Disentangling the Roles of RIM and Munc13 in Synaptic Vesicle Localization and Neurotransmission
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Disentangling the Roles of RIM and Munc13 in Synaptic Vesicle Localization and Neurotransmission. / Zarebidaki, Fereshteh; Camacho, Marcial; Brockmann, Marisa M.; Trimbuch, Thorsten; Herman, Melissa A.; Rosenmund, Christian.
in: J NEUROSCI, Jahrgang 40, Nr. 49, 02.12.2020, S. 9372-9385.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Disentangling the Roles of RIM and Munc13 in Synaptic Vesicle Localization and Neurotransmission
AU - Zarebidaki, Fereshteh
AU - Camacho, Marcial
AU - Brockmann, Marisa M.
AU - Trimbuch, Thorsten
AU - Herman, Melissa A.
AU - Rosenmund, Christian
PY - 2020/12/2
Y1 - 2020/12/2
N2 - Efficient neurotransmitter release at the presynaptic terminal requires docking of synaptic vesicles to the active zone membrane and formation of fusion-competent synaptic vesicles near voltage-gated Ca2+ channels. Rab3-interacting molecule (RIM) is a critical active zone organizer, as it recruits Ca2+ channels and activates synaptic vesicle docking and priming via Munc13-1. However, our knowledge about Munc13-independent contributions of RIM to active zone functions is limited. To identify the functions that are solely mediated by RIM, we used genetic manipulations to control RIM and Munc13-1 activity in cultured hippocampal neurons from mice of either sex and compared synaptic ultrastructure and neurotransmission. We found that RIM modulates synaptic vesicle localization in the proximity of the active zone membrane independent of Munc13-1. In another step, both RIM and Munc13 mediate synaptic vesicle docking and priming. In addition, while the activity of both RIM and Munc13-1 is required for Ca2+-evoked release, RIM uniquely controls neurotransmitter release efficiency. However, activity-dependent augmentation of synaptic vesicle pool size relies exclusively on the action of Munc13s. Based on our results, we extend previous findings and propose a refined model in which RIM and Munc13-1 act in overlapping and independent stages of synaptic vesicle localization and release.
AB - Efficient neurotransmitter release at the presynaptic terminal requires docking of synaptic vesicles to the active zone membrane and formation of fusion-competent synaptic vesicles near voltage-gated Ca2+ channels. Rab3-interacting molecule (RIM) is a critical active zone organizer, as it recruits Ca2+ channels and activates synaptic vesicle docking and priming via Munc13-1. However, our knowledge about Munc13-independent contributions of RIM to active zone functions is limited. To identify the functions that are solely mediated by RIM, we used genetic manipulations to control RIM and Munc13-1 activity in cultured hippocampal neurons from mice of either sex and compared synaptic ultrastructure and neurotransmission. We found that RIM modulates synaptic vesicle localization in the proximity of the active zone membrane independent of Munc13-1. In another step, both RIM and Munc13 mediate synaptic vesicle docking and priming. In addition, while the activity of both RIM and Munc13-1 is required for Ca2+-evoked release, RIM uniquely controls neurotransmitter release efficiency. However, activity-dependent augmentation of synaptic vesicle pool size relies exclusively on the action of Munc13s. Based on our results, we extend previous findings and propose a refined model in which RIM and Munc13-1 act in overlapping and independent stages of synaptic vesicle localization and release.
U2 - 10.1523/JNEUROSCI.1922-20.2020
DO - 10.1523/JNEUROSCI.1922-20.2020
M3 - SCORING: Journal article
VL - 40
SP - 9372
EP - 9385
JO - J NEUROSCI
JF - J NEUROSCI
SN - 0270-6474
IS - 49
ER -