Differential gene expression profiling of vocal fold polyps and Reinke's edema by complementary DNA microarray.
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Differential gene expression profiling of vocal fold polyps and Reinke's edema by complementary DNA microarray. / Duflo, Suzy M; Thibeault, Susan L; Li, Wenhua; Smith, Marshall E; Goetz, Schade; Hess, Markus.
in: ANN OTO RHINOL LARYN, Jahrgang 115, Nr. 9, 9, 2006, S. 703-714.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Differential gene expression profiling of vocal fold polyps and Reinke's edema by complementary DNA microarray.
AU - Duflo, Suzy M
AU - Thibeault, Susan L
AU - Li, Wenhua
AU - Smith, Marshall E
AU - Goetz, Schade
AU - Hess, Markus
PY - 2006
Y1 - 2006
N2 - OBJECTIVES: Our purpose was to determine whether complementary DNA (cDNA) microarray analysis (MA) can establish distinct gene expression profiles for 2 phenotypically similar vocal fold lesions: Reinke's edema (RE) and polyps. Established transcript profiles can provide insight into the molecular and cellular processes involved in these diseases. METHODS: Eleven RE specimens and 17 polyps were analyzed with MA for 8,745 genes. Further MA profiling was attempted within each lesion group to identify molecular markers for reflux exposure and smoking. Prediction analysis was used to predict lesion classification for 2 unclassified samples. A real-time polymerase chain reaction was performed to corroborate MA transcript levels for selected significant genes. RESULTS: Sixty-five genes were found to differentiate RE and polyps (p = .0088). For RE, 19 genes were differentiated for reflux exposure (p = .016). No genes were found to differentiate smokers from nonsmokers. For polyps, no genes were found to differentiate for reflux (p = .16) and smoking (p = .565). Categorization of unclassified lesions was possible with a minimum of 13 genes. CONCLUSIONS: We demonstrate the feasibility of benign lesion classification based on MA. Microarray analysis is useful not only for improving diagnosis and classification of such lesions, but also for potentially generating prognostic indicators and targets for therapy.
AB - OBJECTIVES: Our purpose was to determine whether complementary DNA (cDNA) microarray analysis (MA) can establish distinct gene expression profiles for 2 phenotypically similar vocal fold lesions: Reinke's edema (RE) and polyps. Established transcript profiles can provide insight into the molecular and cellular processes involved in these diseases. METHODS: Eleven RE specimens and 17 polyps were analyzed with MA for 8,745 genes. Further MA profiling was attempted within each lesion group to identify molecular markers for reflux exposure and smoking. Prediction analysis was used to predict lesion classification for 2 unclassified samples. A real-time polymerase chain reaction was performed to corroborate MA transcript levels for selected significant genes. RESULTS: Sixty-five genes were found to differentiate RE and polyps (p = .0088). For RE, 19 genes were differentiated for reflux exposure (p = .016). No genes were found to differentiate smokers from nonsmokers. For polyps, no genes were found to differentiate for reflux (p = .16) and smoking (p = .565). Categorization of unclassified lesions was possible with a minimum of 13 genes. CONCLUSIONS: We demonstrate the feasibility of benign lesion classification based on MA. Microarray analysis is useful not only for improving diagnosis and classification of such lesions, but also for potentially generating prognostic indicators and targets for therapy.
M3 - SCORING: Zeitschriftenaufsatz
VL - 115
SP - 703
EP - 714
JO - ANN OTO RHINOL LARYN
JF - ANN OTO RHINOL LARYN
SN - 0003-4894
IS - 9
M1 - 9
ER -
