DNA hypermethylation as a predictor of PSA recurrence in patients with low- and intermediate-grade prostate cancer
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DNA hypermethylation as a predictor of PSA recurrence in patients with low- and intermediate-grade prostate cancer. / Moritz, Rudolf; Ellinger, Jörg; Nuhn, Philipp; Haese, Alexander; Müller, Stefan C; Graefen, Markus; Schlomm, Thorsten; Bastian, Patrick J.
in: ANTICANCER RES, Jahrgang 33, Nr. 12, 01.12.2013, S. 5249-54.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - DNA hypermethylation as a predictor of PSA recurrence in patients with low- and intermediate-grade prostate cancer
AU - Moritz, Rudolf
AU - Ellinger, Jörg
AU - Nuhn, Philipp
AU - Haese, Alexander
AU - Müller, Stefan C
AU - Graefen, Markus
AU - Schlomm, Thorsten
AU - Bastian, Patrick J
PY - 2013/12/1
Y1 - 2013/12/1
N2 - BACKGROUND: DNA CpG island hypermethylation causes gene silencing and is a common event in prostate carcinogenesis and progression. We investigated its role as a possible prognostic marker in patients with PCA Gleason score ≤7.PATIENTS AND METHODS: We used a quantitative, methylation-specific PCR to analyze methylation patterns at five gene loci (APC, GSTP1, PTGS2, RARbeta and TIG1) in 84 prostate cancer (PCA) tissues (Gleason Score ≤7). Methylation was correlated with established clinico-pathological parameters (preoperative PSA, pathological Gleason score, extraprostatic extension, seminal vesicle penetration, lymph node involvement, surgical margins and age) and PSA recurrence.RESULTS: DNA hypermethylation was frequently detected at APC (95.2%), GSTP1 (84.5%), PTGS2 (100%), RAR-beta (81.0%) and TIG1 (95.2%). DNA hypermethylation was correlated with Gleason Score (p=0.027; PTGS2) and lymph node involvement (p=0.024; RARbeta). High methylation levels at RARbeta (p=0.023) was a significant predictor of PSA recurrence following radical prostatectomy.CONCLUSION: The analysis of DNA hypermethylation provides prognostic information in prognosis of low- and intermediate-grade PCA.
AB - BACKGROUND: DNA CpG island hypermethylation causes gene silencing and is a common event in prostate carcinogenesis and progression. We investigated its role as a possible prognostic marker in patients with PCA Gleason score ≤7.PATIENTS AND METHODS: We used a quantitative, methylation-specific PCR to analyze methylation patterns at five gene loci (APC, GSTP1, PTGS2, RARbeta and TIG1) in 84 prostate cancer (PCA) tissues (Gleason Score ≤7). Methylation was correlated with established clinico-pathological parameters (preoperative PSA, pathological Gleason score, extraprostatic extension, seminal vesicle penetration, lymph node involvement, surgical margins and age) and PSA recurrence.RESULTS: DNA hypermethylation was frequently detected at APC (95.2%), GSTP1 (84.5%), PTGS2 (100%), RAR-beta (81.0%) and TIG1 (95.2%). DNA hypermethylation was correlated with Gleason Score (p=0.027; PTGS2) and lymph node involvement (p=0.024; RARbeta). High methylation levels at RARbeta (p=0.023) was a significant predictor of PSA recurrence following radical prostatectomy.CONCLUSION: The analysis of DNA hypermethylation provides prognostic information in prognosis of low- and intermediate-grade PCA.
KW - CpG Islands
KW - DNA Methylation
KW - Humans
KW - Male
KW - Middle Aged
KW - Polymerase Chain Reaction
KW - Prostate-Specific Antigen
KW - Prostatic Neoplasms
M3 - SCORING: Journal article
C2 - 24324057
VL - 33
SP - 5249
EP - 5254
JO - ANTICANCER RES
JF - ANTICANCER RES
SN - 0250-7005
IS - 12
ER -