Differential expression of microRNAs following cardiopulmonary bypass in children with congenital heart diseases

TY - JOUR

T1 - Differential expression of microRNAs following cardiopulmonary bypass in children with congenital heart diseases

AU - Abu-Halima, Masood

AU - Poryo, Martin

AU - Ludwig, Nicole

AU - Mark, Janine

AU - Marsollek, Ina

AU - Giebels, Christian

AU - Petersen, Johannes

AU - Schäfers, Hans-Joachim

AU - Grundmann, Ulrich

AU - Pickardt, Thomas

AU - Keller, Andreas

AU - Meese, Eckart

AU - Abdul-Khaliq, Hashim

PY - 2017/5/30

Y1 - 2017/5/30

N2 - BACKGROUND: Children with congenital heart defects (CHDs) are at high risk for myocardial failure after operative procedures with cardiopulmonary bypass (CPB). Recent studies suggest that microRNAs (miRNA) are involved in the development of CHDs and myocardial failure. Therefore, the aim of this study was to determine alterations in the miRNA profile in heart tissue after cardiac surgery using CPB.METHODS: In total, 14 tissue samples from right atrium were collected from patients before and after connection of the CPB. SurePrint™ 8 × 60K Human v21 miRNA array and quantitative reverse transcription-polymerase chain reaction (RT-qPCR) were employed to determine the miRNA expression profile from three patients before and after connection of the CPB. Enrichment analyses of altered miRNA expression were predicted using bioinformatic tools.RESULTS: According to miRNA array, a total of 90 miRNAs were significantly altered including 29 miRNAs with increased and 61 miRNAs with decreased expression after de-connection of CPB (n = 3) compared to before CPB (n = 3). Seven miRNAs had been validated using RT-qPCR in an independent cohort of 11 patients. Enrichment analyses applying the KEGG database displayed the highest correlation for signaling pathways, cellular community, cardiovascular disease and circulatory system.CONCLUSION: Our result identified the overall changes of the miRNome in right atrium tissue of patients with CHDs after CPB. The differentially altered miRNAs lay a good foundation for further understanding of the molecular function of changed miRNAs in regulating CHDs and after CPB in particular.

AB - BACKGROUND: Children with congenital heart defects (CHDs) are at high risk for myocardial failure after operative procedures with cardiopulmonary bypass (CPB). Recent studies suggest that microRNAs (miRNA) are involved in the development of CHDs and myocardial failure. Therefore, the aim of this study was to determine alterations in the miRNA profile in heart tissue after cardiac surgery using CPB.METHODS: In total, 14 tissue samples from right atrium were collected from patients before and after connection of the CPB. SurePrint™ 8 × 60K Human v21 miRNA array and quantitative reverse transcription-polymerase chain reaction (RT-qPCR) were employed to determine the miRNA expression profile from three patients before and after connection of the CPB. Enrichment analyses of altered miRNA expression were predicted using bioinformatic tools.RESULTS: According to miRNA array, a total of 90 miRNAs were significantly altered including 29 miRNAs with increased and 61 miRNAs with decreased expression after de-connection of CPB (n = 3) compared to before CPB (n = 3). Seven miRNAs had been validated using RT-qPCR in an independent cohort of 11 patients. Enrichment analyses applying the KEGG database displayed the highest correlation for signaling pathways, cellular community, cardiovascular disease and circulatory system.CONCLUSION: Our result identified the overall changes of the miRNome in right atrium tissue of patients with CHDs after CPB. The differentially altered miRNAs lay a good foundation for further understanding of the molecular function of changed miRNAs in regulating CHDs and after CPB in particular.

KW - Cardiopulmonary Bypass

KW - Child

KW - Child, Preschool

KW - Cluster Analysis

KW - Cohort Studies

KW - Computational Biology

KW - Female

KW - Gene Expression Profiling

KW - Gene Expression Regulation

KW - Heart Atria/metabolism

KW - Heart Defects, Congenital/metabolism

KW - Humans

KW - Infant

KW - Male

KW - MicroRNAs/metabolism

KW - Myocardium/pathology

KW - Oligonucleotide Array Sequence Analysis

KW - Time Factors

KW - Tissue Distribution

U2 - 10.1186/s12967-017-1213-9

DO - 10.1186/s12967-017-1213-9

M3 - SCORING: Journal article

C2 - 28558735

VL - 15

SP - 117

JO - J TRANSL MED

JF - J TRANSL MED

SN - 1479-5876

IS - 1

ER -