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Dinucleotides as growth-promoting extracellular mediators. Presence of dinucleoside diphosphates Ap2A, Ap2G, and Gp2G in releasable granules of platelets

Standard

Dinucleotides as growth-promoting extracellular mediators. Presence of dinucleoside diphosphates Ap2A, Ap2G, and Gp2G in releasable granules of platelets. / Jankowski, J; Hagemann, J; Tepel, M; van Der Giet, M; Stephan, N; Henning, L; Gouni-Berthold, I; Sachinidis, A; Zidek, W; Schluter, H; Gouni-Berthold, H.

in: J BIOL CHEM, Jahrgang 276, Nr. 12, 23.03.2001, S. 8904-9.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Jankowski, J, Hagemann, J, Tepel, M, van Der Giet, M, Stephan, N, Henning, L, Gouni-Berthold, I, Sachinidis, A, Zidek, W, Schluter, H & Gouni-Berthold, H 2001, 'Dinucleotides as growth-promoting extracellular mediators. Presence of dinucleoside diphosphates Ap2A, Ap2G, and Gp2G in releasable granules of platelets', J BIOL CHEM, Jg. 276, Nr. 12, S. 8904-9. https://doi.org/10.1074/jbc.M009527200

APA

Jankowski, J., Hagemann, J., Tepel, M., van Der Giet, M., Stephan, N., Henning, L., Gouni-Berthold, I., Sachinidis, A., Zidek, W., Schluter, H., & Gouni-Berthold, H. (2001). Dinucleotides as growth-promoting extracellular mediators. Presence of dinucleoside diphosphates Ap2A, Ap2G, and Gp2G in releasable granules of platelets. J BIOL CHEM, 276(12), 8904-9. https://doi.org/10.1074/jbc.M009527200

Vancouver

Jankowski J, Hagemann J, Tepel M, van Der Giet M, Stephan N, Henning L et al. Dinucleotides as growth-promoting extracellular mediators. Presence of dinucleoside diphosphates Ap2A, Ap2G, and Gp2G in releasable granules of platelets. J BIOL CHEM. 2001 Mär 23;276(12):8904-9. https://doi.org/10.1074/jbc.M009527200

Bibtex

@article{163c2e1b3aed4b71b1d5c95653852290,
title = "Dinucleotides as growth-promoting extracellular mediators. Presence of dinucleoside diphosphates Ap2A, Ap2G, and Gp2G in releasable granules of platelets",
abstract = "Dinucleoside diphosphates, Ap(2)A, Ap(2)G, and Gp(2)G represent a new class of growth-promoting extracellular mediators, which are released from granules after activation of platelets. The presence of theses substances was shown after purification from a platelet concentrate. The substances were identified by UV spectrometry, retention time comparison with authentic substances, matrix-assisted laser desorption/ionization mass spectrometry, post-source-decay matrix-assisted laser desorption/ionization mass spectrometry, and enzymatic analysis. Ap(2)A, Ap(2)G, and Gp(2)G have growth-stimulating effects on vascular smooth muscle cells in nanomolar concentrations as shown by [(3)H]thymidine incorporation measurements. The calculated EC(50) (log m; mean +/- S.E.) values were -6.07 +/- 0.14 for Ap(2)A, -6.27 +/- 0.25 for Ap(2)G, and -6.91 +/- 0.44 for Gp(2)G. At least 61.5 +/- 4.3% of the dinucleoside polyphosphates are released by platelet activation. The intraplatelet concentrations suggest that, in the close environment of a platelet thrombus, similar dinucleoside polyphosphate concentrations can be found as in platelets. Intraplatelet concentration can be estimated in the range of 1/20 to 1/100 of the concentration of ATP. In conclusion, Ap(2)A, Ap(2)G, and Gp(2)G derived from releasable granules of human platelets may play a regulatory role in vascular smooth muscle growth as growth-promoting mediators.",
keywords = "Animals, Blood Platelets, Cell Division, Chromatography, Liquid, Growth Substances, Humans, Muscle, Smooth, Vascular, Oligonucleotides, Platelet Activation, Rats, Rats, Inbred WKY, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Thrombin, Journal Article, Research Support, Non-U.S. Gov't",
author = "J Jankowski and J Hagemann and M Tepel and {van Der Giet}, M and N Stephan and L Henning and I Gouni-Berthold and A Sachinidis and W Zidek and H Schluter and H Gouni-Berthold",
year = "2001",
month = mar,
day = "23",
doi = "10.1074/jbc.M009527200",
language = "English",
volume = "276",
pages = "8904--9",
journal = "J BIOL CHEM",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "12",

}

RIS

TY - JOUR

T1 - Dinucleotides as growth-promoting extracellular mediators. Presence of dinucleoside diphosphates Ap2A, Ap2G, and Gp2G in releasable granules of platelets

AU - Jankowski, J

AU - Hagemann, J

AU - Tepel, M

AU - van Der Giet, M

AU - Stephan, N

AU - Henning, L

AU - Gouni-Berthold, I

AU - Sachinidis, A

AU - Zidek, W

AU - Schluter, H

AU - Gouni-Berthold, H

PY - 2001/3/23

Y1 - 2001/3/23

N2 - Dinucleoside diphosphates, Ap(2)A, Ap(2)G, and Gp(2)G represent a new class of growth-promoting extracellular mediators, which are released from granules after activation of platelets. The presence of theses substances was shown after purification from a platelet concentrate. The substances were identified by UV spectrometry, retention time comparison with authentic substances, matrix-assisted laser desorption/ionization mass spectrometry, post-source-decay matrix-assisted laser desorption/ionization mass spectrometry, and enzymatic analysis. Ap(2)A, Ap(2)G, and Gp(2)G have growth-stimulating effects on vascular smooth muscle cells in nanomolar concentrations as shown by [(3)H]thymidine incorporation measurements. The calculated EC(50) (log m; mean +/- S.E.) values were -6.07 +/- 0.14 for Ap(2)A, -6.27 +/- 0.25 for Ap(2)G, and -6.91 +/- 0.44 for Gp(2)G. At least 61.5 +/- 4.3% of the dinucleoside polyphosphates are released by platelet activation. The intraplatelet concentrations suggest that, in the close environment of a platelet thrombus, similar dinucleoside polyphosphate concentrations can be found as in platelets. Intraplatelet concentration can be estimated in the range of 1/20 to 1/100 of the concentration of ATP. In conclusion, Ap(2)A, Ap(2)G, and Gp(2)G derived from releasable granules of human platelets may play a regulatory role in vascular smooth muscle growth as growth-promoting mediators.

AB - Dinucleoside diphosphates, Ap(2)A, Ap(2)G, and Gp(2)G represent a new class of growth-promoting extracellular mediators, which are released from granules after activation of platelets. The presence of theses substances was shown after purification from a platelet concentrate. The substances were identified by UV spectrometry, retention time comparison with authentic substances, matrix-assisted laser desorption/ionization mass spectrometry, post-source-decay matrix-assisted laser desorption/ionization mass spectrometry, and enzymatic analysis. Ap(2)A, Ap(2)G, and Gp(2)G have growth-stimulating effects on vascular smooth muscle cells in nanomolar concentrations as shown by [(3)H]thymidine incorporation measurements. The calculated EC(50) (log m; mean +/- S.E.) values were -6.07 +/- 0.14 for Ap(2)A, -6.27 +/- 0.25 for Ap(2)G, and -6.91 +/- 0.44 for Gp(2)G. At least 61.5 +/- 4.3% of the dinucleoside polyphosphates are released by platelet activation. The intraplatelet concentrations suggest that, in the close environment of a platelet thrombus, similar dinucleoside polyphosphate concentrations can be found as in platelets. Intraplatelet concentration can be estimated in the range of 1/20 to 1/100 of the concentration of ATP. In conclusion, Ap(2)A, Ap(2)G, and Gp(2)G derived from releasable granules of human platelets may play a regulatory role in vascular smooth muscle growth as growth-promoting mediators.

KW - Animals

KW - Blood Platelets

KW - Cell Division

KW - Chromatography, Liquid

KW - Growth Substances

KW - Humans

KW - Muscle, Smooth, Vascular

KW - Oligonucleotides

KW - Platelet Activation

KW - Rats

KW - Rats, Inbred WKY

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Thrombin

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1074/jbc.M009527200

DO - 10.1074/jbc.M009527200

M3 - SCORING: Journal article

C2 - 11115507

VL - 276

SP - 8904

EP - 8909

JO - J BIOL CHEM

JF - J BIOL CHEM

SN - 0021-9258

IS - 12

ER -