PortalPublikationenDifferent patterns of nicotinic acetylcholine receptor subun...

Different patterns of nicotinic acetylcholine receptor subunit transcription in human thymus

Standard

Different patterns of nicotinic acetylcholine receptor subunit transcription in human thymus. / Bruno, Roxana; Sabater, Lidia; Tolosa, Eva; Sospedra, Mireia; Ferrer-Francesch, Xavier; Coll, Jaume; Foz, Marius; Melms, Arthur; Pujol-Borrell, Ricardo.

in: J NEUROIMMUNOL, Jahrgang 149, Nr. 1-2, 01.04.2004, S. 147-59.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Bruno, R, Sabater, L, Tolosa, E, Sospedra, M, Ferrer-Francesch, X, Coll, J, Foz, M, Melms, A & Pujol-Borrell, R 2004, 'Different patterns of nicotinic acetylcholine receptor subunit transcription in human thymus', J NEUROIMMUNOL, Jg. 149, Nr. 1-2, S. 147-59. https://doi.org/10.1016/j.jneuroim.2003.11.022

APA

Bruno, R., Sabater, L., Tolosa, E., Sospedra, M., Ferrer-Francesch, X., Coll, J., Foz, M., Melms, A., & Pujol-Borrell, R. (2004). Different patterns of nicotinic acetylcholine receptor subunit transcription in human thymus. J NEUROIMMUNOL, 149(1-2), 147-59. https://doi.org/10.1016/j.jneuroim.2003.11.022

Vancouver

Bruno R, Sabater L, Tolosa E, Sospedra M, Ferrer-Francesch X, Coll J et al. Different patterns of nicotinic acetylcholine receptor subunit transcription in human thymus. J NEUROIMMUNOL. 2004 Apr 1;149(1-2):147-59. https://doi.org/10.1016/j.jneuroim.2003.11.022

Bibtex

@article{9961c5e3a5124188bae00a385e6e0768,
title = "Different patterns of nicotinic acetylcholine receptor subunit transcription in human thymus",
abstract = "Clinical observations suggest that the thymus is strongly implicated in the pathogenesis of myasthenia gravis (MG), but questions such as the level and location of nicotinic acetylcholine receptor (AChR) subunit expression that are fundamental to postulate any pathogenic mechanism, remain controversial. We have re-examined this question by combining calibrated RT-PCR and real-time PCR to study nicotinic AChR subunit mRNA expression in a panel of normal and myasthenic thymi. The results suggest that the expression of the different AChR subunits follows three distinct patterns: constitutive for, neonatal for gamma and individually variable for alpha1, beta1 and delta. Experiments using confocal laser microdissection suggest that AChR is mainly expressed in the medullary compartment of the thymus but there is not a clear compartmentalization of subunit expression. The different patterns of subunit expression may influence decisively the level of central tolerance to the subunits and explain the focusing of the T cell response to the alpha and gamma subunits.",
keywords = "Adolescent, Adult, Aged, Blotting, Southern, Desmin, Epithelial Cells, Fluorescent Antibody Technique, Gene Expression, Humans, Microdissection, Middle Aged, Muscles, Myasthenia Gravis, Protein Subunits, RNA, Messenger, Receptors, Nicotinic, Reverse Transcriptase Polymerase Chain Reaction, Thymoma, Thymus Gland, Thymus Neoplasms, Time Factors, Tissue Distribution, Transcription, Genetic",
author = "Roxana Bruno and Lidia Sabater and Eva Tolosa and Mireia Sospedra and Xavier Ferrer-Francesch and Jaume Coll and Marius Foz and Arthur Melms and Ricardo Pujol-Borrell",
year = "2004",
month = apr,
day = "1",
doi = "10.1016/j.jneuroim.2003.11.022",
language = "English",
volume = "149",
pages = "147--59",
journal = "J NEUROIMMUNOL",
issn = "0165-5728",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Different patterns of nicotinic acetylcholine receptor subunit transcription in human thymus

AU - Bruno, Roxana

AU - Sabater, Lidia

AU - Tolosa, Eva

AU - Sospedra, Mireia

AU - Ferrer-Francesch, Xavier

AU - Coll, Jaume

AU - Foz, Marius

AU - Melms, Arthur

AU - Pujol-Borrell, Ricardo

PY - 2004/4/1

Y1 - 2004/4/1

N2 - Clinical observations suggest that the thymus is strongly implicated in the pathogenesis of myasthenia gravis (MG), but questions such as the level and location of nicotinic acetylcholine receptor (AChR) subunit expression that are fundamental to postulate any pathogenic mechanism, remain controversial. We have re-examined this question by combining calibrated RT-PCR and real-time PCR to study nicotinic AChR subunit mRNA expression in a panel of normal and myasthenic thymi. The results suggest that the expression of the different AChR subunits follows three distinct patterns: constitutive for, neonatal for gamma and individually variable for alpha1, beta1 and delta. Experiments using confocal laser microdissection suggest that AChR is mainly expressed in the medullary compartment of the thymus but there is not a clear compartmentalization of subunit expression. The different patterns of subunit expression may influence decisively the level of central tolerance to the subunits and explain the focusing of the T cell response to the alpha and gamma subunits.

AB - Clinical observations suggest that the thymus is strongly implicated in the pathogenesis of myasthenia gravis (MG), but questions such as the level and location of nicotinic acetylcholine receptor (AChR) subunit expression that are fundamental to postulate any pathogenic mechanism, remain controversial. We have re-examined this question by combining calibrated RT-PCR and real-time PCR to study nicotinic AChR subunit mRNA expression in a panel of normal and myasthenic thymi. The results suggest that the expression of the different AChR subunits follows three distinct patterns: constitutive for, neonatal for gamma and individually variable for alpha1, beta1 and delta. Experiments using confocal laser microdissection suggest that AChR is mainly expressed in the medullary compartment of the thymus but there is not a clear compartmentalization of subunit expression. The different patterns of subunit expression may influence decisively the level of central tolerance to the subunits and explain the focusing of the T cell response to the alpha and gamma subunits.

KW - Adolescent

KW - Adult

KW - Aged

KW - Blotting, Southern

KW - Desmin

KW - Epithelial Cells

KW - Fluorescent Antibody Technique

KW - Gene Expression

KW - Humans

KW - Microdissection

KW - Middle Aged

KW - Muscles

KW - Myasthenia Gravis

KW - Protein Subunits

KW - RNA, Messenger

KW - Receptors, Nicotinic

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Thymoma

KW - Thymus Gland

KW - Thymus Neoplasms

KW - Time Factors

KW - Tissue Distribution

KW - Transcription, Genetic

U2 - 10.1016/j.jneuroim.2003.11.022

DO - 10.1016/j.jneuroim.2003.11.022

M3 - SCORING: Journal article

C2 - 15020075

VL - 149

SP - 147

EP - 159

JO - J NEUROIMMUNOL

JF - J NEUROIMMUNOL

SN - 0165-5728

IS - 1-2

ER -