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Different Forms of TFF3 in the Human Endocervix, including a Complex with IgG Fc Binding Protein (FCGBP), and Further Aspects of the Cervico-Vaginal Innate Immune Barrier

Standard

Different Forms of TFF3 in the Human Endocervix, including a Complex with IgG Fc Binding Protein (FCGBP), and Further Aspects of the Cervico-Vaginal Innate Immune Barrier. / Laskou, Aikaterini; Znalesniak, Eva B; Harder, Sönke; Schlüter, Hartmut; Jechorek, Dörthe; Langer, Kathrin; Strecker, Carina; Matthes, Claudia; Tchaikovski, Svetlana N; Hoffmann, Werner.

in: INT J MOL SCI, Jahrgang 25, Nr. 4, 14.02.2024.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Laskou, A, Znalesniak, EB, Harder, S, Schlüter, H, Jechorek, D, Langer, K, Strecker, C, Matthes, C, Tchaikovski, SN & Hoffmann, W 2024, 'Different Forms of TFF3 in the Human Endocervix, including a Complex with IgG Fc Binding Protein (FCGBP), and Further Aspects of the Cervico-Vaginal Innate Immune Barrier', INT J MOL SCI, Jg. 25, Nr. 4. https://doi.org/10.3390/ijms25042287

APA

Laskou, A., Znalesniak, E. B., Harder, S., Schlüter, H., Jechorek, D., Langer, K., Strecker, C., Matthes, C., Tchaikovski, S. N., & Hoffmann, W. (2024). Different Forms of TFF3 in the Human Endocervix, including a Complex with IgG Fc Binding Protein (FCGBP), and Further Aspects of the Cervico-Vaginal Innate Immune Barrier. INT J MOL SCI, 25(4). https://doi.org/10.3390/ijms25042287

Vancouver

Laskou A, Znalesniak EB, Harder S, Schlüter H, Jechorek D, Langer K et al. Different Forms of TFF3 in the Human Endocervix, including a Complex with IgG Fc Binding Protein (FCGBP), and Further Aspects of the Cervico-Vaginal Innate Immune Barrier. INT J MOL SCI. 2024 Feb 14;25(4). https://doi.org/10.3390/ijms25042287

Bibtex

@article{e378863f6c624da2b8531ecb9dc32081,
title = "Different Forms of TFF3 in the Human Endocervix, including a Complex with IgG Fc Binding Protein (FCGBP), and Further Aspects of the Cervico-Vaginal Innate Immune Barrier",
abstract = "TFF3 is a typical secretory poplypeptide of mucous epithelia belonging to the trefoil factor family (TFF) of lectins. In the intestine, respiratory tract, and saliva, TFF3 mainly exists as a high-molecular-mass complex with IgG Fc binding protein (FCGBP), which is indicative of a role in mucosal innate immunity. For the first time, we identified different forms of TFF3 in the endocervix, i.e., monomeric and homodimeric TFF3, as well as a high-molecular-mass TFF3-FCGBP complex; the latter also exists in a hardly soluble form. Immunohistochemistry co-localized TFF3 and FCGBP. Expression analyses of endocervical and post-menopausal vaginal specimens revealed a lack of mucin and TFF3 transcripts in the vaginal specimens. In contrast, genes encoding other typical components of the innate immune defense were expressed in both the endocervix and vagina. Of note, FCGBP is possibly fucosylated. Endocervical specimens from transgender individuals after hormonal therapy showed diminished expression, particularly of FCGBP. Furthermore, mucus swabs from the endocervix and vagina were analyzed concerning TFF3, FCGBP, and lysozyme. It was the aim of this study to illuminate several aspects of the cervico-vaginal innate immune barrier, which is clinically relevant as bacterial and viral infections are also linked to infertility, pre-term birth and cervical cancer.",
keywords = "Female, Humans, Carrier Proteins, Cell Adhesion Molecules/metabolism, Cervix Uteri/immunology, Immunity, Innate, Immunoglobulin G/metabolism, Mucins/metabolism, Trefoil Factor-2/metabolism, Trefoil Factor-3/genetics, Vagina/immunology",
author = "Aikaterini Laskou and Znalesniak, {Eva B} and S{\"o}nke Harder and Hartmut Schl{\"u}ter and D{\"o}rthe Jechorek and Kathrin Langer and Carina Strecker and Claudia Matthes and Tchaikovski, {Svetlana N} and Werner Hoffmann",
year = "2024",
month = feb,
day = "14",
doi = "10.3390/ijms25042287",
language = "English",
volume = "25",
journal = "INT J MOL SCI",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "4",

}

RIS

TY - JOUR

T1 - Different Forms of TFF3 in the Human Endocervix, including a Complex with IgG Fc Binding Protein (FCGBP), and Further Aspects of the Cervico-Vaginal Innate Immune Barrier

AU - Laskou, Aikaterini

AU - Znalesniak, Eva B

AU - Harder, Sönke

AU - Schlüter, Hartmut

AU - Jechorek, Dörthe

AU - Langer, Kathrin

AU - Strecker, Carina

AU - Matthes, Claudia

AU - Tchaikovski, Svetlana N

AU - Hoffmann, Werner

PY - 2024/2/14

Y1 - 2024/2/14

N2 - TFF3 is a typical secretory poplypeptide of mucous epithelia belonging to the trefoil factor family (TFF) of lectins. In the intestine, respiratory tract, and saliva, TFF3 mainly exists as a high-molecular-mass complex with IgG Fc binding protein (FCGBP), which is indicative of a role in mucosal innate immunity. For the first time, we identified different forms of TFF3 in the endocervix, i.e., monomeric and homodimeric TFF3, as well as a high-molecular-mass TFF3-FCGBP complex; the latter also exists in a hardly soluble form. Immunohistochemistry co-localized TFF3 and FCGBP. Expression analyses of endocervical and post-menopausal vaginal specimens revealed a lack of mucin and TFF3 transcripts in the vaginal specimens. In contrast, genes encoding other typical components of the innate immune defense were expressed in both the endocervix and vagina. Of note, FCGBP is possibly fucosylated. Endocervical specimens from transgender individuals after hormonal therapy showed diminished expression, particularly of FCGBP. Furthermore, mucus swabs from the endocervix and vagina were analyzed concerning TFF3, FCGBP, and lysozyme. It was the aim of this study to illuminate several aspects of the cervico-vaginal innate immune barrier, which is clinically relevant as bacterial and viral infections are also linked to infertility, pre-term birth and cervical cancer.

AB - TFF3 is a typical secretory poplypeptide of mucous epithelia belonging to the trefoil factor family (TFF) of lectins. In the intestine, respiratory tract, and saliva, TFF3 mainly exists as a high-molecular-mass complex with IgG Fc binding protein (FCGBP), which is indicative of a role in mucosal innate immunity. For the first time, we identified different forms of TFF3 in the endocervix, i.e., monomeric and homodimeric TFF3, as well as a high-molecular-mass TFF3-FCGBP complex; the latter also exists in a hardly soluble form. Immunohistochemistry co-localized TFF3 and FCGBP. Expression analyses of endocervical and post-menopausal vaginal specimens revealed a lack of mucin and TFF3 transcripts in the vaginal specimens. In contrast, genes encoding other typical components of the innate immune defense were expressed in both the endocervix and vagina. Of note, FCGBP is possibly fucosylated. Endocervical specimens from transgender individuals after hormonal therapy showed diminished expression, particularly of FCGBP. Furthermore, mucus swabs from the endocervix and vagina were analyzed concerning TFF3, FCGBP, and lysozyme. It was the aim of this study to illuminate several aspects of the cervico-vaginal innate immune barrier, which is clinically relevant as bacterial and viral infections are also linked to infertility, pre-term birth and cervical cancer.

KW - Female

KW - Humans

KW - Carrier Proteins

KW - Cell Adhesion Molecules/metabolism

KW - Cervix Uteri/immunology

KW - Immunity, Innate

KW - Immunoglobulin G/metabolism

KW - Mucins/metabolism

KW - Trefoil Factor-2/metabolism

KW - Trefoil Factor-3/genetics

KW - Vagina/immunology

U2 - 10.3390/ijms25042287

DO - 10.3390/ijms25042287

M3 - SCORING: Journal article

C2 - 38396964

VL - 25

JO - INT J MOL SCI

JF - INT J MOL SCI

SN - 1661-6596

IS - 4

ER -