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Direct current stimulation-induced synaptic plasticity in the sensorimotor cortex: structure follows function

Standard

Direct current stimulation-induced synaptic plasticity in the sensorimotor cortex: structure follows function. / Gellner, Anne-Kathrin; Reis, Janine; Holtick, Carsten; Schubert, Charlotte; Fritsch, Brita.

in: BRAIN STIMUL, Jahrgang 13, Nr. 1, 14.08.2019, S. 80-88.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Gellner, A-K, Reis, J, Holtick, C, Schubert, C & Fritsch, B 2019, 'Direct current stimulation-induced synaptic plasticity in the sensorimotor cortex: structure follows function', BRAIN STIMUL, Jg. 13, Nr. 1, S. 80-88. https://doi.org/10.1016/j.brs.2019.07.026

APA

Gellner, A-K., Reis, J., Holtick, C., Schubert, C., & Fritsch, B. (2019). Direct current stimulation-induced synaptic plasticity in the sensorimotor cortex: structure follows function. BRAIN STIMUL, 13(1), 80-88. https://doi.org/10.1016/j.brs.2019.07.026

Vancouver

Gellner A-K, Reis J, Holtick C, Schubert C, Fritsch B. Direct current stimulation-induced synaptic plasticity in the sensorimotor cortex: structure follows function. BRAIN STIMUL. 2019 Aug 14;13(1):80-88. https://doi.org/10.1016/j.brs.2019.07.026

Bibtex

@article{1fdf2d71bfff4e97a4fb6edb2dbb8c3c,
title = "Direct current stimulation-induced synaptic plasticity in the sensorimotor cortex: structure follows function",
abstract = "BACKGROUND: Non-invasive direct current stimulation (DCS) of the brain induces functional plasticity in vitro and facilitates motor learning across species. The effect of DCS on structural synaptic plasticity is currently unknown.OBJECTIVE: This study addresses the effects and the underlying mechanisms of anodal DCS on structural plasticity and morphology of dendritic spines in the sensorimotor cortex (M1/S1).METHODS: A DCS electrode setup was combined with a chronic cranial window over M1/S1 in transgenic Thy1-GFP mice, to allow for in vivo 2-photon microscopy and simultaneous DCS. Contralateral electrical forepaw stimulation (eFS) was used to mimic the second synapse specific input, a previously shown requirement to induce functional plasticity by DCS. Changes in spine density and spine morphology were compared between DCS/eFS and sham, as well as two control conditions (sham-DCS/eFS, DCS/sham-eFS). Furthermore, the role of BDNF for stimulation-induced changes in spine density was assessed in heterozygous Thy1-GFP x BDNF+/- mice.RESULTS: Combined DCS/eFS rapidly increased spine density during stimulation and changes outlasted the intervention for 24 h. This effect was due to increased survival of original spines and a preferential formation of new spines after intervention. The latter were morphologically characterized by larger head sizes. The DCS-induced spine density increase was absent in mice with reduced BDNF expression.CONCLUSION: Previous findings of DCS-induced functional synaptic plasticity can be extended to structural plasticity in M1/S1 that similarly depends on a second synaptic input (eFS) and requires physiological BDNF expression. These findings show considerable parallels to motor learning-induced M1 spine dynamics.",
author = "Anne-Kathrin Gellner and Janine Reis and Carsten Holtick and Charlotte Schubert and Brita Fritsch",
note = "Copyright {\textcopyright} 2019. Published by Elsevier Inc.",
year = "2019",
month = aug,
day = "14",
doi = "10.1016/j.brs.2019.07.026",
language = "English",
volume = "13",
pages = "80--88",
journal = "BRAIN STIMUL",
issn = "1935-861X",
publisher = "ELSEVIER SCIENCE INC",
number = "1",

}

RIS

TY - JOUR

T1 - Direct current stimulation-induced synaptic plasticity in the sensorimotor cortex: structure follows function

AU - Gellner, Anne-Kathrin

AU - Reis, Janine

AU - Holtick, Carsten

AU - Schubert, Charlotte

AU - Fritsch, Brita

N1 - Copyright © 2019. Published by Elsevier Inc.

PY - 2019/8/14

Y1 - 2019/8/14

N2 - BACKGROUND: Non-invasive direct current stimulation (DCS) of the brain induces functional plasticity in vitro and facilitates motor learning across species. The effect of DCS on structural synaptic plasticity is currently unknown.OBJECTIVE: This study addresses the effects and the underlying mechanisms of anodal DCS on structural plasticity and morphology of dendritic spines in the sensorimotor cortex (M1/S1).METHODS: A DCS electrode setup was combined with a chronic cranial window over M1/S1 in transgenic Thy1-GFP mice, to allow for in vivo 2-photon microscopy and simultaneous DCS. Contralateral electrical forepaw stimulation (eFS) was used to mimic the second synapse specific input, a previously shown requirement to induce functional plasticity by DCS. Changes in spine density and spine morphology were compared between DCS/eFS and sham, as well as two control conditions (sham-DCS/eFS, DCS/sham-eFS). Furthermore, the role of BDNF for stimulation-induced changes in spine density was assessed in heterozygous Thy1-GFP x BDNF+/- mice.RESULTS: Combined DCS/eFS rapidly increased spine density during stimulation and changes outlasted the intervention for 24 h. This effect was due to increased survival of original spines and a preferential formation of new spines after intervention. The latter were morphologically characterized by larger head sizes. The DCS-induced spine density increase was absent in mice with reduced BDNF expression.CONCLUSION: Previous findings of DCS-induced functional synaptic plasticity can be extended to structural plasticity in M1/S1 that similarly depends on a second synaptic input (eFS) and requires physiological BDNF expression. These findings show considerable parallels to motor learning-induced M1 spine dynamics.

AB - BACKGROUND: Non-invasive direct current stimulation (DCS) of the brain induces functional plasticity in vitro and facilitates motor learning across species. The effect of DCS on structural synaptic plasticity is currently unknown.OBJECTIVE: This study addresses the effects and the underlying mechanisms of anodal DCS on structural plasticity and morphology of dendritic spines in the sensorimotor cortex (M1/S1).METHODS: A DCS electrode setup was combined with a chronic cranial window over M1/S1 in transgenic Thy1-GFP mice, to allow for in vivo 2-photon microscopy and simultaneous DCS. Contralateral electrical forepaw stimulation (eFS) was used to mimic the second synapse specific input, a previously shown requirement to induce functional plasticity by DCS. Changes in spine density and spine morphology were compared between DCS/eFS and sham, as well as two control conditions (sham-DCS/eFS, DCS/sham-eFS). Furthermore, the role of BDNF for stimulation-induced changes in spine density was assessed in heterozygous Thy1-GFP x BDNF+/- mice.RESULTS: Combined DCS/eFS rapidly increased spine density during stimulation and changes outlasted the intervention for 24 h. This effect was due to increased survival of original spines and a preferential formation of new spines after intervention. The latter were morphologically characterized by larger head sizes. The DCS-induced spine density increase was absent in mice with reduced BDNF expression.CONCLUSION: Previous findings of DCS-induced functional synaptic plasticity can be extended to structural plasticity in M1/S1 that similarly depends on a second synaptic input (eFS) and requires physiological BDNF expression. These findings show considerable parallels to motor learning-induced M1 spine dynamics.

U2 - 10.1016/j.brs.2019.07.026

DO - 10.1016/j.brs.2019.07.026

M3 - SCORING: Journal article

C2 - 31405790

VL - 13

SP - 80

EP - 88

JO - BRAIN STIMUL

JF - BRAIN STIMUL

SN - 1935-861X

IS - 1

ER -