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Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes

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Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes. / Haftmann, Claudia; Riedel, René; Porstner, Martina; Wittmann, Jürgen; Chang, Hyun-Dong; Radbruch, Andreas; Mashreghi, Mir-Farzin.

in: J IMMUNOL METHODS, Jahrgang 426, 11.2015, S. 128-33.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Haftmann, C, Riedel, R, Porstner, M, Wittmann, J, Chang, H-D, Radbruch, A & Mashreghi, M-F 2015, 'Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes', J IMMUNOL METHODS, Jg. 426, S. 128-33. https://doi.org/10.1016/j.jim.2015.07.006

APA

Haftmann, C., Riedel, R., Porstner, M., Wittmann, J., Chang, H-D., Radbruch, A., & Mashreghi, M-F. (2015). Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes. J IMMUNOL METHODS, 426, 128-33. https://doi.org/10.1016/j.jim.2015.07.006

Vancouver

Haftmann C, Riedel R, Porstner M, Wittmann J, Chang H-D, Radbruch A et al. Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes. J IMMUNOL METHODS. 2015 Nov;426:128-33. https://doi.org/10.1016/j.jim.2015.07.006

Bibtex

@article{e4bee81573a54e239a25938479ce9140,
title = "Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes",
abstract = "Depending on their physiological expression level, microRNAs (miRNA) address different target genes, thus have different biological functions. To identify these, the physiological expression has to be blocked. Here, we describe the use of inhibitory cholesterol-modified oligonucleotides (Antagomirs) to inhibit miRNAs selectively in primary human and murine T and B lymphocytes. Due to their lipophilic cholesterol tag Antagomirs enter primary lymphocytes efficiently and quantitatively. We show here that at concentrations of 0.125 to 1μM, Antagomirs selectively inhibit expression of their target miRNA up to 99.5% without affecting cell viability. ",
keywords = "Animals, B-Lymphocytes/immunology, Biological Transport, Cells, Cultured, Gene Knockdown Techniques, Humans, Mice, Mice, Inbred C57BL, MicroRNAs/antagonists & inhibitors, Oligonucleotides/genetics, Spleen/cytology, Th1 Cells/immunology",
author = "Claudia Haftmann and Ren{\'e} Riedel and Martina Porstner and J{\"u}rgen Wittmann and Hyun-Dong Chang and Andreas Radbruch and Mir-Farzin Mashreghi",
note = "Copyright {\textcopyright} 2015. Published by Elsevier B.V.",
year = "2015",
month = nov,
doi = "10.1016/j.jim.2015.07.006",
language = "English",
volume = "426",
pages = "128--33",
journal = "J IMMUNOL METHODS",
issn = "0022-1759",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Direct uptake of Antagomirs and efficient knockdown of miRNA in primary B and T lymphocytes

AU - Haftmann, Claudia

AU - Riedel, René

AU - Porstner, Martina

AU - Wittmann, Jürgen

AU - Chang, Hyun-Dong

AU - Radbruch, Andreas

AU - Mashreghi, Mir-Farzin

N1 - Copyright © 2015. Published by Elsevier B.V.

PY - 2015/11

Y1 - 2015/11

N2 - Depending on their physiological expression level, microRNAs (miRNA) address different target genes, thus have different biological functions. To identify these, the physiological expression has to be blocked. Here, we describe the use of inhibitory cholesterol-modified oligonucleotides (Antagomirs) to inhibit miRNAs selectively in primary human and murine T and B lymphocytes. Due to their lipophilic cholesterol tag Antagomirs enter primary lymphocytes efficiently and quantitatively. We show here that at concentrations of 0.125 to 1μM, Antagomirs selectively inhibit expression of their target miRNA up to 99.5% without affecting cell viability.

AB - Depending on their physiological expression level, microRNAs (miRNA) address different target genes, thus have different biological functions. To identify these, the physiological expression has to be blocked. Here, we describe the use of inhibitory cholesterol-modified oligonucleotides (Antagomirs) to inhibit miRNAs selectively in primary human and murine T and B lymphocytes. Due to their lipophilic cholesterol tag Antagomirs enter primary lymphocytes efficiently and quantitatively. We show here that at concentrations of 0.125 to 1μM, Antagomirs selectively inhibit expression of their target miRNA up to 99.5% without affecting cell viability.

KW - Animals

KW - B-Lymphocytes/immunology

KW - Biological Transport

KW - Cells, Cultured

KW - Gene Knockdown Techniques

KW - Humans

KW - Mice

KW - Mice, Inbred C57BL

KW - MicroRNAs/antagonists & inhibitors

KW - Oligonucleotides/genetics

KW - Spleen/cytology

KW - Th1 Cells/immunology

U2 - 10.1016/j.jim.2015.07.006

DO - 10.1016/j.jim.2015.07.006

M3 - SCORING: Journal article

C2 - 26187895

VL - 426

SP - 128

EP - 133

JO - J IMMUNOL METHODS

JF - J IMMUNOL METHODS

SN - 0022-1759

ER -