Differential role of nicotinamide adenine dinucleotide deficiency in acute and chronic kidney disease
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Differential role of nicotinamide adenine dinucleotide deficiency in acute and chronic kidney disease. / Faivre, Anna; Katsyuba, Elena; Verissimo, Thomas; Lindenmeyer, Maja; Rajaram, Renuga Devi; Naesens, Maarten; Heckenmeyer, Carolyn; Mottis, Adrienne; Feraille, Eric; Cippà, Pietro; Cohen, Clemens; Longchamp, Alban; Allagnat, Florent; Rutkowski, Joseph M; Legouis, David; Auwerx, Johan; de Seigneux, Sophie.
in: NEPHROL DIAL TRANSPL, Jahrgang 36, Nr. 1, 01.01.2021, S. 60-68.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Differential role of nicotinamide adenine dinucleotide deficiency in acute and chronic kidney disease
AU - Faivre, Anna
AU - Katsyuba, Elena
AU - Verissimo, Thomas
AU - Lindenmeyer, Maja
AU - Rajaram, Renuga Devi
AU - Naesens, Maarten
AU - Heckenmeyer, Carolyn
AU - Mottis, Adrienne
AU - Feraille, Eric
AU - Cippà, Pietro
AU - Cohen, Clemens
AU - Longchamp, Alban
AU - Allagnat, Florent
AU - Rutkowski, Joseph M
AU - Legouis, David
AU - Auwerx, Johan
AU - de Seigneux, Sophie
N1 - © The Author(s) 2020. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved.
PY - 2021/1/1
Y1 - 2021/1/1
N2 - BACKGROUND: Nicotinamide adenine dinucleotide (NAD+) is a ubiquitous coenzyme involved in electron transport and a co-substrate for sirtuin function. NAD+ deficiency has been demonstrated in the context of acute kidney injury (AKI).METHODS: We studied the expression of key NAD+ biosynthesis enzymes in kidney biopsies from human allograft patients and patients with chronic kidney disease (CKD) at different stages. We used ischaemia-reperfusion injury (IRI) and cisplatin injection to model AKI, urinary tract obstruction [unilateral ureteral obstruction (UUO)] and tubulointerstitial fibrosis induced by proteinuria to investigate CKD in mice. We assessed the effect of nicotinamide riboside (NR) supplementation on AKI and CKD in animal models.RESULTS: RNA sequencing analysis of human kidney allograft biopsies during the reperfusion phase showed that the NAD+ de novo synthesis is impaired in the immediate post-transplantation period, whereas the salvage pathway is stimulated. This decrease in de novo NAD+ synthesis was confirmed in two mouse models of IRI where NR supplementation prevented plasma urea and creatinine elevation and tubular injury. In human biopsies from CKD patients, the NAD+ de novo synthesis pathway was impaired according to CKD stage, with better preservation of the salvage pathway. Similar alterations in gene expression were observed in mice with UUO or chronic proteinuric glomerular disease. NR supplementation did not prevent CKD progression, in contrast to its efficacy in AKI.CONCLUSION: Impairment of NAD+ synthesis is a hallmark of AKI and CKD. NR supplementation is beneficial in ischaemic AKI but not in CKD models.
AB - BACKGROUND: Nicotinamide adenine dinucleotide (NAD+) is a ubiquitous coenzyme involved in electron transport and a co-substrate for sirtuin function. NAD+ deficiency has been demonstrated in the context of acute kidney injury (AKI).METHODS: We studied the expression of key NAD+ biosynthesis enzymes in kidney biopsies from human allograft patients and patients with chronic kidney disease (CKD) at different stages. We used ischaemia-reperfusion injury (IRI) and cisplatin injection to model AKI, urinary tract obstruction [unilateral ureteral obstruction (UUO)] and tubulointerstitial fibrosis induced by proteinuria to investigate CKD in mice. We assessed the effect of nicotinamide riboside (NR) supplementation on AKI and CKD in animal models.RESULTS: RNA sequencing analysis of human kidney allograft biopsies during the reperfusion phase showed that the NAD+ de novo synthesis is impaired in the immediate post-transplantation period, whereas the salvage pathway is stimulated. This decrease in de novo NAD+ synthesis was confirmed in two mouse models of IRI where NR supplementation prevented plasma urea and creatinine elevation and tubular injury. In human biopsies from CKD patients, the NAD+ de novo synthesis pathway was impaired according to CKD stage, with better preservation of the salvage pathway. Similar alterations in gene expression were observed in mice with UUO or chronic proteinuric glomerular disease. NR supplementation did not prevent CKD progression, in contrast to its efficacy in AKI.CONCLUSION: Impairment of NAD+ synthesis is a hallmark of AKI and CKD. NR supplementation is beneficial in ischaemic AKI but not in CKD models.
U2 - 10.1093/ndt/gfaa124
DO - 10.1093/ndt/gfaa124
M3 - SCORING: Journal article
C2 - 33099633
VL - 36
SP - 60
EP - 68
JO - NEPHROL DIAL TRANSPL
JF - NEPHROL DIAL TRANSPL
SN - 0931-0509
IS - 1
ER -