Distribution of alarin immunoreactivity in the mouse brain.
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Distribution of alarin immunoreactivity in the mouse brain. / Eberhard, Nicole; Mayer, Christian; Santic, Radmila; Navio, Ruben Peco; Wagner, Andrea; Bauer, Hans Christian; Sperk, Guenther; Boehm, Ulrich; Kofler, Barbara.
in: J MOL NEUROSCI, Jahrgang 46, Nr. 1, 1, 2012, S. 18-32.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Distribution of alarin immunoreactivity in the mouse brain.
AU - Eberhard, Nicole
AU - Mayer, Christian
AU - Santic, Radmila
AU - Navio, Ruben Peco
AU - Wagner, Andrea
AU - Bauer, Hans Christian
AU - Sperk, Guenther
AU - Boehm, Ulrich
AU - Kofler, Barbara
PY - 2012
Y1 - 2012
N2 - Alarin is a 25 amino acid peptide that belongs to the galanin peptide family. It is derived from the galanin-like peptide gene by a splice variant, which excludes exon 3. Alarin was first identified in gangliocytes of neuroblastic tumors and later shown to have a vasoactive function in the skin. Recently, alarin was demonstrated to stimulate food intake as well as the hypothalamic-pituitary-gonadal axis in rodents, suggesting that it might be a neuromodulatory peptide in the brain. However, the individual neurons in the central nervous system that express alarin have not been identified. Here, we determined the distribution of alarin-like immunoreactivity (alarin-LI) in the adult murine brain. The specificity of the antibody against alarin was demonstrated by the absence of labeling after pre-absorption of the antiserum with synthetic alarin peptide and in transgenic mouse brains lacking neurons expressing the GALP gene. Alarin-LI was observed in different areas of the murine brain. A high intensity of alarin-LI was detected in the accessory olfactory bulb, the medial preoptic area, the amygdala, different nuclei of the hypothalamus such as the arcuate nucleus and the ventromedial hypothalamic nucleus, the trigeminal complex, the locus coeruleus, the ventral chochlear nucleus, the facial nucleus, and the epithelial layer of the plexus choroideus. The distinct expression pattern of alarin in the adult mouse brain suggests potential functions in reproduction and metabolism.
AB - Alarin is a 25 amino acid peptide that belongs to the galanin peptide family. It is derived from the galanin-like peptide gene by a splice variant, which excludes exon 3. Alarin was first identified in gangliocytes of neuroblastic tumors and later shown to have a vasoactive function in the skin. Recently, alarin was demonstrated to stimulate food intake as well as the hypothalamic-pituitary-gonadal axis in rodents, suggesting that it might be a neuromodulatory peptide in the brain. However, the individual neurons in the central nervous system that express alarin have not been identified. Here, we determined the distribution of alarin-like immunoreactivity (alarin-LI) in the adult murine brain. The specificity of the antibody against alarin was demonstrated by the absence of labeling after pre-absorption of the antiserum with synthetic alarin peptide and in transgenic mouse brains lacking neurons expressing the GALP gene. Alarin-LI was observed in different areas of the murine brain. A high intensity of alarin-LI was detected in the accessory olfactory bulb, the medial preoptic area, the amygdala, different nuclei of the hypothalamus such as the arcuate nucleus and the ventromedial hypothalamic nucleus, the trigeminal complex, the locus coeruleus, the ventral chochlear nucleus, the facial nucleus, and the epithelial layer of the plexus choroideus. The distinct expression pattern of alarin in the adult mouse brain suggests potential functions in reproduction and metabolism.
KW - Animals
KW - Age Factors
KW - Mice
KW - Mice, Inbred C57BL
KW - Rabbits
KW - Mice, Transgenic
KW - Alternative Splicing/genetics/immunology
KW - Antigen-Antibody Reactions/genetics/immunology
KW - Brain Chemistry/genetics/immunology
KW - Galanin-Like Peptide/genetics/immunology/metabolism
KW - Neuropeptides/genetics/immunology/metabolism
KW - Tissue Distribution/genetics/immunology
KW - Animals
KW - Age Factors
KW - Mice
KW - Mice, Inbred C57BL
KW - Rabbits
KW - Mice, Transgenic
KW - Alternative Splicing/genetics/immunology
KW - Antigen-Antibody Reactions/genetics/immunology
KW - Brain Chemistry/genetics/immunology
KW - Galanin-Like Peptide/genetics/immunology/metabolism
KW - Neuropeptides/genetics/immunology/metabolism
KW - Tissue Distribution/genetics/immunology
M3 - SCORING: Journal article
VL - 46
SP - 18
EP - 32
JO - J MOL NEUROSCI
JF - J MOL NEUROSCI
SN - 0895-8696
IS - 1
M1 - 1
ER -
