; Smeets, Ralf.
in: SCI REP-UK, Jahrgang 14, Nr. 1, 12.02.2024, S. 3553.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung
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TY - JOUR
T1 - Doping of casted silk fibroin membranes with extracellular vesicles for regenerative therapy: a proof of concept
AU - Fuest, Sandra
AU - Salviano-Silva, Amanda
AU - Maire, Cecile L
AU - Xu, Yong
AU - Apel, Christian
AU - Grust, Audrey Laure Céline
AU - Delle Coste, Arianna
AU - Gosau, Martin
AU - Ricklefs, Franz L
AU - Smeets, Ralf
N1 - © 2024. The Author(s).
PY - 2024/2/12
Y1 - 2024/2/12
N2 - Bioactive material concepts for targeted therapy have been an important research focus in regenerative medicine for years. The aim of this study was to investigate a proof-of-concept composite structure in the form of a membrane made of natural silk fibroin (SF) and extracellular vesicles (EVs) from gingival fibroblasts. EVs have multiple abilities to act on their target cell and can thus play crucial roles in both physiology and regeneration. This study used pH neutral, degradable SF-based membranes, which have excellent cell- and tissue-specific properties, as the carrier material. The characterization of the vesicles showed a size range between 120 and 180 nm and a high expression of the usual EV markers (e.g. CD9, CD63 and CD81), measured by nanoparticle tracking analysis (NTA) and single-EV flow analysis (IFCM). An initial integration of the EVs into the membrane was analyzed using scanning and transmission electron microscopy (SEM and TEM) and vesicles were successfully detected, even if they were not homogeneously distributed in the membrane. Using direct and indirect tests, the cytocompatibility of the membranes with and without EVs could be proven and showed significant differences compared to the toxic control (p < 0.05). Additionally, proliferation of L929 cells was increased on membranes functionalized with EVs (p > 0.05).
AB - Bioactive material concepts for targeted therapy have been an important research focus in regenerative medicine for years. The aim of this study was to investigate a proof-of-concept composite structure in the form of a membrane made of natural silk fibroin (SF) and extracellular vesicles (EVs) from gingival fibroblasts. EVs have multiple abilities to act on their target cell and can thus play crucial roles in both physiology and regeneration. This study used pH neutral, degradable SF-based membranes, which have excellent cell- and tissue-specific properties, as the carrier material. The characterization of the vesicles showed a size range between 120 and 180 nm and a high expression of the usual EV markers (e.g. CD9, CD63 and CD81), measured by nanoparticle tracking analysis (NTA) and single-EV flow analysis (IFCM). An initial integration of the EVs into the membrane was analyzed using scanning and transmission electron microscopy (SEM and TEM) and vesicles were successfully detected, even if they were not homogeneously distributed in the membrane. Using direct and indirect tests, the cytocompatibility of the membranes with and without EVs could be proven and showed significant differences compared to the toxic control (p < 0.05). Additionally, proliferation of L929 cells was increased on membranes functionalized with EVs (p > 0.05).
U2 - 10.1038/s41598-024-54014-y
DO - 10.1038/s41598-024-54014-y
M3 - SCORING: Journal article
C2 - 38347108
VL - 14
SP - 3553
JO - SCI REP-UK
JF - SCI REP-UK
SN - 2045-2322
IS - 1
ER -
