Different Forms of TFF3 in the Human Endocervix, including a Complex with IgG Fc Binding Protein (FCGBP), and Further Aspects of the Cervico-Vaginal Innate Immune Barrier
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Different Forms of TFF3 in the Human Endocervix, including a Complex with IgG Fc Binding Protein (FCGBP), and Further Aspects of the Cervico-Vaginal Innate Immune Barrier. / Laskou, Aikaterini; Znalesniak, Eva B; Harder, Sönke; Schlüter, Hartmut; Jechorek, Dörthe; Langer, Kathrin; Strecker, Carina; Matthes, Claudia; Tchaikovski, Svetlana N; Hoffmann, Werner.
in: INT J MOL SCI, Jahrgang 25, Nr. 4, 14.02.2024.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Different Forms of TFF3 in the Human Endocervix, including a Complex with IgG Fc Binding Protein (FCGBP), and Further Aspects of the Cervico-Vaginal Innate Immune Barrier
AU - Laskou, Aikaterini
AU - Znalesniak, Eva B
AU - Harder, Sönke
AU - Schlüter, Hartmut
AU - Jechorek, Dörthe
AU - Langer, Kathrin
AU - Strecker, Carina
AU - Matthes, Claudia
AU - Tchaikovski, Svetlana N
AU - Hoffmann, Werner
PY - 2024/2/14
Y1 - 2024/2/14
N2 - TFF3 is a typical secretory poplypeptide of mucous epithelia belonging to the trefoil factor family (TFF) of lectins. In the intestine, respiratory tract, and saliva, TFF3 mainly exists as a high-molecular-mass complex with IgG Fc binding protein (FCGBP), which is indicative of a role in mucosal innate immunity. For the first time, we identified different forms of TFF3 in the endocervix, i.e., monomeric and homodimeric TFF3, as well as a high-molecular-mass TFF3-FCGBP complex; the latter also exists in a hardly soluble form. Immunohistochemistry co-localized TFF3 and FCGBP. Expression analyses of endocervical and post-menopausal vaginal specimens revealed a lack of mucin and TFF3 transcripts in the vaginal specimens. In contrast, genes encoding other typical components of the innate immune defense were expressed in both the endocervix and vagina. Of note, FCGBP is possibly fucosylated. Endocervical specimens from transgender individuals after hormonal therapy showed diminished expression, particularly of FCGBP. Furthermore, mucus swabs from the endocervix and vagina were analyzed concerning TFF3, FCGBP, and lysozyme. It was the aim of this study to illuminate several aspects of the cervico-vaginal innate immune barrier, which is clinically relevant as bacterial and viral infections are also linked to infertility, pre-term birth and cervical cancer.
AB - TFF3 is a typical secretory poplypeptide of mucous epithelia belonging to the trefoil factor family (TFF) of lectins. In the intestine, respiratory tract, and saliva, TFF3 mainly exists as a high-molecular-mass complex with IgG Fc binding protein (FCGBP), which is indicative of a role in mucosal innate immunity. For the first time, we identified different forms of TFF3 in the endocervix, i.e., monomeric and homodimeric TFF3, as well as a high-molecular-mass TFF3-FCGBP complex; the latter also exists in a hardly soluble form. Immunohistochemistry co-localized TFF3 and FCGBP. Expression analyses of endocervical and post-menopausal vaginal specimens revealed a lack of mucin and TFF3 transcripts in the vaginal specimens. In contrast, genes encoding other typical components of the innate immune defense were expressed in both the endocervix and vagina. Of note, FCGBP is possibly fucosylated. Endocervical specimens from transgender individuals after hormonal therapy showed diminished expression, particularly of FCGBP. Furthermore, mucus swabs from the endocervix and vagina were analyzed concerning TFF3, FCGBP, and lysozyme. It was the aim of this study to illuminate several aspects of the cervico-vaginal innate immune barrier, which is clinically relevant as bacterial and viral infections are also linked to infertility, pre-term birth and cervical cancer.
KW - Female
KW - Humans
KW - Carrier Proteins
KW - Cell Adhesion Molecules/metabolism
KW - Cervix Uteri/immunology
KW - Immunity, Innate
KW - Immunoglobulin G/metabolism
KW - Mucins/metabolism
KW - Trefoil Factor-2/metabolism
KW - Trefoil Factor-3/genetics
KW - Vagina/immunology
U2 - 10.3390/ijms25042287
DO - 10.3390/ijms25042287
M3 - SCORING: Journal article
C2 - 38396964
VL - 25
JO - INT J MOL SCI
JF - INT J MOL SCI
SN - 1661-6596
IS - 4
ER -