Different signatures of miR-16, miR-30b and miR-93 in exosomes from breast cancer and DCIS patients
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Different signatures of miR-16, miR-30b and miR-93 in exosomes from breast cancer and DCIS patients. / Ni, Qingtao; Stevic, Ines; Pan, Chi; Müller, Volkmar; Oliviera-Ferrer, Leticia; Pantel, Klaus; Schwarzenbach, Heidi.
in: SCI REP-UK, Jahrgang 8, 28.08.2018, S. 12974.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Different signatures of miR-16, miR-30b and miR-93 in exosomes from breast cancer and DCIS patients
AU - Ni, Qingtao
AU - Stevic, Ines
AU - Pan, Chi
AU - Müller, Volkmar
AU - Oliviera-Ferrer, Leticia
AU - Pantel, Klaus
AU - Schwarzenbach, Heidi
PY - 2018/8/28
Y1 - 2018/8/28
N2 - Loading of microRNAs (miRNAs) into exosomes that are involved in cellular communication is a selective process. The current study investigates whether the enrichment of miRNAs in exosomes reflects the pathogenesis of breast cancer (BC) and ductal carcinoma in situ (DCIS). The levels of miRNAs were quantified in exosomes from plasma of 32 BC patients, 8 DCIS patients and 8 healthy women by TaqMan real-time PCR-based miRNA array cards containing 47 different miRNAs. Then, exosomal miR-16, miR-30b and miR-93 that displayed deregulation in the arrays were selected and analyzed in 111 BC patients, 42 DCIS patients and 39 healthy women by TaqMan real-time PCR. Identification of exosomes was performed by Western blot. The levels of exosomal miR-16 were higher in plasma of BC (p = 0.034) and DCIS (p = 0.047) patients than healthy women, and were associated with estrogen (p = 0.004) and progesterone (p = 0.008) receptor status. Particularly, in estrogen-positive patients miR-16 was significantly enriched in exosomes (p = 0.0001). Lower levels of exosomal miR-30b were associated with recurrence (p = 0.034). Exosomal miR-93 was upregulated in DCIS patients (p = 0.001). Our findings suggest that different signatures of miR-16, miR-30b and miR-93 in exosomes from BC and DCIS patients are associated with a particular biology of breast tumors.
AB - Loading of microRNAs (miRNAs) into exosomes that are involved in cellular communication is a selective process. The current study investigates whether the enrichment of miRNAs in exosomes reflects the pathogenesis of breast cancer (BC) and ductal carcinoma in situ (DCIS). The levels of miRNAs were quantified in exosomes from plasma of 32 BC patients, 8 DCIS patients and 8 healthy women by TaqMan real-time PCR-based miRNA array cards containing 47 different miRNAs. Then, exosomal miR-16, miR-30b and miR-93 that displayed deregulation in the arrays were selected and analyzed in 111 BC patients, 42 DCIS patients and 39 healthy women by TaqMan real-time PCR. Identification of exosomes was performed by Western blot. The levels of exosomal miR-16 were higher in plasma of BC (p = 0.034) and DCIS (p = 0.047) patients than healthy women, and were associated with estrogen (p = 0.004) and progesterone (p = 0.008) receptor status. Particularly, in estrogen-positive patients miR-16 was significantly enriched in exosomes (p = 0.0001). Lower levels of exosomal miR-30b were associated with recurrence (p = 0.034). Exosomal miR-93 was upregulated in DCIS patients (p = 0.001). Our findings suggest that different signatures of miR-16, miR-30b and miR-93 in exosomes from BC and DCIS patients are associated with a particular biology of breast tumors.
KW - Journal Article
U2 - 10.1038/s41598-018-31108-y
DO - 10.1038/s41598-018-31108-y
M3 - SCORING: Journal article
C2 - 30154547
VL - 8
SP - 12974
JO - SCI REP-UK
JF - SCI REP-UK
SN - 2045-2322
ER -
