Diverging impact of cell fate determinants Scrib and Llgl1 on adhesion and migration of hematopoietic stem cells

TY - JOUR

T1 - Diverging impact of cell fate determinants Scrib and Llgl1 on adhesion and migration of hematopoietic stem cells

AU - Dash, Banaja P

AU - Schnöder, Tina M

AU - Kathner, Carolin

AU - Mohr, Juliane

AU - Weinert, Sönke

AU - Herzog, Carolin

AU - Godavarthy, Parimala Sonika

AU - Zanetti, Costanza

AU - Perner, Florian

AU - Braun-Dullaeus, Rüdiger

AU - Hartleben, Björn

AU - Huber, Tobias B

AU - Walz, Gerd

AU - Naumann, Michael

AU - Ellis, Sarah

AU - Vasioukhin, Valera

AU - Kähne, Thilo

AU - Krause, Daniela S

AU - Heidel, Florian H

PY - 2018/10

Y1 - 2018/10

N2 - PURPOSE: Cell fate determinants Scrib and Llgl1 influence self-renewal capacity of hematopoietic stem cells (HSCs). Scrib-deficient HSCs are functionally impaired and lack sufficient repopulation capacity during serial transplantation and stress. In contrast, loss of Llgl1 leads to increased HSC fitness, gain of self-renewal capacity and expansion of the stem cell pool. Here, we sought to assess for shared and unique molecular functions of Llgl1 and Scrib by analyzing their interactome in hematopoietic cells.METHODS: Interactome analysis was performed by affinity purification followed by mass spectrometry. Motility, migration and adhesion were assessed on primary murine HSCs, which were isolated by FACS sorting following conditional deletion of Scrib or Llgl1, respectively. Imaging of Scrib-deficient HSCs was performed by intravital 2-photon microscopy.RESULTS: Comparison of Scrib and Llgl1 interactome analyses revealed involvement in common and unique cellular functions. Migration and adhesion were among the cellular functions connected to Scrib but not to Llgl1. Functional validation of these findings confirmed alterations in cell adhesion and migration of Scrib-deficient HSCs in vitro and in vivo. In contrast, genetic inactivation of Llgl1 did not affect adhesion or migratory capacity of hematopoietic stem cells.CONCLUSION: Our data provide first evidence for an evolutionarily conserved role of the cell fate determinant Scrib in HSC adhesion and migration in vitro and in vivo, a unique function that is not shared with its putative complex partner Llgl1.

AB - PURPOSE: Cell fate determinants Scrib and Llgl1 influence self-renewal capacity of hematopoietic stem cells (HSCs). Scrib-deficient HSCs are functionally impaired and lack sufficient repopulation capacity during serial transplantation and stress. In contrast, loss of Llgl1 leads to increased HSC fitness, gain of self-renewal capacity and expansion of the stem cell pool. Here, we sought to assess for shared and unique molecular functions of Llgl1 and Scrib by analyzing their interactome in hematopoietic cells.METHODS: Interactome analysis was performed by affinity purification followed by mass spectrometry. Motility, migration and adhesion were assessed on primary murine HSCs, which were isolated by FACS sorting following conditional deletion of Scrib or Llgl1, respectively. Imaging of Scrib-deficient HSCs was performed by intravital 2-photon microscopy.RESULTS: Comparison of Scrib and Llgl1 interactome analyses revealed involvement in common and unique cellular functions. Migration and adhesion were among the cellular functions connected to Scrib but not to Llgl1. Functional validation of these findings confirmed alterations in cell adhesion and migration of Scrib-deficient HSCs in vitro and in vivo. In contrast, genetic inactivation of Llgl1 did not affect adhesion or migratory capacity of hematopoietic stem cells.CONCLUSION: Our data provide first evidence for an evolutionarily conserved role of the cell fate determinant Scrib in HSC adhesion and migration in vitro and in vivo, a unique function that is not shared with its putative complex partner Llgl1.

KW - Animals

KW - Apoptosis

KW - Cell Adhesion

KW - Cell Differentiation

KW - Cell Lineage

KW - Cell Movement

KW - Cell Proliferation

KW - Cells, Cultured

KW - Hematopoietic Stem Cells

KW - Homeodomain Proteins

KW - Intracellular Signaling Peptides and Proteins

KW - Mice

KW - Mice, Inbred C57BL

KW - Protein Interaction Domains and Motifs

KW - Proteome

KW - Tumor Suppressor Proteins

KW - Journal Article

U2 - 10.1007/s00432-018-2724-3

DO - 10.1007/s00432-018-2724-3

M3 - SCORING: Journal article

C2 - 30083817

VL - 144

SP - 1933

EP - 1944

JO - J CANCER RES CLIN

JF - J CANCER RES CLIN

SN - 0171-5216

IS - 10

ER -