Differentiation of Streptococcus pneumoniae from nonpneumococcal streptococci of the Streptococcus mitis group by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
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Differentiation of Streptococcus pneumoniae from nonpneumococcal streptococci of the Streptococcus mitis group by matrix-assisted laser desorption ionization-time of flight mass spectrometry. / Werno, Anja M; Christner, Martin; Anderson, Trevor P; Murdoch, David R.
in: J CLIN MICROBIOL, Jahrgang 50, Nr. 9, 9, 2012, S. 2863-2867.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Differentiation of Streptococcus pneumoniae from nonpneumococcal streptococci of the Streptococcus mitis group by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
AU - Werno, Anja M
AU - Christner, Martin
AU - Anderson, Trevor P
AU - Murdoch, David R
PY - 2012
Y1 - 2012
N2 - The differentiation of species within the Streptococcus mitis group has posed a problem in the routine diagnostic microbiology laboratory for some time. It also constitutes a major weakness of recently introduced matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) fingerprinting systems. As the phylogenetic resolution of the spectral similarity measures employed by these systems is insufficient to reliably distinguish between the most closely related members of the group, the major pathogen Streptococcus pneumoniae is frequently misidentified. In this study, a comparative analysis of MALDI-TOF spectra of several species from the S. mitis group has been performed in order to identify single peaks that could be used to improve mass spectrometry-based identification of the respective species. A characteristic peak profile could be identified that unambiguously distinguished the 14 S. pneumoniae isolates studied from 33 nonpneumococcal isolates of the S. mitis group. In addition, specific peak combinations could be assigned to other members of the group. The findings of this study suggest that it is possible to distinguish different species of the S. mitis group by close analysis of their mass peak profiles.
AB - The differentiation of species within the Streptococcus mitis group has posed a problem in the routine diagnostic microbiology laboratory for some time. It also constitutes a major weakness of recently introduced matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) fingerprinting systems. As the phylogenetic resolution of the spectral similarity measures employed by these systems is insufficient to reliably distinguish between the most closely related members of the group, the major pathogen Streptococcus pneumoniae is frequently misidentified. In this study, a comparative analysis of MALDI-TOF spectra of several species from the S. mitis group has been performed in order to identify single peaks that could be used to improve mass spectrometry-based identification of the respective species. A characteristic peak profile could be identified that unambiguously distinguished the 14 S. pneumoniae isolates studied from 33 nonpneumococcal isolates of the S. mitis group. In addition, specific peak combinations could be assigned to other members of the group. The findings of this study suggest that it is possible to distinguish different species of the S. mitis group by close analysis of their mass peak profiles.
KW - Humans
KW - Molecular Sequence Data
KW - Sequence Analysis, DNA
KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
KW - Bacteriological Techniques/methods
KW - DNA, Bacterial/chemistry/genetics
KW - Streptococcal Infections/diagnosis/microbiology
KW - Streptococcus/chemistry/classification/isolation & purification
KW - Humans
KW - Molecular Sequence Data
KW - Sequence Analysis, DNA
KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
KW - Bacteriological Techniques/methods
KW - DNA, Bacterial/chemistry/genetics
KW - Streptococcal Infections/diagnosis/microbiology
KW - Streptococcus/chemistry/classification/isolation & purification
M3 - SCORING: Journal article
VL - 50
SP - 2863
EP - 2867
JO - J CLIN MICROBIOL
JF - J CLIN MICROBIOL
SN - 0095-1137
IS - 9
M1 - 9
ER -
