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Differential marker protein expression specifies rarefaction zone-containing human A (dark) spermatogonia.

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Differential marker protein expression specifies rarefaction zone-containing human A (dark) spermatogonia. / von Kopylow, Kathrein; Staege, Hannah; Spiess, Andrej-Nikolai; Schulze, Wolfgang; Will, Hans; Primig, Michael; Kirchhoff, Christiane.

in: REPRODUCTION, Jahrgang 143, Nr. 1, 1, 2012, S. 45-57.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

von Kopylow, K, Staege, H, Spiess, A-N, Schulze, W, Will, H, Primig, M & Kirchhoff, C 2012, 'Differential marker protein expression specifies rarefaction zone-containing human A (dark) spermatogonia.', REPRODUCTION, Jg. 143, Nr. 1, 1, S. 45-57. <http://www.ncbi.nlm.nih.gov/pubmed/22046055?dopt=Citation>

APA

von Kopylow, K., Staege, H., Spiess, A-N., Schulze, W., Will, H., Primig, M., & Kirchhoff, C. (2012). Differential marker protein expression specifies rarefaction zone-containing human A (dark) spermatogonia. REPRODUCTION, 143(1), 45-57. [1]. http://www.ncbi.nlm.nih.gov/pubmed/22046055?dopt=Citation

Vancouver

von Kopylow K, Staege H, Spiess A-N, Schulze W, Will H, Primig M et al. Differential marker protein expression specifies rarefaction zone-containing human A (dark) spermatogonia. REPRODUCTION. 2012;143(1):45-57. 1.

Bibtex

@article{cd690e8d157244608149a77e19bbdc1d,
title = "Differential marker protein expression specifies rarefaction zone-containing human A (dark) spermatogonia.",
abstract = "It is unclear whether the distinct nuclear morphologies of human A(dark) (Ad) and A(pale) (Ap) spermatogonia are manifestations of different stages of germ cell development or phases of the mitotic cycle, or whether they may reflect still unknown molecular differences. According to the classical description by Clermont, human dark type A spermatogonium (Ad) may contain one, sometimes two or three nuclear 'vacuolar spaces' representing chromatin rarefaction zones. These structures were readily discerned in paraffin sections of human testis tissue during immunohistochemical and immunofluorescence analyses and thus represented robust morphological markers for our study. While a majority of the marker proteins tested did not discriminate between spermatogonia with and without chromatin rarefaction zones, doublesex- and mab-3-related transcription factor (DMRT1), tyrosine kinase receptor c-Kit/CD117 (KIT) and proliferation-associated antigen Ki-67 (KI-67) appeared to be restricted to subtypes which lacked the rarefaction zones. Conversely, exosome component 10 (EXOSC10) was found to accumulate within the rarefaction zones, which points to a possible role of this nuclear domain in RNA processing.",
author = "{von Kopylow}, Kathrein and Hannah Staege and Andrej-Nikolai Spiess and Wolfgang Schulze and Hans Will and Michael Primig and Christiane Kirchhoff",
year = "2012",
language = "English",
volume = "143",
pages = "45--57",
journal = "REPRODUCTION",
issn = "1470-1626",
publisher = "BioScientifica Ltd.",
number = "1",

}

RIS

TY - JOUR

T1 - Differential marker protein expression specifies rarefaction zone-containing human A (dark) spermatogonia.

AU - von Kopylow, Kathrein

AU - Staege, Hannah

AU - Spiess, Andrej-Nikolai

AU - Schulze, Wolfgang

AU - Will, Hans

AU - Primig, Michael

AU - Kirchhoff, Christiane

PY - 2012

Y1 - 2012

N2 - It is unclear whether the distinct nuclear morphologies of human A(dark) (Ad) and A(pale) (Ap) spermatogonia are manifestations of different stages of germ cell development or phases of the mitotic cycle, or whether they may reflect still unknown molecular differences. According to the classical description by Clermont, human dark type A spermatogonium (Ad) may contain one, sometimes two or three nuclear 'vacuolar spaces' representing chromatin rarefaction zones. These structures were readily discerned in paraffin sections of human testis tissue during immunohistochemical and immunofluorescence analyses and thus represented robust morphological markers for our study. While a majority of the marker proteins tested did not discriminate between spermatogonia with and without chromatin rarefaction zones, doublesex- and mab-3-related transcription factor (DMRT1), tyrosine kinase receptor c-Kit/CD117 (KIT) and proliferation-associated antigen Ki-67 (KI-67) appeared to be restricted to subtypes which lacked the rarefaction zones. Conversely, exosome component 10 (EXOSC10) was found to accumulate within the rarefaction zones, which points to a possible role of this nuclear domain in RNA processing.

AB - It is unclear whether the distinct nuclear morphologies of human A(dark) (Ad) and A(pale) (Ap) spermatogonia are manifestations of different stages of germ cell development or phases of the mitotic cycle, or whether they may reflect still unknown molecular differences. According to the classical description by Clermont, human dark type A spermatogonium (Ad) may contain one, sometimes two or three nuclear 'vacuolar spaces' representing chromatin rarefaction zones. These structures were readily discerned in paraffin sections of human testis tissue during immunohistochemical and immunofluorescence analyses and thus represented robust morphological markers for our study. While a majority of the marker proteins tested did not discriminate between spermatogonia with and without chromatin rarefaction zones, doublesex- and mab-3-related transcription factor (DMRT1), tyrosine kinase receptor c-Kit/CD117 (KIT) and proliferation-associated antigen Ki-67 (KI-67) appeared to be restricted to subtypes which lacked the rarefaction zones. Conversely, exosome component 10 (EXOSC10) was found to accumulate within the rarefaction zones, which points to a possible role of this nuclear domain in RNA processing.

M3 - SCORING: Journal article

VL - 143

SP - 45

EP - 57

JO - REPRODUCTION

JF - REPRODUCTION

SN - 1470-1626

IS - 1

M1 - 1

ER -